艾美捷CY7標(biāo)記鏈霉親和素的功能和應(yīng)用價(jià)值
鏈霉親和素與生物素(Biotin)有很高的結(jié)合活性,因此被廣泛用于檢測(cè)生物素或生物素偶聯(lián)的各種蛋白、核酸以及其它分子。
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艾美捷Cy7標(biāo)記鏈霉親和素包括鏈親和蛋白(作為生物素結(jié)合蛋白),Cy7共價(jià)連接(作為熒光標(biāo)記)。該Cy7標(biāo)記鏈霉親和素是使用AAT Bioquest的特別標(biāo)記技術(shù)制備的,可以顯示出更亮的信號(hào),因此可以顯著提高測(cè)定靈敏度。當(dāng)與生物素化的一級(jí)抗體結(jié)合使用時(shí),它通常用作間接免疫熒光染色的第二步試劑。它是一種有價(jià)值的工具,用于基于生物素-鏈霉親和素的生物測(cè)定和測(cè)試,使用帶有Cy7過(guò)濾器的流式細(xì)胞術(shù)。
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艾美捷CY7標(biāo)記鏈霉親和素基本參數(shù):
中文名稱:Cy7標(biāo)記鏈霉親和素
英文名字:Cy7-streptavidin conjugate
編號(hào):AAT-16914
規(guī)格:1mg
描述:Cy7標(biāo)記的鏈霉親和素的光譜學(xué)相關(guān)參數(shù)為Ex=749nm/ Em=776nm;
形式:固體,推薦將其溶解于水中使用或保存。
保存建議:推薦室溫運(yùn)輸。建議在低于-20°避光保存,有效期12個(gè)月。

圖.用(紅色,+)或不使用(綠色,-)小鼠抗人HLA-ABC生物素(W6/32生物素)孵育HL-60細(xì)胞,然后用Cy7?-鏈霉親和素綴合物孵育。在APC-C7通道中使用ACEA NovoCyte流式細(xì)胞儀監(jiān)測(cè)熒光信號(hào)。
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艾美捷CY7標(biāo)記鏈霉親和素文獻(xiàn)引用:
Persistent repression of tau in the brain using engineered zinc finger protein transcription factors
Authors: Wegmann, Susanne and DeVos, Sarah L and Zeitler, Bryan and Marlen, Kimberly and Bennett, Rachel E and Perez-Rando, Marta and MacKenzie, Danny and Yu, Qi and Commins, Caitlin and Bannon, Riley N and others,
Journal: Science Advances (2021): eabe1611
Overexpression of MACC1 and the association with hepatocyte growth factor/c-Met in epithelial ovarian cancer
Authors: Li, Hongyu and Zhang, Hui and Zhao, Shujun and Shi, Yun and Yao, Junge and Zhang, Yanyan and Guo, Huanhuan and Liu, Xingsuo
Journal: Oncology letters (2015): 1989--1996
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艾美捷CY7標(biāo)記鏈霉親和素參考文獻(xiàn):
A streptavidin paramagnetic-particle based competition assay for the evaluation of the optical selectivity of quadruplex nucleic acid fluorescent probes
Authors: Largy E, Hamon F, Teulade-Fichou MP.
Journal: Methods. (2012)
Biotin-4-fluorescein based fluorescence quenching assay for determination of biotin binding capacity of streptavidin conjugated quantum dots
Authors: Mittal R, Bruchez MP.
Journal: Bioconjug Chem (2011): 362
Iminobiotin binding induces large fluorescent enhancements in avidin and streptavidin fluorescent conjugates and exhibits diverging pH-dependent binding affinities
Authors: Raphael MP, Rappole CA, Kurihara LK, Christodoulides JA, Qadri SN, Byers JM.
Journal: J Fluoresc (2011): 647