【標(biāo)題速讀】【Nprot】【2022年】【7-12月】
聲明：本專欄主要對(duì)生命科學(xué)領(lǐng)域的一些期刊文章標(biāo)題進(jìn)行翻譯，所有內(nèi)容均由本人手工整理翻譯。由于本人專業(yè)為生物分析相關(guān)，其他領(lǐng)域如果出現(xiàn)翻譯錯(cuò)誤請(qǐng)諒解。
功能定義的神經(jīng)元集合的靶向光刺激
The cover shows a strategy for ‘a(chǎn)ll-optical’ interrogation of neural circuits in vivo. Neuronal responses measured using two-photon calcium imaging were elicited by sequential, targeted two-photon photostimulation of 76 spatially clustered groups of neurons in mouse visual cortex in vivo. This large-scale but targeted activation is used to map the functional responses of neurons in the field of view for subsequent experiments. Responses are color-coded by the neurons targeted in a single group.
封面展示了一種在體內(nèi)“全光”詢問(wèn)神經(jīng)回路的策略。使用雙光子鈣成像測(cè)量的神經(jīng)元反應(yīng)是由體內(nèi)小鼠視覺(jué)皮層中76個(gè)空間聚集的神經(jīng)元組的連續(xù)、靶向雙光子光刺激引起的。 這種大規(guī)模但有針對(duì)性的激活用于繪制視野中神經(jīng)元的功能反應(yīng)，以供后續(xù)實(shí)驗(yàn)使用。響應(yīng)由單個(gè)組中的目標(biāo)神經(jīng)元進(jìn)行顏色編碼。
1.All-optical interrogation of neural circuits in behaving mice.
對(duì)行為小鼠的神經(jīng)回路進(jìn)行全光訊問(wèn)。
2.Hyperpolarized water as universal sensitivity booster in biomolecular NMR.
超極化水作為生物分子核磁共振的通用靈敏度增強(qiáng)劑。
3.Patient-derived and mouse endo-ectocervical organoid generation, genetic manipulation and applications to model infection.
患者衍生的和小鼠宮頸內(nèi)膜類器官的生成、遺傳操作和應(yīng)用于模型感染。
4.A puromycin-dependent activity-based sensing probe for histochemical staining of hydrogen peroxide in cells and animal tissues.
用于細(xì)胞和動(dòng)物組織中過(guò)氧化氫組織化學(xué)染色的基于嘌呤霉素的活性感應(yīng)探針。
5.The normative modeling framework for computational psychiatry.
計(jì)算精神病學(xué)的規(guī)范化建?？蚣堋?/p>
Adult Mnemiopsis leidyi in culture.
培養(yǎng)的成年雷氏蜉蝣。
1.Design and simulation of DNA, RNA and hybrid protein–nucleic acid nanostructures with oxView.
用oxView設(shè)計(jì)和模擬DNA、RNA和混合蛋白質(zhì)-核酸納米結(jié)構(gòu)。
2.Molecular profiling of enteric nervous system cell lineages.
腸道神經(jīng)系統(tǒng)細(xì)胞譜系的分子分析。
3.Formulation of two lipid-based membrane–core nanoparticles for FOLFOX combination therapy.
用于FOLFOX聯(lián)合治療的兩種脂質(zhì)膜芯納米顆粒的配制。
4.SubCellBarCode: integrated workflow for robust spatial proteomics by mass spectrometry.
SubCellBarCode：通過(guò)質(zhì)譜法進(jìn)行強(qiáng)大的空間蛋白質(zhì)組學(xué)研究的綜合工作流程。
5.Multigenerational laboratory culture of pelagic ctenophores and CRISPR–Cas9 genome editing in the lobate Mnemiopsis leidyi.
水層櫛水母的多代實(shí)驗(yàn)室培養(yǎng)和CRISPR-Cas9基因組編輯在葉狀的雷氏蜉蝣中的應(yīng)用。
A ten-day-old chicken embryo cleared using benzyl benzoate and imaged using a mesoSPIM light-sheet microscope with axially swept excitation. Neurofilament is shown in green and autofluorescence in magenta.
