FD NeuroApop? Kit is specifically designed for the detection of neuronal apoptosis in tissue sections from the central nervous system based on the principle of in situ DNA nick-end labeling (TUNEL) technique. The assay uses terminal deoxynucleotidyl transferase to catalyze the incorporation of biotinylated deoxyuridines onto the free 3′-hydroxyl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis. The integrated biotins are amplified and visualized with the avidin-biotin-complex (ABC) method4, enabling light microscopic identification.

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The reagents and procedure of FD NeuroApop? Kit have been optimized to achieve a high degree of both specificity and sensitivity for detecting apoptotic neurons with the lowest background. This kit can be used with frozen and paraffin sections, as well as cultured cells (cf. photo samples below). ?The procedure of the kit takes approximately 4 hours.

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Kit contents:

Part I (Store at -20°C)

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Digestive Enzyme ???????????????????????????????????2 ml x 4

Reaction Solution A ????????????????????????????????2 ml x 2

Reaction Solution B ????????????????????????????????85 μl

Reaction Solution C ????????????????????????????????60 μl

Chromogen Solution ???????????????????????????????20 ml

Part II (Store at 4°C)

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Equilibration Buffer ??????????????????????????????????20 ml

Detection Reagent ???????????????????????????????????5 ml

10x Phosphate-Buffered Saline ????????????250 ml x 2

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艾美捷FD NeuroTech?FD NeuroApap 神經(jīng)元TUNEL凋亡檢測試劑盒（#PK201）專為檢測中樞神經(jīng)系統(tǒng)組織切片中的神經(jīng)元凋亡而設(shè)計，基于原位DNA切口末端標記（TUNEL）技術(shù)的原理。該測定使用末端脫氧核苷酸轉(zhuǎn)移酶催化生物素化脫氧尿苷摻入DNA片段的游離3′-羥基末端，這被認為是細胞凋亡比較典型的特征之一?2, 3.整合的生物素通過親和素-生物素復(fù)合物（ABC）方法進行擴增和可視化4，可實現(xiàn)光學顯微鏡識別。

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FD NeuroApap 神經(jīng)元TUNEL凋亡檢測試劑盒（#PK201）的試劑和程序已經(jīng)過優(yōu)化，在檢測具有極低背景的凋亡神經(jīng)元時實現(xiàn)了高度的特異性和靈敏度。該試劑盒可用于冷凍和石蠟切片以及培養(yǎng)細胞（參見下面的照片樣品）。該套件的過程大約需要?4 小時。

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FD NeuroApap 神經(jīng)元TUNEL凋亡檢測試劑盒文獻引用：

1.Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.

2.Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6.

3.Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol. 136:593-608.

4.Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.

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