磷酸根熒光探針-BIOFOUNT
磷酸根熒光探針,該產(chǎn)品是一種雙發(fā)色團(tuán)熒光激光染料,主要用于有機(jī)農(nóng)藥中的磷酸根離子檢測,在420 nm-520nm下有激光作用。
溶解度:溶于醇,丙酮,乙醚,甲醇
性狀:White to Amber to Dark green powder to crystal
儲藏條件 Storage conditionsStore at 4°C,-4℃下存儲更優(yōu).
圖1:Fluorescence ofcoumarin1 (C1) at p-nitrophenol (pNP) concentrations from 0.4× 10 ?6 to 0.173 × 10 ?3 M. (i) C1, (ii) C1 +WT, (iii) 0.4M, (iv) 0.7 M,
(v) 1.8 M, (vi) 3.5 M, (vii) 7 M, (viii) 18 M, (ix) 35 M, (x) 87 M, and (xi) 173 M. (b) Calibration curve with p-nitrophenol. Relative fluorescence intensity?change (RFI) is plotted as a function of added p-nitrophenol (pNP) concentration. (c) Fluorescence of Coumarin1 at p-nitrophenol (pNP) concentrations from?0.4× 10 ?6 to 0.173 × 10 ?3 M in absence of OPH; (i) C1, (ii) 0.4M, (iii) 0.7 M, (iv) 1.8 M, (v) 3.5 M, (vi) 7 M, (vii) 18 M, (viii) 35 M, (ix) 87 M, and?(x) 173 M. All the experiments were conducted in the absence/presence of OPH in 20mM CHES buffer, pH 9.0. [Coumarin1] =2.33 × 10 ?8 M. Coumarin1 was?excited at 343 nm. Coumarin1: OPH= 1:1.