使用苯甲酸芐酯清理十天大的雞胚胎，并使用軸向掃描激發(fā)的mesoSPIM光片顯微鏡成像。 神經(jīng)絲顯示為綠色，自發(fā)熒光顯示為洋紅色。
1.An analytical workflow for dynamic characterization and quantification of metal-bearing nanomaterials in biological matrices.
生物基質(zhì)中含金屬的納米材料的動(dòng)態(tài)表征和定量的分析工作流程。
2.A practical guide to scanning light-field microscopy with digital adaptive optics.
數(shù)字自適應(yīng)光學(xué)掃描光場(chǎng)顯微鏡的實(shí)用指南。
3.Transcriptome-wide measurement of poly(A) tail length and composition at subnanogram total RNA sensitivity by PAIso-seq.
通過(guò)PAIso-seq在亞納克級(jí)總RNA靈敏度下進(jìn)行全轉(zhuǎn)錄組的聚(A)尾長(zhǎng)度和組成測(cè)量。
4.Practical and concise synthesis of nucleoside analogs.
核苷類似物的實(shí)用和簡(jiǎn)明的合成。
5.Isotropic imaging across spatial scales with axially swept light-sheet microscopy.
用軸向掃光片顯微鏡進(jìn)行跨空間尺度的各向同性成像。
6.A clinically relevant model of focal embolic cerebral ischemia by thrombus and thrombolysis in rhesus monkeys.
恒河猴血栓和溶栓的局灶性栓塞性腦缺血的臨床相關(guān)模型。
7.Screening circular RNAs with functional potential using the RfxCas13d/BSJ-gRNA system.
利用RfxCas13d/BSJ-gRNA系統(tǒng)篩選具有功能潛力的環(huán)狀RNA。
8.The cell-line-derived subcutaneous tumor model in preclinical cancer research.
細(xì)胞系衍生的皮下腫瘤模型在臨床前癌癥研究中的應(yīng)用。
Scanning electron microscope image (pseudo-color) of a fibroblast cell forming filopodia along nanofibers functionalized by the integrin-binding peptide Arg–Gly–Asp (RGD). The nanopatterns are arrays of 20-nm-wide lines with 80-nm center-to-center distance in each line pair. Functionalization of ligands on titanium nanopatterns enables super-resolution fluorescence microscopy to study cell–ligand interactions at the molecular scale.
成纖維細(xì)胞沿著由整合素結(jié)合肽Arg–Gly–Asp (RGD)功能化的納米纖維形成絲狀偽足的掃描電子顯微鏡圖像（偽彩色）。納米圖案是20納米寬的線陣列，每對(duì)線的中心距為80納米。鈦納米圖案上配體的功能化使超分辨率熒光顯微鏡能夠在分子尺度上研究細(xì)胞-配體相互作用。
1.Scalable tissue labeling and clearing of intact human organs.
完整人體器官的可擴(kuò)展的組織標(biāo)記和清除。
2.A rapid screening platform to coculture bacteria within tumor spheroids.
在腫瘤球狀體內(nèi)進(jìn)行細(xì)菌共培養(yǎng)的快速篩選平臺(tái)。
可以在3D多細(xì)胞細(xì)菌球體共培養(yǎng)（BSCC）系統(tǒng)中同時(shí)測(cè)試各種腫瘤和細(xì)菌細(xì)胞類型、基因回路和治療有效載荷。簡(jiǎn)而言之，腫瘤球體在96孔低粘附板中接種細(xì)菌，隨后洗滌和抗生素方案使球體內(nèi)的選擇性細(xì)菌生長(zhǎng)。共培養(yǎng)可穩(wěn)定≥2周，并與兩種細(xì)胞群動(dòng)力學(xué)研究的下游檢測(cè)兼容。候選細(xì)菌通過(guò)使用BSCC平臺(tái)快速迭代，并在小鼠腫瘤模型中進(jìn)行測(cè)試以進(jìn)行驗(yàn)證。
3.Nanocarriers based on bacterial membrane materials for cancer vaccine delivery.
