PCR Array應用場景：轉錄組測序驗證

文章主要方法：

1. Transcriptome sequencing
   轉錄組高通量測序

2. PCR Array (Wcgene Biotech, Shanghai, China)
   沃吉基因定制化PCR Array

3. CRISPR/Cas9-targeted bmp6 gene knockout
   CRISPR/cas9靶向bmp6基因敲除
   

文章結果：

通過轉錄組分析篩選了許多同源的IBs調控基因

沃吉基因定制化PCR Array進一步驗證分析IBs相關基因

文章來源：

Zheng J, He C, Jiang W, et al. Screening for IBs-relative genes by transcriptome analysis and generation IBs-less mutants in Culter alburnus[J]. Comparative Biochemistry and Physiology Part D: Genomics and Proteomics, 2023: 101106.