ANS-Na,疏水牛血清白蛋白熒光探針
2022-11-06 09:37 作者:BIOFOUNT范德生物 | 我要投稿
ANS-Na (8-苯胺基-1-萘磺酸鈉) 是一種疏水熒光染料,ANS 用于研究蛋白折疊/去折疊途徑的中間狀態(tài)的重要染料。也可用于檢測(cè)與蛋白聚集有關(guān)的構(gòu)象變化。ANS在水溶液中是非熒光的,但與BSA(牛血清白蛋白)和變性蛋白結(jié)合后發(fā)出強(qiáng)烈熒光。它可用于檢測(cè)血清白蛋白。ANS主要與暴露于溶劑的非極性(疏水性)位點(diǎn)相互作用。
溶解度Solubility溶于水
性狀Solid,power
儲(chǔ)藏條件 Storage conditions4℃條件下存儲(chǔ),-4℃存儲(chǔ)更佳
PH 對(duì)ANS探針的影響:
(A) Effect of pH on the binding of 8-anilino-1-naphthalenesulfonic acid (ANS) with CdGSTM1. The binding of ANS (200 μM) with CdGSTM1 (4 μM) at pH 1.0-10.0 was monitored by excitation at 380 nm (bandwidth 2.5 nm) and emission spectra collected from 400-600 nm (bandwidth 5 nm) at 22?C. (B) Rayleigh light scattering measurements of CdGSTM1 (0.1 mg mL-1 ) were obtained at 500 nm wavelength at pH 1.0-10.0.
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