1.百螢D-熒光素鉀鹽工作原理

? ? ? ?D-熒光素是流行的多功能生物發(fā)光底物。螢火蟲熒光素酶/熒光素生物發(fā)光系統(tǒng)存在于螢火蟲（Photinus pyralis）和其他幾種甲蟲中。熒光素酶通過二氧雜環(huán)丁酮中間體氧化ATP進(jìn)行活化熒光素。螢火蟲熒光素酶通過熒光素的ATP依賴性氧化產(chǎn)生光。來自該反應(yīng)的560nm化學(xué)發(fā)光在數(shù)秒內(nèi)達(dá)到峰值，當(dāng)熒光素和ATP過量存在時(shí)，光輸出與熒光素酶活性成比例。螢火蟲熒光素酶長期以來與抗體結(jié)合，并在熒光素作為檢測底物的免疫測定中用作標(biāo)記物。與HRP和堿性磷酸酶相比，熒光素酶對(duì)化學(xué)修飾的耐受性較差。該酶的一個(gè)特別優(yōu)點(diǎn)是除了其高靈敏度之外，在哺乳動(dòng)物組織中存在低內(nèi)源熒光素酶活性。熒光素酶的另一個(gè)重要用途是衛(wèi)生監(jiān)測領(lǐng)域，熒光素酶/熒光素系統(tǒng)可用于檢測污染，因?yàn)榇嬖谟谒猩矬w中的ATP需要發(fā)光，這種ATP生物發(fā)光的主要應(yīng)用是通過測試食品加工廠中的表面來確定質(zhì)量，以確定是否存在設(shè)備或產(chǎn)品的污染。

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2.百螢D-熒光素鉀鹽基本參數(shù)

Ex(nm)? ? ? ?335

Em(nm) ?????530

分子量? ? ? ??318.41

溶? ? 劑 ???????Water

C A S ????????115144-35-9 ???

存儲(chǔ)條件 ???在-15℃以下保存，盡量避免光照

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3.百螢D-熒光素鉀鹽參考文獻(xiàn)

Mutagenesis of solvent-exposed amino acids in Photinus pyralis luciferase improves thermostability and pH-tolerance
Authors:?Law GH, G and elman OA, Tisi LC, Lowe CR, Murray JA.
Journal:?Biochem J?(2006):?305

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Determination of p-glycoprotein ATPase activity using luciferase
Authors:?Matsunaga T, Kose E, Yasuda S, Ise H, Ikeda U, Ohmori S.
Journal:?Biol Pharm Bull?(2006):?560

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Expression of firefly luciferase in Candida albicans and its use in the selection of stable transformants
Authors:?Doyle TC, Nawotka KA, Purchio AF, Akin AR, Francis KP, Contag PR.
Journal:?Microb Pathog?(2006):?69

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PET imaging and optical imaging with D-luciferin [11C]methyl ester and D-luciferin [11C]methyl ether of luciferase gene expression in tumor xenografts of living mice
Authors:?Wang JQ, Pollok KE, Cai S, Stantz KM, Hutchins GD, Zheng QH.
Journal:?Bioorg Med Chem Lett?(2006):?331

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Bioluminescence of monolayers of firefly luciferase immobilized on graphite
Authors:?Palomba S, Berovic N, Palmer RE.
Journal:?Langmuir?(2006):?5451

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Bovine serum albumin displays luciferase-like activity in presence of luciferyl adenylate: insights on the origin of protoluciferase activity and bioluminescence colours
Authors:?Viviani VR, Ohmiya Y.
Journal:?Luminescence?(2006):?262

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Noninvasive bioluminescence imaging of luciferase expressing intracranial U87 xenografts: correlation with magnetic resonance imaging determined tumor volume and longitudinal use in assessing tumor growth and antiangiogenic treatment effect
Authors:?Szentirmai O, Baker CH, Lin N, Szucs S, Takahashi M, Kiryu S, Kung AL, Mulligan RC, Carter BS.
Journal:?Neurosurgery?(2006):?365

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Two different domains of the luciferase gene in the heterotrophic dinoflagellate Noctiluca scintillans occur as two separate genes in photosynthetic species
Authors:?Liu L, Hastings JW.
Journal:?Proc Natl Acad Sci U S A.?(2006)

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Effect of different salts and detergents on luciferin-luciferase luminescence of the enchytraeid Fridericia heliota
Authors:?Rodionova NS, Petushkov VN.
Journal:?J Photochem Photobiol B?(2006):?123

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Evaluation of firefly luciferase bioluminescence mediated photodynamic toxicity in cancer cells
Authors:?Schipper ML, Patel MR, Gambhir SS.
Journal:?Mol Imaging Biol?(2006):?218

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