PerCP蛋白（AAT-2559）英文說(shuō)明：

PerCP (Peridinin-chlorophyll-protein complex) is isolated from Dinophyceae sp. It has an extremely high extinction coefficient, a high quantum efficiency and a large Stokes shift. It is well excited with the Argon laser at 488 nm with its maximum emission peak at 677nm. PerCP protein is commonly used for fluorescent immunolabeling, particularly in applications involving fluorescent-activated cell sorting (FACS). Its cyanine tandem conjugates (such as PerCP-Cy5.5 developed by BD) can be excited with a standard 488 nm laser and emits in the far red at a longer wavelength for multicolor flow cytometric analysis of cells. These multiple emission wavelengths make PerCP- Cyanine conjugates potentially useful fluorochromes for multicolor analysis with FITC, PE and other fluorochromes. PerCP tandem structure may make it more photostable than PerCP alone, which generally photobleaches rapidly with more powerful water-cooled gas lasers. AAT Bioquest offers iFluor protein labeling dyes that are generally brighter and more photostable than the corresponding cyanine dyes of similar wavelengths. iFluor protein labeling dyes are superior alternatives to the cyanine dyes.

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PerCP蛋白（AAT-2559）艾美捷中文說(shuō)明：

PerCP（Peridinin葉綠素蛋白復(fù)合物）是從Dinophyceae sp。中分離出來(lái)的。它具有極高的消光系數(shù)，高量子效率和大的斯托克斯位移。它在488 nm處被氬激光很好地激發(fā)，其最大發(fā)射峰在677nm處。PerCP蛋白通常用于熒光免疫標(biāo)記，特別是在涉及熒光激活細(xì)胞分選（FACS）的應(yīng)用中。它的花青串聯(lián)共軛物（例如BD開(kāi)發(fā)的PerCP-Cy5.5）可以用標(biāo)準(zhǔn)的488 nm激光激發(fā)，并以更長(zhǎng)的波長(zhǎng)發(fā)射遠(yuǎn)紅色，用于細(xì)胞的多色流式細(xì)胞儀分析。這些多發(fā)射波長(zhǎng)使得PerCP-Cyanine共軛物可能是有用的熒光染料，用于FITC，PE和其他熒光染料的多色分析。PerCP串聯(lián)結(jié)構(gòu)可以使其比單獨(dú)的PerCP更具光穩(wěn)定性，PerCP通常使用更強(qiáng)大的水冷氣體激光器快速光漂白。AAT?iFluor蛋白標(biāo)記染料通常比相應(yīng)波長(zhǎng)相似的花青染料更亮，更具光穩(wěn)定性。iFluor蛋白標(biāo)記染料是花青染料的優(yōu)良替代品。

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PerCP蛋白基本參數(shù)：

中文名稱：PerCP(多甲藻黃素-葉綠素-蛋白質(zhì) 復(fù)合物)

英文名字：PerCP(Peridinin-Chlorophyll-Protein Complex)

規(guī)格：1mg

形式：固體形式

保存建議：在低于2-4℃溫度下冷藏保存，要求儲(chǔ)存環(huán)境干燥并避免光照

分子量35000

溶劑水

冷藏（2-8°C）；盡量減少曝光

圖：Peridinin葉綠素（PerCP）是一些生物在光合作用中使用的類胡蘿卜素色素。許多光合作用的甲藻使用peridinin，其吸收470-550nm范圍內(nèi)的藍(lán)綠光，超出葉綠素分子可接近的范圍。

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艾美捷peridinin-葉綠素-蛋白質(zhì)復(fù)合物是一種特殊的分子復(fù)合物，由船形蛋白質(zhì)分子組成，具有大的中心腔，含有peridinin，葉綠素和脂質(zhì)分子，通常以peridinin與葉綠素的4:1比例。PerCP通常用于免疫測(cè)定，例如熒光激活細(xì)胞分選（FACS）和流式細(xì)胞術(shù)。熒光團(tuán)與用于研究應(yīng)用的蛋白質(zhì)或抗體共價(jià)連接。

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艾美捷PerCP蛋白相關(guān)文獻(xiàn)：

Phycobiliprotein-Fab conjugates as probes for single particle fluorescence imaging

Authors: Triantafilou K, Triantafilou M, Wilson KM.

Journal: Cytometry (2000): 226

Novel activity of a phycobiliprotein lyase: both the attachment of phycocyanobilin and the isomerization to phycoviolobilin are catalyzed by the proteins PecE and PecF encoded by the phycoerythrocyanin operon

Authors: Zhao KH, Deng MG, Zheng M, Zhou M, Parbel A, Storf M, Meyer M, Strohmann B, Scheer H.

Journal: FEBS Lett (2000): 9

Phycobiliprotein and fluorescence immunological assay

Authors: Wu P., undefined

Journal: Sheng Li Ke Xue Jin Zhan (2000): 82