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FlAsH-EDT2

2022-10-31 17:28 作者:BIOFOUNT范德生物  | 我要投稿

FlAsH-EDT2(212118-77-9)是一種膜滲透性雙砷染料,可與靶蛋白中的四半胱氨酸序列 (CCPGCC) 共價(jià)結(jié)合。 FlAsH-EDT2 通常用于研究活細(xì)胞或細(xì)胞裂解物中的蛋白質(zhì)運(yùn)輸、折疊和相互作用。。 熒光 :λex 508 nm, λem 528 nm

溶解度SolubilitySoluble in chloroform.

性狀:White to Pale Orange Solid

儲(chǔ)藏條件:-20攝氏度度 Freezer, Under Inert Atmosphere

案例:


Live cell imaging with FlAsH-EDT2, azide-TRAP (1) and diazirine-TRAP (2). HeLa cells were transfected with an expression vector for DMRT1 containing the optimized biarsenical binding tetracysteine motif on its amino terminus (FLN-DMRT1). Cells were then treated with either FlAsH-EDT2 (A and D), compound 1 (B and E) or compound 2 (C and F). The images A–F were generated from the FLN-DMRT1 transfected cells with the background subtracted. Images are 200× (A–C) or enlargements of the boxed areas (D–F).

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