艾美捷牛粒細(xì)胞-巨噬細(xì)胞集落刺激因子/Bovine GM-CSF重組蛋白中文說(shuō)明書(shū)：

目錄編號(hào)（規(guī)格）：

RP0871B-005（5μg）

RP0871B-025（25μg）

RP0871B-100（100μg）

來(lái)源：酵母

別名：CSF-2

配方：無(wú)載體蛋白冷凍。

重建：用至少含有磷酸鹽的無(wú)菌緩沖鹽水重建

0.1%載體蛋白。

穩(wěn)定性和儲(chǔ)存：在-20°C溫度下，自收到之日起可穩(wěn)定12個(gè)月。在工作等分試樣中儲(chǔ)存時(shí)至少可穩(wěn)定3個(gè)月在-20°C下具有載體蛋白。避免反復(fù)凍融循環(huán)。

分子量：14.3 kDa（計(jì)算）

生物活性：重組牛GM-CSF的生物活性為在使用人TF-1的細(xì)胞增殖測(cè)定中測(cè)量細(xì)胞系。這種效應(yīng)的ED50通常為2.5-4.0 ng/mL。

純度：通過(guò)SDS-PAGE分析顯示>95%。

純化：離子交換色譜法

Entrez基因ID:281095

氨基酸序列：APTRPNTAT RPWQHVDAIK EALSLLNHSS DTDAVNDTE VVSEKFDSQE ptclctrlkl YKNGLQGSLT SLMGSLTMMA

氨基酸同源性：牛頭（牛）GM-CSF–100%

Bos mutus（野牦牛）GM-CSF–99%

野牛野牛（野牛）GM-CSF–98%

印度博斯（澤布）GM-CSF–98%

原產(chǎn)國(guó)：美國(guó)

牛粒細(xì)胞-巨噬細(xì)胞集落刺激因子/Bovine GM-CSF重組蛋白英文說(shuō)明書(shū)：

Catalog Number (Quantity): RP0871B-005 (5 μg)

RP0871B-025 (25 μg)

RP0871B-100 (100 μg)

Source: Yeast

Alias: CSF-2

Formulation: Lyophilized without carrier protein.

Reconstitution: Reconstitute with sterile phosphatebuffered saline containing at least

0.1% carrier protein.

?01/18/2019

Stability and Storage: Stable for up to twelve months from date of receipt at -20°C.

Stable for at least 3 months when stored in working aliquots

with a carrier protein at -20°C. Avoid repeated freeze/thaw

cycles.

Molecular Weight: 14.3 kDa (calculated)

Bioactivity: The biological activity of recombinant bovine GM-CSF was

measured in a cell proliferation assay using the human TF-1

cell line. The ED50 for this effect is typically 2.5 - 4.0 ng/mL.

Purity: >95% as visualized by SDS-PAGE analysis.

Purification: Ion-exchange chromatography

Entrez Gene ID: 281095

Amino Acid Sequence: APTRPPNTAT RPWQHVDAIK EALSLLNHSS DTDAVMNDTE

VVSEKFDSQE PTCLQTRLKL YKNGLQGSLT SLMGSLTMMA

THYEKHCPPT PETSCGTQFI SFKNFKEDLK EFLFIIPFDC

WEPAQK (126)

Amino Acid Homology: Bos taurus (cattle) GM-CSF – 100%

Bos mutus (wild yak) GM-CSF – 99%

Bison bison bison (bison) GM-CSF – 98%

Bos indicus (zebu) GM-CSF – 98%

Country of Origin: USA

Warranty: Products are warranted by Kingfisher Biotech, Inc. to meet stated product

specifications and to conform to label descriptions when used, handled and

stored according to instructions. Unless otherwise stated, this warranty is

limited to one year from date of sale or expiry date, whichever comes first.

Kingfisher Biotech’s sole liability for the product is limited to replacement of

the product or refund of the purchase price. Kingfisher Biotech products are

supplied for research applications. They are not intended for medicinal,

diagnostic or therapeutic use.