Covalab針對(duì)斑馬魚的抗體研究及相關(guān)應(yīng)用方案
斑馬魚(zebrafish,Danio rerio),又名藍(lán)條魚,是淡水水族觀賞魚,原產(chǎn)于印度、孟加拉國(guó),因全身布滿多條深藍(lán)色縱紋似斑馬而得名。斑馬魚身體延長(zhǎng)而略呈紡錘形,頭小而稍尖,吻較短。由于斑馬魚養(yǎng)殖方便、繁殖周期短、產(chǎn)卵量大、胚胎體外受精、體外發(fā)育、胚體透明,已成為生命科學(xué)研究的新寵。
?
斑馬魚擁有與人相似的組織器官和系統(tǒng),基因和信號(hào)通路與人類高度保守:>85%;生理、發(fā)育和代謝與哺乳動(dòng)物高度相似。個(gè)體小,成魚體長(zhǎng)3-4cm,幼魚體長(zhǎng)只有1-2mm,適合于高通量藥物篩選。發(fā)育周期短?(24?小時(shí)器官便可形成)、實(shí)驗(yàn)周期短?(篩選結(jié)果在一周內(nèi)即可得出)、費(fèi)用低(約為鼠類的?1/10~1/100)、體外受精、透明(可直接觀察藥物對(duì)內(nèi)部器官的作用)、單次產(chǎn)卵數(shù)較高(150~200?枚)以及實(shí)驗(yàn)用藥量小(為小鼠用藥量的?1/100~1/1000)。??
?

目前,斑馬魚已經(jīng)成為繼小鼠和大鼠之后的第三大脊椎類模式生物,在發(fā)育生物學(xué)、遺傳學(xué)、基礎(chǔ)醫(yī)學(xué)、藥理學(xué)、毒理學(xué)、藥物研發(fā)以及生態(tài)環(huán)境評(píng)價(jià)等諸多領(lǐng)域得到了廣泛應(yīng)用。利用斑馬魚,可以研究生命科學(xué)的基礎(chǔ)問題,揭示胚胎和組織器官發(fā)育的分子機(jī)理;可以構(gòu)建人類的各種疾病和腫瘤模型,建立藥物篩選和治療的研究平臺(tái);可以建立毒理學(xué)和水產(chǎn)育種學(xué)模型,研究和解決環(huán)境科學(xué)和農(nóng)業(yè)科學(xué)的重大問題。??
?
艾美捷Covalab針對(duì)斑馬魚的抗體(部分):??
貨號(hào)?名稱?宿主?應(yīng)用類型
mab20361?Actin antibody (HHF35)?小鼠?ELISA, EM, ICC, IHC - F, IHC - P, WB
pab72198?AMPD1 (aa140-190) antibody?兔?IHC - P, WB
pab72199?AMPD1 (aa500-550) antibody?兔?IHC - P, WB
pab74475?ATG4B antibody?兔?IHC - P, WB
pab50264?ATG5 antibody?兔?ICC, IF, IHC - P, WB
pab50234?CAAX prenyl protease 1 homolog antibody?兔?WB
pab0412?Collagen alpha-3 (IV) chain (COL4A3) antibody?兔?ELISA, ICC, IF, WB
pab70092?CRTC1 / MECT1 (aa19-34) antibody?兔?ELISA, IHC - P, WB
pab0736?Cxcr4B antibody?兔?ELISA, IF, WB
mab60115?DNMT1 (4G11-C7) antibody?小鼠?ELISA, WB
pab70910?EPHB2 (Internal) antibody?兔?IHC - P
pab0999-P?Fibrinogen alpha chain antibody?兔?ELISA, WB
pab0992-P?Fibrinogen antibody?兔?ELISA, WB
pab72593?FOXC1 (C-Terminus) antibody?山羊?ELISA, IHC - P, WB
pab76203?FTHFSDC1 / MTHFD1L (Internal) antibody?山羊?ELISA, IHC - P, WB
pab71237?GPR22 (Cytoplasmic Domain) antibody?兔?IHC - P
pab71375?GPR85 (Extracellular Domain) antibody?兔?IHC - P
pab50112?High affinity copper uptake protein 1 antibody?兔?IHC - P
pab52834?JMJD6 antibody?兔?ICC, WB
pab72287?KDM1A / LSD1 (aa100-150) antibody?兔?IHC - P, WB
pab52451?Kv1 antibody?兔?IP, WB
pab52458?Kv1.2 antibody?兔?ICC, WB
pab50207?LC3A antibody?兔?ICC, IF, IHC - P, IP, WB
pab52327?LC3A antibody?兔?FC, ICC, IF, FC, IHC, WB
pab50206?LC3B antibody?兔?FC, ICC, IF, IHC, IP, WB
pab72188?MARCH4 (aa300-350) antibody?兔?IHC - P, WB
pab71719?MCT1 (aa3-14) antibody?兔?ELISA, IHC - P, WB
pab76186?MICU1 / CBARA1 (Internal) antibody?山羊?ELISA, IHC - P, WB
pab72784?MLL4 (C-Terminus) antibody?山羊?ELISA, IHC - P
mab0102-P?Na(+)/K(+) ATPase alpha-1 subunit antibody (A1F6)?小鼠?IHC, IP, WB
pab73827?NTN1 / Netrin 1 (C-Terminus) antibody?山羊?ELISA, IHC - P, WB
pab76086?ORC3L / ORC3 (C-Terminus) antibody?山羊?ELISA, IHC - P, WB
pab0693-IP?P540 Aromatase (Cyp19b) antibody?兔?ELISA, WB, IHC
pab0831-P?Paired box protein Pax-6 antibody?兔?ELISA, WB
pab72933?PPAR Delta (aa430-441) antibody?山羊?ELISA, IHC - P, WB
pab72181?RAB7 (aa90-140) antibody?兔?IHC - P, WB
pab52551?SNX27 antibody?兔?ICC, IF, IHC - P, WB
pab0016?Sodium- and chloride-dependent GABA transporter 2 (GAT-2) antibody?兔?ELISA, IHC, WB
pab50273?TARDBP antibody?兔?ICC, IF, IHC, WB
pab0809-P?Toll-interacting protein antibody?兔?ELISA, WB, IHC
pab0907-P?Tpcn1 antibody?兔?WB, ELISA
pab0908-P?Two pore calcium channel protein 2 (tpcn2) antibody?兔?ELISA, WB
pab0868-P?Two pore channel 3 (TPC3) antibody?兔?ELISA, WB
pab0928-P?VEGF165 antibody?兔?ELISA, WB, IHC
?
斑馬魚抗體應(yīng)用類型:
ChIP:Chromatin immunoprecipitation?染色質(zhì)免疫沉淀
ELISA:Enzyme-linked immunosorbent assay?酶聯(lián)免疫吸附測(cè)定
EM:Electron microscopy?電子顯微鏡
FC:Flow cytometry?流式細(xì)胞術(shù)
IF: Immunofluorescence?免疫熒光
IHC:Immunohistochemistry?免疫組化(IHC-P:IHC, paraffin section?石蠟切片的免疫組化;
IHC-F: IHC, frozen section冰凍切片的免疫組化)
ICC: ?Immunocytochemistry免疫細(xì)胞化學(xué)
IP:Immunoprecipitation?免疫沉淀
WB:Western blot?蛋白質(zhì)印跡
GS: gel shift?凝膠遷移