Product Details

The 407.6G12 monoclonal antibody reacts with phosphorylated mouse PD-1. Specifically, the antibody only reacts with PD-1 when tyrosine 248 (Y248) is phosphorylated. PD-1 is a 50-55 kDa cell surface receptor that belongs to the CD28 family of the Ig superfamily. PD-1 is transiently expressed on CD4 and CD8 thymocytes as well as activated T and B lymphocytes and myeloid cells. PD-1 expression declines after successful elimination of antigen. PD-1 signals via binding its two ligands, PD-L1 and PD-L2. Upon ligand binding, PD-1 signaling inhibits T-cell activation, leading to reduced proliferation, cytokine production, and T-cell death. Phosphorylation of Y248 of the ITSM motif of PD-1 is essential for the initiation of inhibitory signals. PD-1 blockade leads to reduced phosphor-PD-1. The 6G12 antibody can be used to assess the efficacy of PD-1 pathway blockade.

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BioXCell體內(nèi)MAb小鼠/人磷酸化PD-1(CD279)抗體：

407.6G12單克隆抗體與磷酸化小鼠PD-1反應(yīng)。具體來(lái)說，抗體僅在酪氨酸1（Y248）磷酸化時(shí)與PD-248反應(yīng)。PD-1是一種50-55 kDa的細(xì)胞表面受體，屬于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺細(xì)胞以及活化的T和B淋巴細(xì)胞和骨髓細(xì)胞上短暫表達(dá)。PD-1在成功消除抗原后表達(dá)下降。

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BioXCell艾美捷體內(nèi)MAb小鼠/人磷酸化PD-1(CD279)抗體規(guī)格：

# ?BXC-BE0387

Isotype?Mouse IgG2a, κ

Recommended Isotype Control(s)?InVivoMAb mouse IgG2a isotype control, unknown specificity

Recommended Dilution Buffer?InVivoPure pH 7.0 Dilution Buffer

Immunogen?Human PD-1 p248Tyr peptide conjugated to KLH

Reported Applications?Western Blot

Flow Cytometry

Formulation?PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin?<2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity?>95%

Determined by SDS-PAGE

Sterility?0.2 μm filtration

Production?Purified from tissue culture supernatant in an animal free facility

Purification?Protein G

Molecular Weight?150 kDa

Storage?The antibody solution should be stored at the stock concentration at 4°C.

同種型?小鼠IgG2a， κ

推薦的同種型對(duì)照?體內(nèi)MAb小鼠IgG2a同種型對(duì)照，特異性未知

推薦的稀釋緩沖液?體內(nèi)純?pH 7.0 稀釋緩沖液

免疫原?與KLH偶聯(lián)的人PD-1 p248Tyr肽

報(bào)告的應(yīng)用程序?蛋白質(zhì)印跡

流式細(xì)胞術(shù)

配方?PBS，pH 7.0

不含穩(wěn)定劑或防腐劑

內(nèi)毒素?<2EU/mg （<0.002EU/μg）

通過LAL凝膠凝血測(cè)定法測(cè)定

純度?>95%

由?SDS-PAGE 決定

無(wú)?菌?0.2 μm 過濾

生產(chǎn)?從無(wú)動(dòng)物設(shè)施中的組織培養(yǎng)上清液中純化

純化?蛋白?G

分子量?150 千達(dá)

存儲(chǔ)?抗體溶液應(yīng)以4°C的儲(chǔ)備濃度儲(chǔ)存。 不要冷凍。