貨號(hào)：RVV02823

產(chǎn)品鏈接：http://www.antibodysystem.com/product/14140.html

物種反應(yīng)性：Human respiratory syncytial virus A (strain A2)

形式：Liquid

存儲(chǔ)緩沖區(qū)：0.01M PBS, pH 7.4.

濃度：1 mg/ml

純度：>95% by SDS-PAGE.

克隆性：Monoclonal

應(yīng)用：ELISA

儲(chǔ)存：Use a manual defrost freezer and avoid repeated freeze-thaw cycles.Store at +4°C short term (1-2 weeks).Store at -20 °C 12 months. Store at -80°C long term.

參考文獻(xiàn)：

Identification of subgroups of bovine respiratory syncytial virus. PMID: 1583108
Infectivity of a human respiratory syncytial virus lacking the SH, G, and F proteins is efficiently mediated by the vesicular stomatitis virus G protein. PMID: 12610153

Transmembrane Domain Dissociation Is Required for Hendra Virus F Protein Fusogenic Activity. PMID: 31462574
Development of liposomes and pseudovirions with fusion activity for efficient gene delivery. PMID: 22023472
Molecular Features of the Measles Virus Viral Fusion Complex That Favor Infection and Spread in the Brain. PMID: 34061592
Mutations in the parainfluenza virus 5 fusion protein reveal domains important for fusion triggering and metastability. PMID: 24089572
The electrophysiological net response ('F-complex') to spatial fusion of speech elements forming an auditory object. PMID: 12738428

HRSV基因組全長(zhǎng)約15.2 kb，按照3′到5′順序含有10個(gè)基因，分別為3′ -leader-NS1-NS2-N-P-M-SH-G-F-M2-L-trailer-5′，共編碼11種蛋白，包括2個(gè)非結(jié)構(gòu)蛋白及9個(gè)結(jié)構(gòu)蛋白。NS1、NS2是非結(jié)構(gòu)蛋白，其主要功能是抑制干擾素的合成。核衣殼蛋白（N）與磷蛋白（P）為病毒核酸合成提供場(chǎng)所，多聚酶亞單位蛋白（L）是病毒核酸轉(zhuǎn)錄和復(fù)制的必需成分?；|(zhì)蛋白（M）是內(nèi)包膜蛋白，可以有效抑制宿主細(xì)胞轉(zhuǎn)錄。M2-1為核蛋白相關(guān)蛋白，所包含的轉(zhuǎn)錄因子與病毒轉(zhuǎn)錄有關(guān)。M2下游開放閱讀框編碼M2-2調(diào)控蛋白，負(fù)責(zé)病毒粒子組裝過程中RNA合成；其中小疏水（SH）、附著（G）和融合（F）基因編碼病毒的3種表面蛋白，其中G、F蛋白是HRSV兩個(gè)重要膜表面糖蛋白，刺激機(jī)體產(chǎn)生中和抗體，G蛋白輔助病毒黏附于宿主細(xì)胞，F(xiàn)蛋白負(fù)責(zé)病毒融合和合胞體形成，而SH蛋白功能尚不清楚，可能與病毒感染有關(guān)。