組蛋白是真核生物中負(fù)責(zé)染色體纖維核小體結(jié)構(gòu)的基本核蛋白。這種結(jié)構(gòu)由約146 bp的DNA包裹在核小體周圍，核小體是由四種核心組蛋白（H2A、H2B、H3和H4）中的每一種組成的八聚體。染色質(zhì)纖維通過連接組蛋白H1與核小體之間的DNA相互作用進(jìn)一步緊密，形成高階染色質(zhì)結(jié)構(gòu)。該基因無內(nèi)含子，編碼組蛋白H3家族的一個成員。該基因的轉(zhuǎn)錄本缺乏多核苷酸序列；相反，它們包含回文終止元素。

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該基因位于染色體1上的組蛋白簇中。該基因是集群中四個重復(fù)的組蛋白基因之一；該記錄代表端?？截?。抗組蛋白H3是對染色質(zhì)修飾劑、染色質(zhì)研究、組蛋白和修飾組蛋白以及表觀遺傳學(xué)研究感興趣的研究人員的理想選擇。

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免疫印跡和免疫熒光檢測抗組蛋白H3[單甲基Lys4]抗體。這種抗體在免疫細(xì)胞化學(xué)、染色質(zhì)免疫沉淀和斑點(diǎn)雜交中很有用。用戶應(yīng)優(yōu)化反應(yīng)性的具體條件。通過HeLa組蛋白制備裂解液或適當(dāng)?shù)募?xì)胞裂解液或提取物中的Western Blotting，預(yù)期約15.4 kDa的條帶對應(yīng)于組蛋白H3蛋白。Epi Plus? 與Novus Biologicals合作生產(chǎn)抗體。

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Rockland艾美捷組蛋白PTM抗體系列可識別經(jīng)過修飾的組蛋白和相關(guān)的細(xì)胞靶點(diǎn)，并通過多種檢測方法進(jìn)行驗(yàn)證。Epi-Plus抗體對這種修飾是特別靈敏和特異的，并且對相同位點(diǎn)或該蛋白其他氨基酸的其他修飾沒有交叉反應(yīng)。免疫熒光（IF）、免疫沉淀（IP）、染色質(zhì)免疫沉淀（ChIP）、免疫組化（IHC）和蛋白印跡（WB）的分析驗(yàn)證了Epi-Plus抗體的有效性。

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Rockland艾美捷組蛋白PTM抗體（部分推薦）：

600-401-I69?Histone H3 K9me1 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I70?Histone H3 K9me2 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I71?Histone H3 K9me3 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I72?Histone H3 K9ac Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I73?Histone H3 K9ac/K14ac Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I74?Histone H3 pS10 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I75?Histone H3 pS10/pT11 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I76?Histone H3 pT11 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I77?Histone H3 R17me2a Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I78?Histone H3 K18me1 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I79?Histone H3 K18me2 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I80?Histone H3 K18me3 Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I81?Histone H3 K18ac Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant

600-401-I82?Histone H3 K23ac Antibody?50ug?Human, mouse, C. elegans, rat, chicken, Xenopus, Drosophila, plant