?A Discussion of Protein Research

What’s the Difference Between Tween 20 and Tween 80??

20和80之間的區(qū)別是什么?

As a non-ionic viscous liquid, tweens are a class of emulsifiers used in pharmaceuticals and food preparation.

?作為一種非離子粘性液體，吐溫是一類(lèi)用于藥品和食品制備的乳化劑。

Tween? 20?and?Tween? 80?are both biocompatible surfactants used in food, biotechnical, and pharmaceutical applications. However, despite their similar uses, there are differences between the two types of tweens.

Tween 20和Tween 80都是生物相容性表面活性劑，用于食品、生物技術(shù)和制藥應(yīng)用。然而，盡管它們的用途相似，但兩種類(lèi)型的補(bǔ)間之間存在差異。

What is Tween??

Otherwise known as polysorbates, tweens are typically used as a surfactant for dispersing hydrophobic particles in aqueous solutions, as well as a non-ionic detergent for selective protein extraction and isolation of nuclei from mammalian cells.

?也稱(chēng)為聚山梨酯，吐溫通常用作表面活性劑，用于分散水溶液中的疏水粒子，以及非離子洗滌劑，用于選擇性蛋白質(zhì)提取和分離哺乳動(dòng)物細(xì)胞的細(xì)胞核。

Tweens are a condensate of sorbitol fatty acid ester and ethylene oxide, and either soluble or dispersible in water but differ widely in organic and oil solubilities.

吐溫是山梨醇脂肪酸酯和環(huán)氧乙烷的凝結(jié)物，可溶于水或分散于水，但在有機(jī)溶解度和油溶解度方面差別很大

What is Tween? 20?

Tween? 20, or Polysorbate 20, is a popular choice for biochemical applications. With a hydrophobic dodecanoic tail, it attached to 20 repeat units of polyethylene glycol and distributed across four different chains.

吐溫20，或聚山梨酯20，是一個(gè)流行的選擇生化應(yīng)用。它有一個(gè)疏水的十二烷尾巴，附著在20個(gè)重復(fù)單元的聚乙二醇上，分布在四個(gè)不同的鏈上。

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As a non-ionic surfactant, Polysorbate 20 has a molecular weight of 1,225 daltons, assuming 20 ethylene oxide units, one sorbitol, and one lauric acid as the primary fatty acid.

?聚山梨醇20作為一種非離子表面活性劑，其分子量為1225道爾頓，假定環(huán)氧乙烷20個(gè)單位，山梨醇1個(gè)單位，月桂酸1個(gè)單位為主要脂肪酸

The ethylene oxide subunits are responsible for the hydrophilic nature of the surfactant, while the hydrocarbon chains provide the hydrophobic environment. Ethylene oxide polymers attach to the backbone ring, which is formed by sorbitol.

環(huán)氧乙烷亞基負(fù)責(zé)表面活性劑的親水性，而碳?xì)滏溙峁┦杷h(huán)境。環(huán)氧乙烷聚合物附著在山梨醇形成的主環(huán)上。

It is also miscible in water (100 mg/ml) and yields a clear, yellow solution. It is practically insoluble in liquid paraffin and fixed oils, and also miscible in alcohol, dioxane, and ethyl acetate.

它在水（100mg / ml）中也會(huì)混溶，得到清澈的黃色溶液。 它實(shí)際上不溶于液體石蠟和固定油，也可以在醇，二惡烷和乙酸乙酯中混溶。

What is Tween? 80?

Tween? 80?is a non-ionic detergent for solubilizing membrane proteins during the isolation of membrane-protein complexes, and is available with ultra-low concentrations of contaminating peroxides, aldehydes, salts, and carbonyl compounds.

?吐溫80是一種非離子洗滌劑，用于在膜蛋白復(fù)合物分離過(guò)程中溶解膜蛋白，可用于污染物過(guò)氧化物、醛、鹽和羰基化合物的超低濃度。

Tween 80 is also a polyethylene sorbitol ester, otherwise known as Polysorbate 80 or polyoxyethylene sorbitan monooleate. It has a molecular weight of 1.31 kDa and works well as a stabilizer and emulsifier, primarily in cosmetics, pharmaceuticals, and food products.

?吐溫80也是一種聚乙烯山梨醇酯，或稱(chēng)為聚山梨醇80或聚氧乙烯山梨醇單油酸酯。它的分子量為1.31 kDa，可以作為穩(wěn)定劑和乳化劑，主要用于化妝品、藥品和食品。

Tween 80 is also miscible in water (0.1 ml/ml) and yields a clear, yellow solution. It is also miscible with alcohol, dioxane, and ethyl acetate, and is practically insoluble in liquid paraffin and fixed oils.

吐溫80也在水（0.1ml / ml）中混溶，得到澄清的黃色溶液。 它也可與乙酸醇，二惡烷和乙酸乙酯混溶，實(shí)際上不溶于液體石蠟和固定油。

What is the Difference Between Tween 20 and Tween 80?

Tween 20 and Tween 80 are both used in food, biotechnical, and pharmaceutical applications. However, they are present in different types of applications.

?Tween 20和Tween 80都用于食品、生物技術(shù)和制藥應(yīng)用。然而，它們存在于不同類(lèi)型的應(yīng)用程序中。

Tween 20 is a common component in the following applications:

吐溫20是以下應(yīng)用中的常見(jiàn)成分：

• The pre-extraction of membranes to remove peripheral proteins （預(yù)萃取膜去除外周蛋白）

• Membrane-based immunoassays （基于膜的免疫測(cè)定）

• Lysing mammalian cells （裂解哺乳動(dòng)物細(xì)胞）

• PCR (polymerase chain reaction（聚合酶鏈反應(yīng)）

• ELISA (enzyme-linked immunosorbent assay)?（酶聯(lián)免疫吸附試驗(yàn)）

• Western blot analysis?印跡分析

Due to its drying property, Tween 80 is typically present in ice cream. It is also widely utilized in biochemical applications, including:

由于其干燥性，吐溫80通常存在于冰淇淋中。 它也廣泛用于生化應(yīng)用，包括：

• Isolating nuclei from mammalian cell lines（從哺乳動(dòng)物細(xì)胞中分離核）

• Growing of tubercle bacilli（生長(zhǎng)結(jié)節(jié)桿菌）

• Selective protein extraction (選擇性蛋白質(zhì)提取)

• Emulsifying and dispersing substances in medicinal and food products (藥品和食品中的乳化和分散物質(zhì))

• As a hydrophilic emulsifier in the preparation of W1/O/W2 emulsion (作為制備W1 / O / W2乳液的親水性乳化劑)