BioXcell熱銷產(chǎn)品：BE0273 InVivoMab anti-mouse PD-1 (CD279)

產(chǎn)品描述：

BioXcell InVivoMab anti-mouse PD-1 29F.1A12單克隆抗體與小鼠PD-1（也稱為CD279）反應(yīng)。PD-1是一種50-55kDa的細(xì)胞表面受體，由Pdcd1基因編碼，屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和髓細(xì)胞上瞬時(shí)表達(dá)，在成功消除抗原后PD-1的表達(dá)下降。此外，在B細(xì)胞前階段，Pdcd1 mRNA在發(fā)育中的B淋巴細(xì)胞中表達(dá)。PD-1的結(jié)構(gòu)包括ITIM（基于免疫受體酪氨酸的抑制基序），這表明PD-1負(fù)調(diào)控TCR信號(hào)。PD-1通過結(jié)合B7家族的成員PD-L1和PD-L2發(fā)出信號(hào)。在配體結(jié)合后，PD-1信號(hào)傳導(dǎo)抑制T細(xì)胞活化，導(dǎo)致增殖減少，細(xì)胞因子產(chǎn)生和T細(xì)胞死亡。此外，PD-1敲除動(dòng)物表現(xiàn)出擴(kuò)張型心肌病、脾腫大和外周耐受喪失，在小鼠的外周耐受性和預(yù)防自身免疫性疾病中發(fā)揮關(guān)鍵作用。誘導(dǎo)的PD-L1表達(dá)常見于許多腫瘤，包括鱗狀細(xì)胞癌、結(jié)腸癌和乳腺癌。PD-L1過度表達(dá)會(huì)導(dǎo)致腫瘤細(xì)胞對(duì)CD8T細(xì)胞介導(dǎo)的裂解的抗性增加。在黑素瘤的小鼠模型中，可以通過用阻斷PD-L1與其受體PD-1之間相互作用的抗體治療來暫時(shí)抑制腫瘤生長(zhǎng)。由于這些原因，目前正在探索抗PD-1介導(dǎo)的免疫療法作為癌癥治療。與RMP1-14和J43抗體一樣，29F.1A12抗體在體內(nèi)阻斷PD-1與其配體的結(jié)合。

欣博盛生物是BioXcell 國(guó)內(nèi)授權(quán)代理，中國(guó)唯一現(xiàn)貨庫(kù)存中心，如需購(gòu)買BE0273 InVivoMab anti-mouse PD-1 (CD279)或Bioxcell其它產(chǎn)品，請(qǐng)聯(lián)系。

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產(chǎn)品詳情：

產(chǎn)品名稱????InVivoMAb anti-mouse PD-1 (CD279)

產(chǎn)品貨號(hào)????BE0273

產(chǎn)品規(guī)格????1/5/25/50/100mg

反應(yīng)種屬????Mouse

克隆號(hào)????29F.1A12

同種型????Rat IgG2a

免疫原????Recombinant PD-1-Ig fusion protein

實(shí)驗(yàn)應(yīng)用

in vivo blocking of PD-1/PD-L signaling

in vitro PD-1 neutralization

Immunohistochemistry (frozen)

Immunofluorescence

Western blot

Flow cytometry

產(chǎn)品形式????PBS, pH 7.0,Contains no stabilizers or preservatives

純度????>95%, Determined by SDS-PAGE

無菌處理????0.2 μm filtration

純化方式????Protein G

RRID????AB_2687796

分子量????150 kDa

保存條件????抗體原液保存在4°C，不能冷凍保存。

推薦同型對(duì)照????InVivoMAb rat IgG2a isotype control, anti-trinitrophenol（貨號(hào)BE0089）

推薦抗體稀釋液????InVivoPure pH 7.0 Dilution Buffer（貨號(hào)IP0070）

該產(chǎn)品自上市已被多篇SCI文獻(xiàn)引用，品質(zhì)有保證，以下是部分已發(fā)表的文獻(xiàn)引用：

應(yīng)用 ????????????????????文章

體內(nèi)PD-1/PD-L信號(hào)阻斷

(in vivo blocking of PD-1/PD-L signaling)

1.?Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757.

2.?Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499. ?

3.?Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment' Cancer Res 76(5): 999-1008.

體內(nèi)PD-1/PD-L信號(hào)阻斷，流式細(xì)胞術(shù)

（in vivo blocking of PD-1/PD-L signaling, Flow Cytometry）

1.Koyama, S., et al. (2016). 'Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints' Nat Commun 7: 10501.

體外PD-1中和（in vitro PD-1 neutralization）

1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated dendritic cells' J Leukoc Biol 95(4): 621-629.

體內(nèi)PD-1/PD-L信號(hào)阻斷，體外PD-1中和

（in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization）

1.Duraiswamy, J., et al. (2013). 'Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors' Cancer Res 73(12): 3591-3603.

流式細(xì)胞術(shù)（Flow Cytometry）

1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation' J Immunol 188(9): 4217-4225.

免疫熒光，免疫印跡（Immunofluorescence, Western Blot）

1.Chen, L., et al. (2009). 'Role of the immune modulator programmed cell death-1 during development and apoptosis of mouse retinal ganglion cells' Invest Ophthalmol Vis Sci 50(10): 4941-4948.

免疫組織化學(xué)（冷凍）（Immunohistochemistry (frozen)）

1.?Menke, J., et al. (2007). 'Programmed death 1 ligand (PD-L) 1 and PD-L2 limit autoimmune kidney disease: distinct roles' J Immunol 179(11): 7466-7477.

2.?Liang, S. C., et al. (2003). 'Regulation of PD-1, PD-L1, and PD-L2 expression during normal and autoimmune responses' Eur J Immunol 33(10): 2706-2716.

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欣博盛生物是BioXcell 中國(guó)授權(quán)代理，Bioxcell國(guó)內(nèi)唯一現(xiàn)貨庫(kù)存中心，如需購(gòu)買產(chǎn)品或咨詢問題，請(qǐng)聯(lián)系。