艾美捷人CD25 ELISA試劑盒的應(yīng)用和樣品類型介紹
CD25是異三聚體IL-2受體的α鏈,在許多類型的血液系統(tǒng)惡性腫瘤中高水平表達(dá),但在大多數(shù)實(shí)體腫瘤中低水平表達(dá)。CD25也高度表達(dá)于活化的循環(huán)免疫細(xì)胞和調(diào)節(jié)性T細(xì)胞(Tregs)。Tregs在腫瘤微環(huán)境中的浸潤(rùn)可導(dǎo)致效應(yīng)T細(xì)胞(Teffs)和Tregs的比例失衡,這與腫瘤的進(jìn)展相關(guān)?;貧w平衡的Teff/Treg細(xì)胞比例則有利于提升免疫治療的有效性。
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CD25作為Tregs耗竭的潛在靶點(diǎn)對(duì)于開發(fā)新的免疫治療策略至關(guān)重要。目前很少有文章總結(jié)了CD25與腫瘤之間的關(guān)系,或靶向CD25藥物的最新進(jìn)展。
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Covalab ELISA試劑盒包括預(yù)涂條形板、生物素化第二抗體、標(biāo)準(zhǔn)品、對(duì)照品(如適用)、緩沖液、鏈親和素-HRP、TMB、終止試劑和詳細(xì)程序。
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艾美捷人CD25 ELISA試劑盒詳細(xì)信息:
ID Covalab:kit30161
應(yīng)用:ELISA
活性:交叉反應(yīng)與天然和重組人可溶性IL-2R反應(yīng)。不會(huì)與其他人類可溶性受體發(fā)生交叉反應(yīng)。
物種:Hu
樣品類型:血清、血漿、細(xì)胞培養(yǎng)上清液
含量測(cè)定范圍:68.75-2220 pg/ml
測(cè)定持續(xù)時(shí)間:3h40
預(yù)涂模板
靶向CD25/IL-2R?
產(chǎn)品類型:kit
序列:68.75-2220 pg/ml
工作稀釋液:8 pg/ml
靈敏度:8 pg/ml
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Covalab ELISA Kits include pre-coated strip plates, biotinylated secondary antibody, standards, controls (where applicable), buffers, streptavidin-HRP, TMB, stop reagents, and a detailed procedure.
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More Information
ID Covalab?kit30161
Applications?ELISA
Activity cross reaction?Reacts with both natural and recombinant human soluble IL-2R. Does not cross-react with other human soluble receptors.
Species?Hu
Sample type?Serum, Plasma, Cell culture supernatant
Assay range?68.75-2220 pg/ml
Assay duration?3h40
Form?Pre-coated
Target?CD25 / IL-2R
Product type?Kits
Sequence?68.75-2220 pg/ml
Working dilutions?8 pg/ml
Sensitivity?8 pg/ml