該DNA分析試劑盒是利用RT-PCR方法，以核DNA (nDNA) 為基準對線粒體DNA (mtDNA) 拷貝數(shù)進行相對定量檢測?？捎糜跈z測人、大鼠或小鼠樣本。

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試劑盒主要成分：

●?96孔PCR板

●?RT-PCR反應混合物?

●?線粒體DNA（mtDNA）引物

●?核DNA（nDNA）引物

●?陽性對照

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?正常人與糖尿病患者視網(wǎng)膜mtDNA拷貝數(shù)的比較

Santos et.al. Free Radical Biology & Medicine 51: 1849-1860, 2011.

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左：大鼠陽性對照RT-PCR擴增曲線 ????????????右：mtDNA的RT-PCR分析

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產(chǎn)品信息

名稱????????????????????????????貨號????????????????規(guī)格

Human Mitochondrial Copy Number Assay? ?????MCN1????????44?reactions

Rat Mitochondrial Copy Number Assay? ????????????MCN2????????44?reactions

Mouse Mitochondrial Copy Number Assay????????MCN3????????44?reactions

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近期參考文獻

1.Singh, K. et al. (2020) Developmental regression and mitochondrial function in children with autism.?Annals of Clinical and Translational Neurology 7:?683-694.?

Mishra, J.S. et al. (2020) Testosterone decreases placental mitochondrial content and cellular bioenergetics. Biology 8L176;

Guo, X. et al. (2020) STAMP: a multiplex method for simultaneous evaluation of mitochondrial DNA heteroplasmies and content.

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