Human LR3 insulin-like Growth Factor-I (LR3IGF-I) is an 83 amino acid analogue of IGF-I comprising the complete human IGF-I sequence with the substitution of an Arginine for the Glutamine at position 3,plus a 13 amino acid extension peptide at the N-terminus.

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Human LR3IGF-I is more potent than IGF-I in vitro and in vivo. This increased potency is due to reduced binding of LR3IGF-I to most of the IGF binding proteins which modify the biological actions of IGF-I.

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Human LR3IGF-I binds to the type 1 IGF receptor with similar affinity to wild type IGF-I.LR3

IGF-I was developed by GroPep specifically for supplementation of mammalian cell culture to support the survival and proliferation of cells. It is engineered to have a higher biological potency than native IGF-I or IGF-II and has several advantages over recombinant insulin. Supplementation of cell cultures with LR3IGF-I at a much lower concentration results in equivalent or better productivity than supplementation with standard concentrations of insulin. LR3IGF-I is better able to stimulate the type I IGF receptor and thus induce a higher level of activation of intracellular signalling molecules which are responsible for promoting cell survival by inhibition of apoptosis.

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Media grade LR3IGF-I is a high quality product for use in commercial cell culture.It can also be used as a research reagent at an economical cost to enable studies where higher quantities of peptide are required.

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References Francis GL et al (1992) Journal of Molecular Endocrinology 8, 213-223

Yandell C et al (2004) Bioprocess International 2, 56-64

Source: Produced in E.coli.

Molecular Weight: 9110.6 ± 2 Daltons

Purity: ≥ 95 % (by SDS gel electrophoresis)

HPLC analysis: Three peaks with main peak being > 50% of total area

(Other species are micro heterogeneous forms which have biological activity)

N-terminal sequence: Analysis of 18 residues

Biological Activity: Proliferation of Chinese Hamster Ovary (CHO) cells

(EC50 ≤ 10 ng/ml)

Endotoxin: ≤ 0.1 EU/μg

Appearance: White powder freeze-dried from 0.1M acetic acid and stored under

nitrogen at a slight vacuum.

Storage/Stability: At least 2 years at 2-8°C (as a freeze dried product).

Reconstitution: Handling of GroPep IGF-I, IGF-II and IGF analogues

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艾美捷LR3胰島素樣生長(zhǎng)因子-I#GRO-AM001是IGF-I的83個(gè)氨基酸的類似物，其包含在位置3用精氨酸取代谷氨酰胺的完整人IGF-I序列，加上在N-末端的13個(gè)氨基酸的延伸肽。

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人類LR3 IGF-I在體外和體內(nèi)都比IGF-I更有效。這種效力的提高是由于LR3的結(jié)合IGF-I與大多數(shù)改變IGF-I生物作用的IGF結(jié)合蛋白結(jié)合。

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人類LR3 IGF-I以與野生型IGF-I相似的親和力與1型IGF受體結(jié)合。LR3型IGF-I由GroPep開發(fā)，專門用于補(bǔ)充哺乳動(dòng)物細(xì)胞培養(yǎng)物支持細(xì)胞的存活和增殖。它被設(shè)計(jì)成具有比天然IGF-I或IGF-II，并且與重組胰島素相比具有幾個(gè)優(yōu)點(diǎn)。細(xì)胞的補(bǔ)充含有LR3的培養(yǎng)物IGF-I在低得多的濃度下產(chǎn)生與補(bǔ)充標(biāo)準(zhǔn)濃度的胰島素。LR3型IGF-I能夠更好地刺激I IGF受體，從而誘導(dǎo)更高水平的細(xì)胞內(nèi)信號(hào)分子激活負(fù)責(zé)通過(guò)抑制細(xì)胞凋亡來(lái)促進(jìn)細(xì)胞存活。

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LR3胰島素樣生長(zhǎng)因子-I詳細(xì)信息：

來(lái)源：產(chǎn)自大腸桿菌。

分子量：9110.6±2道爾頓

純度：≥95%（SDS凝膠電泳）

HPLC分析：三個(gè)峰，主峰面積大于總面積的50%

（其他物種是具有生物活性的微異質(zhì)形式）

N端序列：18個(gè)殘基的分析

生物活性：中國(guó)倉(cāng)鼠卵巢（CHO）細(xì)胞的增殖

（EC50≤10 ng/ml）

內(nèi)毒素：≤0.1 EU/μg

外觀：從0.1M乙酸中冷凍干燥的白色粉末，在

在輕微真空下的氮?dú)狻?/p>

儲(chǔ)存/穩(wěn)定性：在2-8°C下至少保存2年（作為冷凍干燥產(chǎn)品）。

重組：GroPep IGF-I、IGF-II和IGF類似物的處理

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LR3胰島素樣生長(zhǎng)因子-I相關(guān)研究：

人LR3IGF-I（受體級(jí)）

人[Arg3]IGF-I（介質(zhì)級(jí)）

人LR3IGF-I ELISA

人IGF-I（介質(zhì)級(jí)）

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文獻(xiàn)參考：

Francis GL et al (1992) Journal of Molecular Endocrinology 8, 213-223?Yandell C et al (2004) Bioprocess International 2, 56-64??