重組熒光素酶,解決了天然來源的季節(jié)不穩(wěn)定性和區(qū)域變異性
重組熒光素酶(rLUC)是一種從北美螢火蟲(Photinus pyralis)克隆的基因表達的熒光素素酶,它提供了使用生物發(fā)光試劑檢測ATP或熒光素基進行研究或生產(chǎn)試劑盒所需的可靠性和可靠性。重組來源消除了從天然來源純化的熒光素酶中可能發(fā)現(xiàn)的季節(jié)性和區(qū)域性變異的可能性。
熒光素酶是一種發(fā)光的酶。多種生物利用熒光素酶介導的生物發(fā)光來驚嚇捕食者或吸引獵物或配偶。北美螢火蟲的熒光素酶在其化學底物熒光素的氧化過程中釋放綠光。重組熒光素酶來源于大腸桿菌發(fā)酵。
Recombinant Luciferase is a luciferase ?expressed from a cloned gene from the North American firefly (Photinus ?pyralis) that provides the reliability and dependability needed for ?performing research or producing kits using bioluminescence reagents to ?detect ATP or luciferin substrates. A recombinant source eliminates the ?possibility of seasonal and regional variability that may be found in ?luciferase purified from natural sources.
Luciferases are enzymes that ?emit light. A diverse group of organisms use luciferase-mediated ? bioluminescence to startle predators or to attract prey or mates. The ? luciferase from the North American fire fly releases green light during the oxidation of its chemical substrate, luciferin. Recombinant ? luciferase is expressed inE. coli.