今天，請大家一起來看看基礎醫(yī)學SCI文章中實驗設計、方法、結果以及圖表等方面的常見修回意見，了解審稿人關注的點，盡量在寫作中提前規(guī)避掉這些問題，可大大提高文章發(fā)表的速度哦。

實驗設計

When testing a compound, drug or some other chemical or physical agents on cell lines, it is important for our readers to know whether similar (or different) results were found on:

解析：同一類型的癌癥可能有多種細胞系，因為即使是同一種癌癥，不同的細胞系中往往也會有不同的表現和機制。研究時最好選擇多種細胞系進行實驗。

DMSO is itself an anti-oxidant. It is imperative that appropriate vehicle controls be used. You have to refer to the data indicating whether or not the DMSO vehicle alone had an effect on cell death in any of your experiments.

解析：在設計實驗的時候，一定要考慮所選用的對照是否合理，是否對本實驗的細胞效應有影響。

方法

Although this study was fairly designed and performed, several methods are not clear enough. Moreover, authors should describe the principle of kits used to measure hydroxyl radicals and superoxide in the method.

解析：重要的研究方法敘述要詳細。比如那些用試劑盒的方法或者之前參考文獻的方法，引用之后還需要簡單介紹一下。

It is very important to describe how to control the quality of aqueous extract of the shoot of ××?in each preparation or batch.

解析：對于中藥提取物的實驗，對于提取物成分的鑒定要有描述，比如液相色譜、指紋圖譜之類的。

100 uM is a relatively high dose of the caspase inhibitors used compared to other published reports. You need to indicate why you used that dose. Appropriate vehicle controls are required.?Please convert the r/min to g/min for centrifuge speed.

解析：用藥物或試劑進行細胞處理時，最好給出所用劑量的依據（前人的研究），對照設置要全。試劑和儀器的單位要按照國際慣例，用SCI論文期刊中常用的單位。

For animal model establishment, mice were randomly divided into four groups while the number of mice in each group was not introduced.?Specify type of rats and weights. What about handling and acclimation.

解析：對于動物模型實驗建立后，將模型小鼠隨機分組后，每組小鼠的數量要介紹清楚；動物的品系和體重，大鼠的飼養(yǎng)情況等。這些是動物實驗中最基本的信息，需要在文章初稿中描述清楚。

Was the injection performed under sedation or anesthesia? When were the injections made? Please add the way of administration. For example, add?i.p.?or any other route of administration.

解析：針對動物的操作處理，包括麻醉方式、給藥方式及頻率以及指標檢測等信息都要在手稿中交代清楚。請?zhí)砑觿游锏奈顾幏绞剑菏峭ㄟ^腹腔注射還是其它途徑喂藥？動物實驗中處理動物的詳細信息要交代清楚。模型構建方式最好是經典的方法，手稿中要引用相關的文獻。

Authors described in method part, they treated the mice with 20 mg/kg of bleomycin, have them observed any death?

解析：動物在試劑處理后，有沒有觀察到死亡？即在實驗中由于任何處理因素可能會引起實驗動物死亡或者觀察到實驗動物死亡的都要在方法部分交代清楚，沒有就描述沒有，如果出現死亡，則要描述死亡的實驗動物數以及出現死亡的原因。

Details were needed, such as the country information of chemiluminescence instrument. Beside the names, manufacturers’ states and countries of reagents should be given at the first time, and when the reagents appear again, just list the manufacturers’ names.

解析：對于實驗中的觀察指標的各種儀器，例如各種類型的顯微鏡（普通光學顯微鏡、熒光顯微鏡、激光共聚焦顯微鏡以及電子顯微鏡等）、分光光度劑、灰度掃描儀、血氣分析儀等，其型號、廠家、原產地（城市，州，國家）以及所有試劑都要提供公司信息盡量展示在文章中。試劑或儀器第一次出現時要給出廠商的具體信息，之后再出現的時候只需要給出廠商就可以了。

Statistical Analysis: Did you perform a sample size calculation before the study to determine how many rats you needed in each group? Why use SEM and not SD??Please add the post-hoc test used in one-way ANOVA. I assume the Tukey post test was used.

解析：統(tǒng)計分析部分要說明在實驗之前有無進行樣本量估算來明確每組中大鼠的數目，為什么使用標準誤SEM而不是SD即標準差。大多數情況審稿人不會追究實驗前的樣本量估算過程，我們在文章描述的過程中，只要如實描述實驗中用到的樣本量即可。如果前期我們確實沒有做樣本量估算，在回答審稿人問題的時候，引入相關文獻并說明我們是參照之前報道的動物數目進行實驗的也是可以的。針對標準差與標準誤的問題，標準誤反應的樣本均數間的離散程度，也反映了樣本均數與總體均數的差異，而標準誤描述的數據分布的離散程度。一般在科技論文中需要展示的數據的離散程度即標準差而不是標準誤。此外，在進行整體組間比較后，組與組之間的兩兩比較的多重檢驗也要表述清楚。

結果

If ××?is vehicle control, then it is unclear why ××?group has effect on ×× and whether there are any statistically significant differences between the ××?group and Extract treatment group. Also, in Table 3 and Table 4. Please check the data, result statements, and the Abstract.

解析：結果描述、圖表、摘要里的結果要保持一致，不能出現不符甚至矛盾的情況。

You must not repeat the methods or background in your Results section – citations are not acceptable here – have deleted this reproduction of information.

解析：不要在結果部分重復方法以及研究背景，另外在實驗結果中最好不要援引文獻。結果部分只是展示我們的實驗結果，沒有必要也盡量不要援引文獻。

Any words like “indicating” “suggesting” or “illustrating” were not very suitable to be in Results section because only the results of your research were needed, the analysis section should be put in Discussion.

解析：結果部分不應該出現如“indicating” “suggesting” 以及 “illustrating”等推測性的詞語。結果部分還是主要客觀地描述實驗結果，盡量不要出現實驗方法以及由實驗結果引申出的實驗推論。

The description of Figure 1 is too brief in Result section, please add more statements which could better illustrate the results of this study.

解析：主要結果的描述要詳細，例如組織切片的結果，不能直接說模型組組織形態(tài)被破壞，干預組有明顯的改善效果，要具體描述正常組織形態(tài)、病變組織形態(tài)。添加更多的語句會更好地說明文章的結果。

圖表

There were no statistical significance in Figures.

解析：進行比較的結果展示中要標明統(tǒng)計學結果并在圖注中給出關于統(tǒng)計差異的說明。

Arrows were needed in Figure 1C, Figure 2A, Figure 2B to make it more clear for pointing out important results.

解析：圖1C，2A以及2B中需要用箭頭指出更重要的結果。在一些定性展示的圖片中，如HE染色中圖片中組織特定部位的病變最好用箭頭標注出來。

Scale bars are appropriate for histological images

解析：病理學圖像分析中圖中需要展示比例尺即Bar。也可以在圖注中給出放大倍數（如×200）

There were too many figures in this manuscript. This reviewer suggest to reduce the numbers of figures or combine the figures. Some figures might be provided as supplementary materials.

解析：一篇文章的圖表不宜太多，相關的結果圖片最好能夠整合，如果有很多，一些可以以補充材料的形式提交。

Figures need to be in improved resolution. In particular fig 3, 4 and 6. They can not be published in the present low quality.

解析：圖片清晰度達不到發(fā)表標準，需要重新提供高清圖片。