中英文說明書丨艾美捷FD快速高爾基法染色試劑盒
Golgi-Cox impregnation1, 2 has been one of the most effective techniques for studying both the normal and abnormal morphology of neurons as well as glia. Using the Golgi technique, subtle morphological alterations in neuronal dendrites and dendritic spines have been discovered in the brains of animals treated with drugs as well as in the postmortem brains of patients with neurological diseases3, 4. However, the unreliability and the time-consuming process of Golgi staining have been major obstacles to the widespread application of this technique.
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FD Rapid GolgiStain? Kit is designed based on the principle of the methods described by Ramón- Moliner2, Glaser and Van der Loos5. This kit has not only dramatically improved and simplified the Golgi-Cox technique but has also proven to be extremely reliable and sensitive for demonstrating morphological details of neurons and glia, especially dendritic spines. The FD Rapid GolgiStain? Kit has been tested extensively and widely used on the brains from several species of animals as well as on the specimens of postmortem human brains.
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Kit contents:
Store at room temperature
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Solution A ????????????????????????????????????????250 ml
Solution B ????????????????????????????????????????250 ml
Solution C ????????????????????????????????????????250 ml x 2
Solution D ????????????????????????????????????????250 ml
Solution E ????????????????????????????????????????250 ml
Glass Specimen Retriever ?????????????2
Natural hair paintbrush ????????????????2
Dropping bottle ???????????????????????????????1
User Manual ????????????????????????????????????1
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艾美捷FD NeuroTech?FD快速高爾基法染色試劑盒(#PK401)背景:
高爾基體-考克斯浸漬1, 2一直是研究神經(jīng)元以及神經(jīng)膠質(zhì)細胞正常和異常形態(tài)的有效技術(shù)之一。使用高爾基體技術(shù),在接受藥物治療的動物大腦以及神經(jīng)系統(tǒng)疾病患者的死后大腦中發(fā)現(xiàn)了神經(jīng)元樹突和樹突棘的細微形態(tài)變化3, 4.然而,高爾基體染色的不可靠性和耗時的過程一直是該技術(shù)廣泛應(yīng)用的主要障礙。
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FD快速高爾基法染色試劑盒是根據(jù)Ramón-Moliner描述的方法原理設(shè)計的2、格拉澤和范德洛斯5.該試劑盒不僅顯著改進和簡化了高爾基體-考克斯技術(shù),而且還被證明在展示神經(jīng)元和神經(jīng)膠質(zhì)細胞(尤其是樹突棘)的形態(tài)細節(jié)方面非??煽亢挽`敏。FD 快速高爾基體染色?試劑盒已在多種動物的大腦以及死后人類大腦標(biāo)本上進行了廣泛和廣泛的測試。
