The 17β-Estradiol EIA kit is a colorimetric competitive enzyme immunoassay kit with results in < 3 hours. Absorbance is read at 405 nm. This kit offers specific and save non-radioactive quantification of estradiol with ready-to-use liquid color-coded reagents to reduce error.

Specific with low cross-reactivity to related steroids

Safe and non-radioactive enzymatic assay

Simple and color-coded reagents reduces error

Reliable and reproducible results day-after-day and lot-after-lot

Fully quantitative results that surpass semi-quantitative Western blot analysis

Alternative Name:?Estrogen

Sensitivity:?28.5 pg/ml (range 29.3 - 30,000 pg/ml)

Assay Time:?<3 hours

Applications:?ELISA, Colorimetric detection

Application Notes:?For the quantitative determination of 17β-Estradiol in culture supernatants and saliva from any species. For serum and plasma use ADI-900-174.

Wavelength:?405 nm

Species reactivity:?Species independent

Shipping:?Blue Ice Not Frozen

Long Term Storage:?+4°C

Contents:?GxR IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution

Scientific Background:?Biologically active estrogen, 17β-estradiol, is an important sex hormone, but also makes an important contribution to the high concentrations of estrogens, which are present in malignant breast tissues.

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17β-雌二醇ELISA試劑盒是一種比色競爭酶免疫測定試劑盒，結(jié)果在3小時以內(nèi)。在405 nm處讀取吸光度。該試劑盒使用現(xiàn)成的液體色碼試劑提供雌二醇的特異性和節(jié)省的非放射性定量，以減少誤差。

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17β-雌二醇ELISA試劑盒艾美捷特點：

1.對相關(guān)類固醇具有低交叉反應(yīng)性的特異性

2.安全無放射性酶法測定

3.簡單且顏色編碼的試劑減少了錯誤

4.日復(fù)一日、一批又一批可靠且可重復(fù)的結(jié)果

5.超過半定量蛋白質(zhì)印跡分析的全定量結(jié)果

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17β-雌二醇ELISA試劑盒化學(xué)性質(zhì)：

別名：雌激素

靈敏度：28.5 pg/ml（范圍29.3-30000 pg/ml）

化驗時間：<3小時

應(yīng)用：ELISA，比色檢測

應(yīng)用說明：用于定量測定任何物種的培養(yǎng)上清液和唾液中的17β-雌二醇。血清和血漿使用ADI-900-174。

波長：405 nm

物種反應(yīng)性：物種無關(guān)

運輸：藍(lán)冰未凍結(jié)

長期儲存：+4°C

內(nèi)容：GxR IgG微量滴定板、綴合物、抗體、測定緩沖液3、洗滌緩沖液濃縮液、標(biāo)準(zhǔn)品、pNpp底物、停止溶液

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17β-雌二醇ELISA試劑盒文獻(xiàn)參考：

Organochlorine pesticide dieldrin upregulate proximal promoter (PII) driven CYP19A1 gene expression and increases estrogen production in granulosa cells: D. Sharma, et al.; Reprod. Toxicol. 106, 103 (2021), Abstract;

Encapsulation of mesenchymal stem cells in 3D ovarian cell constructs promotes stable and long-term hormone secretion with improved physiological outcomes in a syngeneic rat model: S. Sittadjody, et al.; Ann. Biomed. Eng. 48, 1058 (2020), Application(s): ELISA using culture supernatants, Abstract; Full Text

A high level of TGF-B1 promotes endometriosis development via cell migration, adhesiveness, colonization, and invasiveness: U.K. Soni, et al.; Biol. Reprod. 100, 917 (2019), Abstract;

CB1 agonism prolongs therapeutic window for hormone replacement in ovariectomized mice: K. Zhang, et al.; J. Clin. Invest. 130, 2333 (2019), Abstract;