1.百螢Cy5疊氮化物作用

Cy5疊氮化物已用于標記生物分子，用于熒光成像和其他基于熒光的生化分析。它們廣泛用于標記肽，蛋白質和寡核苷酸等。Cy5 染料是常見的紅色熒光團中的一種。這些多功能熒光團可以耐受3-10的pH范圍，用于生物相關pH的各種應用。該染料還具有DMSO耐受性和光穩(wěn)定性，能夠在不損失性能的情況下從儲存轉移至分析。水溶性在測定緩沖液中對有機溶劑的需要。我們的Cy5染料經過QC測試，可確保高水平的發(fā)色團和活性染料含量。單活性染料適用于蛋白質和寡核苷酸的靶向，標記，雙活性染料更適合于一般標記。建議使用NHS酯染料標記胺基，建議使用馬來酰亞胺染料標記硫醇基團。該Cy5 疊氮化物選擇性地與炔基反應。

2.百螢Cy5疊氮化物參數

Ex(nm)? ? ? ? ?651

Em(nm) ???????670

分 子 量? ? ? ??838.91

溶? ? ??劑? ? ? ??DMSO

儲存條件? ? ???保存在(＜-15℃),盡量避免光照

保??質 期? ? ???12個月

外? ? ? ?觀? ? ? ?藍色固體

3.?百螢Cy5疊氮化物物理性質

?

校正系數(260nm) ????0.02

校正系數(280nm) ????0.03

校正系數(482nm)? ????0.009

校正系數(565nm)? ? ? 0.09

激 ?發(fā)(nm)? ? ? ? ? ? ? ???651

排放量(nm)? ? ? ? ? ? ?? ?670

4.百螢Cy5疊氮化物參考文獻

Cube-shaped theranostic paclitaxel prodrug nanocrystals with surface functionalization of SPC and MPEG-DSPE for imaging and chemotherapy
Authors:?Fuqiang Guo, Jiajia Shang, Hai Zhao, Kangrong Lai, Yang Li, Zhongxiong Fan, Zhenqing Hou, Guanghao Su
Journal:?Colloids and Surfaces B: Biointerfaces (2017)

Light/magnetic hyperthermia triggered drug released from multi-functional thermo-sensitive magnetoliposomes for precise cancer synergetic theranostics
Authors:?Yuxin Guo, Yang Zhang, Jinyuan Ma, Qi Li, Yang Li, Xinyi Zhou, Dan Zhao, Hua Song, Qing Chen, Xuan Zhu
Journal:?Journal of Controlled Release (2017)

Thermo-sensitive hydrogel PLGA-PEG-PLGA as a vaccine delivery system for intramuscular immunization
Authors:?Xiaoyan Wang, Yu Zhang, Wei Xue, Hong Wang, Xiaozhong Qiu, Zonghua Liu
Journal:?Journal of Biomaterials Applications (2017): 923--932

Affinity-Controlled Protein Encapsulation into Sub-30 nm Telodendrimer Nanocarriers by Multivalent and Synergistic Interactions
Authors:?Xu Wang, Changying Shi, Li Zhang, Alexa Bodman, Dandan Guo, Lili Wang, Walter A Hall, Stephan Wilkens, Juntao Luo
Journal:?Biomaterials (2016)

Carboxymethyl Dextran-Stabilized Polyethylenimine-Poly (epsilon-caprolactone) Nanoparticles-Mediated Modulation of MicroRNA-34a Expression via Small-Molecule Modulator for Hepatocellular Carcinoma Therapy
Authors:?Xiongwei Deng, Zhaoxia Yin, Zhixiang Zhou, Yihui Wang, Fang Zhang, Qin Hu, Yishu Yang, Jianqing Lu, Yan Wu, Wang Sheng
Journal:?ACS applied materials & interfaces (2016): 17068--17079

Click-electron microscopy for imaging metabolically tagged nonprotein biomolecules
Authors:?John T Ngo, Stephen R Adams, Thomas J Deerinck, Daniela Boassa, Frances Rodriguez-Rivera, Sakina F Palida, Carolyn R Bertozzi, Mark H Ellisman, Roger Y Tsien
Journal:?Nat Chem Biol (2016): 459--465

Design, synthesis and evaluation of VEGF-siRNA/CRS as a novel vector for gene delivery
Authors:?Wen Zhao, Yifan Zhang, Xueyun Jiang, Chunying Cui
Journal:?Drug Design, Development and Therapy (2016): 3851

Molecular Basis and Consequences of the Cytochrome c-tRNA Interaction
Authors:?Cuiping Liu, Aaron J Stonestrom, Thomas Christian, Jeongsik Yong, Ryuichi Takase, Ya-Ming Hou, Xiaolu Yang
Journal:?Journal of Biological Chemistry (2016): 10426--10436

Determination of the active transport of fucoidan derived from okinawa mozuku across the human intestinal caco-2 cells as assessed by size-exclusion chromatography
Authors:?Takeaki Nagamine, Kou Hayakawa, Kyoumi Nakazato, Masahiko Iha
Journal:?Journal of Chromatography B (2015): 187--193

Multiplexed single-cell in situ RNA analysis by reiterative hybridization
Authors:?Lu Xiao, Jia Guo
Journal:?Analytical Methods (2015): 7290--7295