不到3小時內(nèi)獲得!艾美捷支原體檢測試劑盒易于使用
艾美捷SouthernBiotech支原體檢測試劑盒提供了一種超靈敏、快速和簡單的熒光顯微鏡檢測方法,可用于在實驗室細胞培養(yǎng)物中目視識別支原體感染。要檢測支原體,可以將?SouthernBiotech熒光試劑直接加入到有細胞或無細胞的培養(yǎng)液中,染色后的樣品即可使用熒光顯微鏡觀察。
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艾美捷支原體檢測試劑盒中的PCR技術(shù)快速(結(jié)果通常在不到3小時內(nèi)獲得)且易于使用。工具包也高度敏感,可在100μL測試樣品上清液中檢測到低至2-5毫微克的支原體DNA。真核生物的并且來自細胞培養(yǎng)上清液的細菌DNA不被試劑盒擴增。感染支原體的樣本細胞系將在瓊脂糖凝膠上產(chǎn)生約448bp至約611bp的PCR產(chǎn)物,具體取決于支原體的類型。包括陽性對照(M.orale,503bp),以驗證PCR擴增過程具有以及確認在測試樣品中獲得的PCR產(chǎn)物的大小。還提供內(nèi)部控制以消除與PCR抑制劑相關(guān)的潛在假陰性。
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艾美捷支原體檢測試劑盒樣本數(shù)據(jù):
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