SouthernBiotech艾美捷山羊抗兔IgM-FITC說明書
SouthernBiotech艾美捷山羊抗兔IgM FITC抗體,用于流式細胞術(shù)、免疫組織化學(xué)和免疫印跡分析。
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SouthernBiotech艾美捷山羊抗兔IgM-FITC基本參數(shù):
Cat#:4020-02
同型:山羊IgG
同型對照:山羊IgG-FITC
特異性:與兔IgM重鏈反應(yīng)
來源:兔IgM高免疫山羊的混合抗血清
交叉吸附:兔IgG;可能與其他物種的免疫球蛋白發(fā)生反應(yīng)
純化方法:瓊脂糖共價連接的混合兔IgM親和層析
結(jié)合:熒光素
緩沖液配方:含有<0.1% die氮化鈉的磷酸鹽緩沖鹽水
克隆性:多克隆
濃度:1.0毫克/毫升
體積:1.0毫升
推薦存儲:2-8攝氏度;避免暴露在陽光下
應(yīng)用:經(jīng)過質(zhì)量測試的相關(guān)格式
應(yīng)用程序包括-ELISA、FLISA
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工作稀釋液:
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ELISA:
AP conjugate 1:2,000?–?1:4,000
HRP conjugate 1:4,000?–?1:8,000
BIOT conjugate 1:5,000?–?1:20,000
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FLISA:
FITC, TRITC, and AF555 conjugates 1:100?–?1:400
PE and AF647 conjugates?≤?1 μg/mL
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SouthernBiotech山羊IgG-TRITC文獻引用:
1. Holdsworth RJ, McKenzie H, Parratt D, Irving AD, Cuschieri A. The role of the spleen in the immune response following naturally acquired exposure to encapsulated bacteria. Int J Exp Pathol. 1990;71:835-43. (ELISA)
2. Taylor-Robinson AW, Phillips RS. B cells are required for the switch from Th1- to Th2-regulated immune responses to Plasmodium chabaudi chabaudi infection. Infect Immun. 1994;62:2490-98. (ELISA)
3. Yamashita T, Freigang S, Eberle C, Pattison J, Gupta S, Napoli C, et al. Maternal immunization programs postnatal immune responses and reduces atherosclerosis in offspring. Circ Res. 2006;99:e51-64. (ELISA, ELISPOT)
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SouthernBiotech致力于高質(zhì)量、高親和純化的二抗的生產(chǎn)、純化和標(biāo)記。還提供各種親和純化的多克隆和單克隆抗體,其中包括:熒光素和酶標(biāo)記物,低內(nèi)毒素/不含疊氮化物(LE/AF)制劑,F(xiàn)(ab)和?F(ab')2片段化抗體...
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艾美捷科技是SouthernBiotech的中國代理商,為科研工作者提供優(yōu)質(zhì)的產(chǎn)品與服務(wù)。