基于細(xì)菌膜材料的納米載體用于癌癥疫苗的傳遞。
4.Ligand functionalization of titanium nanopattern enables the analysis of cell–ligand interactions by super-resolution microscopy.
鈦合金納米圖案的配體功能化使得超分辨率顯微鏡可以分析細(xì)胞與配體的相互作用。
5.High-throughput proteomic sample preparation using pressure cycling technology.
利用壓力循環(huán)技術(shù)的高通量蛋白質(zhì)組樣品制備。
6.I-TASSER-MTD: a deep-learning-based platform for multi-domain protein structure and function prediction.
I-TASSER-MTD：一個(gè)基于深度學(xué)習(xí)的多域蛋白質(zhì)結(jié)構(gòu)和功能預(yù)測(cè)平臺(tái)。
7.Single-cell RNA and protein profiling of immune cells from the mouse brain and its border tissues.
來(lái)自小鼠大腦及其邊界組織的免疫細(xì)胞的單細(xì)胞RNA和蛋白質(zhì)分析。
8.Single-crystal structure determination of nanosized metal–organic frameworks by three-dimensional electron diffraction.
三維電子衍射法測(cè)定納米金屬有機(jī)框架的單晶結(jié)構(gòu)。
Volcano plots are a unique tool that reveal the optimal homogeneous catalyst(s) for a chemical reaction, which appear at (or near) the volcano summit or plateau. In this protocol, we provide a detailed overview of how volcanoes can be constructed and interpreted from the results of electronic structure computations. A standalone code, volcanic, that automates construction is also presented.
火山圖是一種獨(dú)特的工具，可以揭示出現(xiàn)在火山頂峰或高原（或附近）的化學(xué)反應(yīng)的最佳均相催化劑。在此協(xié)議中，我們?cè)敿?xì)概述了如何根據(jù)電子結(jié)構(gòu)計(jì)算的結(jié)果構(gòu)建和解釋火山。還介紹了一個(gè)獨(dú)立的代碼，火山，可以自動(dòng)構(gòu)建。
1.Designing and executing prime editing experiments in mammalian cells.
在哺乳動(dòng)物細(xì)胞中設(shè)計(jì)和執(zhí)行先導(dǎo)編輯實(shí)驗(yàn)。
2.Small-animal blood exchange is an emerging approach for systemic aging research.
小動(dòng)物換血是系統(tǒng)性衰老研究的一種新興方法。
3.Synthesis of cyclodextrin derivatives for enantiodifferentiating photocyclodimerization of 2-anthracenecarboxylate.
環(huán)糊精衍生物的合成，用于2-蒽甲酸酯的對(duì)映分化光環(huán)二聚反應(yīng)。
4.Isolation of mitochondria-derived mitovesicles and subpopulations of microvesicles and exosomes from brain tissues.
從腦組織中分離線粒體衍生的線粒體和微泡亞群以及外泌體。
5.Constructing and interpreting volcano plots and activity maps to navigate homogeneous catalyst landscapes.
構(gòu)建和解釋火山圖和活動(dòng)圖，以瀏覽同質(zhì)催化劑的景觀。
6.Constructing a cost-efficient, high-throughput and high-quality single-molecule localization microscope for super-resolution imaging.
構(gòu)建低成本、高通量、高質(zhì)量的單分子定位顯微鏡，用于超分辨率成像。
7.Determining small-molecule permeation through lipid membranes.
確定小分子通過(guò)脂質(zhì)膜的滲透性。
8.Nacre-inspired underwater superoleophobic films with high transparency and mechanical robustness.
