熒光素二-β-D-半乳糖苷 (FDG)
Fluorescein di-?-D-galactopyranoside (熒光素二-β-D-半乳糖苷,FDG) 用于體內(nèi)和體外 lacZ ?-半乳糖苷酶檢測(cè)的超靈敏底物。fluorescein di-?-D-galactopyranoside (FDG)廣泛用于通過流式細(xì)胞術(shù)檢測(cè)哺乳動(dòng)物細(xì)胞和革蘭氏陰性菌中的大腸桿菌 lacZ β-半乳糖苷酶。 進(jìn)入活細(xì)胞。 與 lacZ酶活性 成比例的熒光信號(hào)。
溶解度:DMSO: soluble
性狀:Solid
儲(chǔ)藏條件:Storage conditionsstorage at -4℃ (1-2weeks), longer storage period at -20℃ (1-2years)
Sternomastoid muscle fibers of a living mouse. YOYO-1 and fluorescein di-beta-D-galactopyranoside (FDG).Sternomastoid muscle fibers of a living mouse that have been transfected with YOYO-1 dye–stained DNA (red) containing the <i>lacZ</i> reporter gene and then stained with the beta-galactosidase substrate fluorescein di-beta-D-galactopyranoside (FDG, F1179). DNA stained with YOYO-1 (Y3601) prior to implantation could still be localized five days after application. Fluorescence signals were visualized <i>in situ</i> by epifluorescence microscopy with a low–light level SIT camera and a computer imaging system. Image contributed by Peter van Mier, Washington University School of Medicine.
文獻(xiàn):
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