前言：

如何快速富集特定脂質(zhì)結(jié)合蛋白，為后續(xù)鑒定研究做鋪墊？

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背景：

細(xì)胞內(nèi)大分子之間的相互作用是探索生命奧秘的重要基礎(chǔ)。脂質(zhì)作為生物分子的重要組分,除儲(chǔ)存和運(yùn)輸機(jī)體代謝所需的能量外,脂質(zhì)還作為細(xì)胞膜的組成部分,參與細(xì)胞表面識(shí)別和組織免疫等生物學(xué)功能。蛋白質(zhì)和脂質(zhì)的相互作用異常,往往會(huì)導(dǎo)致相關(guān)疾病的發(fā)生。膜脂主要包括磷脂（Phospholipid）、糖脂（glycolipid）和膽固醇（cholesterol）三種基本類(lèi)型。它們的化學(xué)結(jié)構(gòu)、在生物膜上的含量以及生物學(xué)功能各不相同。

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正文：

Echelon Biosciences/艾美捷 即用型Lipid Beads方案如下：

下面描述的Lipid Beads，是用于蛋白質(zhì)下拉實(shí)驗(yàn)的理想選擇，以鑒定和描述脂質(zhì)結(jié)合蛋白。這些Beads可從細(xì)胞裂解物或體外翻譯的肽的混合物中分離出脂質(zhì)結(jié)合蛋白，可用于SDS-PAGE、WB和質(zhì)譜分析等下游應(yīng)用。

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英文名稱?中文名稱?cat#?規(guī)格

Control Beads?對(duì)照?Beads?P-B000?1ML

Cardiolipin Beads?心磷脂?Beads?P-BCLP?1ML

Ceramide 1-Phosphate (C1P) Beads?神經(jīng)酰胺1-磷酸?(C1P) Beads?P-BC1P?1ML

Ceramide Beads?神經(jīng)酰胺?Beads?P-BCER?1ML

Cholesterol Beads?膽固醇?Beads?P-BCHL?1ML

Flow PIPs: Multiplex Lipid Beads?Flow PIP：多重脂質(zhì)?Beads?P-FB01?100UG

Ins(1,3,4,5)P4 Beads?1,3,4,5-四磷酸肌醇?Beads?Q-B1345?1ML

Ins(1,4,5)P3 Beads?1,4,5-三磷酸肌醇?Beads?Q-B0145?1ML

Lactosylceramide (LacCer) Beads?乳糖神經(jīng)酰胺?(LacCer) Beads?P-B0LC?1ML

LBPA Beads?溶血雙磷脂酸?Beads?P-BLBPA?1ML

LPA Beads?溶血磷脂酸?Beads?L-6101?1ML

Phosphatidic Acid (PA) Beads?磷脂酸?(PA) Beads?P-B0PA?1ML

Phosphatidylcholine (PC) Beads?磷脂酰膽堿?(PC) Beads?P-B0PC?1ML

Phosphatidylethanol (PEth) Beads?磷脂酰乙醇?(PEth) Beads?P-BPETH?1ML

Phosphatidylethanolamine (PE) Beads?磷脂酰乙醇胺?(PE) Beads?P-B0PE?1ML

Phosphatidylserine (PS) Beads?磷脂酰絲氨酸?(PS) Beads?P-B0PS?1ML

PI(3)P PIP Beads?3-磷酸磷脂酰肌醇?Beads?P-B003A?1ML

PI(3,4)P2 PIP Beads?3,4-二磷酸磷脂酰肌醇?Beads?P-B034A?1ML

PI(3,4,5)P3 PIP Beads?3,4,5-三磷酸磷脂酰肌醇?Beads?P-B345A?1ML

PI(3,5)P2 PIP Beads?3,5-二磷酸磷脂酰肌醇?Beads?P-B035A?1ML

PI(4)P PIP Beads?4-磷酸磷脂酰肌醇?Beads?P-B004A?1ML

PI(4,5)P2 PIP Beads?4,5-二磷酸磷脂酰肌醇?Beads?P-B045A?1ML

PI(5)P PIP Beads?5-磷酸磷脂酰肌醇?Beads?P-B005A?1ML

PIP Beads Sample Pack?磷脂酰肌醇?Beads?測(cè)試包?P-B00S?EAPK

PtdIns PIP Beads?磷脂酰肌醇?Beads?P-B001?1ML

S1P Beads?1-磷酸鞘氨醇?Beads?S-6110?1ML

Sphingo Beads?鞘氨?Beads?S-6100?1ML

Sphingo Beads Sample Pack?鞘氨?Beads?測(cè)試包?P-B00SS?EAPK

Sphingomyelin Beads?鞘磷脂?Beads?P-B0SM?1ML

NeoBeads?–?PIP Purification System?NeoBeads - PIP?純化系統(tǒng)?P-B999?20MG

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其中，P-B045A，PI(4,5)P2 PIP Beads?已發(fā)表超過(guò)85篇高影響力文章：

1.Shalhout, Sophia Z., et al. "YAP-dependent proliferation by a small molecule targeting annexin A2."Nature Chemical Biology7 (2021): 767-775.

2.Lee, Jaewoong, et al. "IFITM3 functions as a PIP3 scaffold to amplify PI3K signalling in B cells."Nature7838 (2020): 491-497.

3.Rao, Ling, et al. "p85β?regulates autophagic degradation of AXL to activate oncogenic signaling."Nature communications1 (2020): 1-15.

4.Kim, Sung-Kuk, et al. "Phosphatidylinositol phosphates directly bind to neurofilament light chain (NF-L) for the regulation of NF-L self assembly."Experimental & molecular medicine3 (2011): 153-160.