P-6002-10PK，P-6002-2PK脂質(zhì)研究工具解析
P-6002-10PK，P-6002-2PK脂質(zhì)研究工具——艾美捷膜脂試紙條。一起來(lái)看看，如何快速篩選和鑒定，目標(biāo)蛋白與哪類膜脂會(huì)結(jié)合并產(chǎn)生？
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艾美捷?P-6002-10PK，P-6002-2PK Membrane Lipid Strips是一種2 * 6 cm的疏水膜試紙條，包含了細(xì)胞膜中常見(jiàn)的15種具有不同生物功能的重要脂質(zhì)，每種脂類含量均為100 pmol。通過(guò)一個(gè)簡(jiǎn)單的蛋白質(zhì)-脂質(zhì)覆蓋實(shí)驗(yàn)（PLO）來(lái)確定脂質(zhì)-蛋白質(zhì)之間的相互作用。這為研究人員提供了一種方便的方法來(lái)確定他們感興趣的蛋白質(zhì)是否與一種或多個(gè)結(jié)合的脂質(zhì)相互作用。
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艾美捷?P-6002-10PK，P-6002-2PK已發(fā)表高分認(rèn)證文章——Cell, Nature（見(jiàn)文末）斑點(diǎn)分布如下：
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艾美捷?P-6002-10PK，P-6002-2PK相關(guān)脂類研究：
脂類英文?中文
Glyceryl tripalmitate or Triglyceride (GT)?甘油三酯（GT）
Diacylglycerol (DAG, L-0016)?甘油二脂(DAG, L-0016)
Phosphatidic Acid (PA, L-4116)?磷脂酸?(PA, L-4116)
Phosphatidylserine (PS, L-3116)?磷脂酰絲氨酸?(PS, L-3116)
Phosphatidylethanolamine (PE, L-2116)?磷脂酰乙醇胺?(PE, L-2116)
Phosphatidylcholine (PC, L-1116)?磷脂酰膽堿?(PC, L-1116)
Phosphatidylglycerol (PG, L-5116)?磷脂酰甘油?(PG, L-5116)
Cardiolipin (CL, L-C160)?心磷脂?(CL, L-C160)
Phosphatidylinositol (PI P-0016)?磷脂酰肌醇?(PI P-0016)
PtdIns(4)P (P-4016)?磷脂酰肌醇(4)-磷酸?(P-4016)
PtdIns(4,5)P2 (P-4516)?磷脂酰肌醇(4,5)-二磷酸?(P-4516)
PtdIns(3,4,5)P3 (P-3916)?磷脂酰肌醇(3,4,5)-三磷酸酯?(P-3916)
Cholesterol (L-6012)?膽固醇?(L-6012)
Sphingomyelin (SM)?鞘磷脂?(SM)
Sulfatides?硫酸腦苷脂
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艾美捷?P-6002-10PK，P-6002-2PK Nature/Cell文章鑒賞：
①Nature文章：Liu, Xing, et al. "Inflammasome-activated gasdermin D causes pyroptosis by forming membrane pores." Nature 535.7610 (2016): 153-158.
②?Cell文章：Liao, Ya-Cheng, et al. "RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether." Cell 179.1 (2019): 147-164.
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Nature/Cell?已發(fā)表文章（部分）：
Lee, Jaewoong, et al. "IFITM3 functions as a PIP3 scaffold to amplify PI3K signalling in B cells." Nature 588.7838 (2020): 491-497.
Uzureau, Sophie, et al. "APOL1 C-terminal variants may trigger kidney disease through interference with APOL3 control of actomyosin." Cell reports30.11 (2020): 3821-3836.
Suarez, Catherine, et al. "A lipid-binding protein mediates rhoptry discharge and invasion in PlaSModium falciparum and ToxoplaSMa gondii parasites." Nature communications10.1(2019): 1-14.
Liao, Ya-Cheng, et al. "RNA granules hitchhike on lysosomes for long-distance transport, using annexin A11 as a molecular tether." Cell 179.1(2019): 147-164.