具有高透明度和機(jī)械堅(jiān)固性的珍珠層啟發(fā)的水下超疏散薄膜。
9.Principles of reproducible metabolite profiling of enriched lymphocytes in tumors and ascites from human ovarian cancer.
人類卵巢癌腫瘤和腹水中富集的淋巴細(xì)胞可重復(fù)代謝物分析的原則。
10.Directed differentiation of human pluripotent stem cells into diverse organ-specific mesenchyme of the digestive and respiratory systems.
人類多能干細(xì)胞定向分化為消化系統(tǒng)和呼吸系統(tǒng)的不同器官特異性間質(zhì)。
3D rendering of an eight-color confocal image of a human fetal kidney labeled for nephrogenic and structural markers using the multispectral large-scale single-cell resolution 3D (mLSR-3D) imaging protocol by van Ineveld, Collot and colleagues featured in this issue of Nature Protocols.
van Ineveld、Collot及其同事使用多光譜大尺度單細(xì)胞分辨率3D (mLSR-3D)成像方案繪制了人胎兒腎臟的八色共聚焦圖像的3D渲染圖，該方案在本期《自然方案》。
1.Establishment of human induced trophoblast stem cells via reprogramming of fibroblasts.
通過(guò)對(duì)成纖維細(xì)胞的重新編程建立人類誘導(dǎo)滋養(yǎng)細(xì)胞干細(xì)胞。
2.Induction of human trophoblast stem cells.
誘導(dǎo)人類滋養(yǎng)細(xì)胞干細(xì)胞。
3.Cultivation of previously uncultured microorganisms with a continuous-flow down-flow hanging sponge (DHS) bioreactor, using a syntrophic archaeon culture obtained from deep marine sediment as a case study.
用連續(xù)流下掛海綿（DHS）生物反應(yīng)器培養(yǎng)以前未培養(yǎng)的微生物，以從深海沉積物中獲得的合成古生物培養(yǎng)物為例進(jìn)行研究。
4.Metagenome analysis using the Kraken software suite.
使用Kraken軟件套件進(jìn)行元基因組分析。
5.Enzyme-free targeted DNA demethylation using CRISPR–dCas9-based steric hindrance to identify DNA methylation marks causal to altered gene expression.
使用基于CRISPR-dCas9的立體阻礙的無(wú)酶靶向DNA去甲基化，以確定對(duì)基因表達(dá)改變有因果關(guān)系的DNA甲基化標(biāo)記。
6.Measuring key human carbohydrate digestive enzyme activities using high-performance anion-exchange chromatography with pulsed amperometric detection.
利用高性能陰離子交換色譜法和脈沖安培檢測(cè)法測(cè)量關(guān)鍵的人類碳水化合物消化酶活性。
7.A microfluidic Braille valve platform for on-demand production, combinatorial screening and sorting of chemically distinct droplets.
用于按需生產(chǎn)、組合篩選和分揀化學(xué)性質(zhì)不同的液滴的微流控盲文閥平臺(tái)。
8.Neuroscience robotics for controlled induction and real-time assessment of hallucinations.
用于控制誘導(dǎo)和實(shí)時(shí)評(píng)估幻覺(jué)的神經(jīng)科學(xué)機(jī)器人技術(shù)。
9.MOF-derived nanoporous carbons with diverse tunable nanoarchitectures.
具有多種可調(diào)節(jié)納米結(jié)構(gòu)的MOF衍生的納米多孔碳。
10.Multispectral confocal 3D imaging of intact healthy and tumor tissue using mLSR-3D.
使用mLSR-3D對(duì)完整的健康和腫瘤組織進(jìn)行多光譜共聚焦三維成像。
11.Monitoring cell membrane recycling dynamics of proteins using whole-cell fluorescence recovery after photobleaching of pH-sensitive genetic tags.
利用pH值敏感的遺傳標(biāo)簽光漂白后的全細(xì)胞熒光恢復(fù)，監(jiān)測(cè)蛋白質(zhì)的細(xì)胞膜循環(huán)動(dòng)態(tài)。
