1.?百螢 鈣紫綠素500 CytoCalcein 405nm激發(fā)參數(shù)

E x(nm)? ? ? ? ???420

E m(nm)? ? ? ? ? 505

分 子 量? ? ? ? ???517.93

溶? ? ??劑? ? ? ? ??DMSO

存儲條件? ? ? ? ?在零下15度以下保存，避光防潮

保??質(zhì)??期? ? ? ? ?24個(gè)月

激發(fā)波長(nm) ??410

發(fā)射波長(nm) ??500

2.?百螢 鈣紫綠素500 CytoCalcein 405nm激發(fā)簡介及作用

? ? ? ?鈣紫綠素500 CytoCalcein 405nm激發(fā)?是美國AAT Bioquest生產(chǎn)的細(xì)胞活力測定 試劑,CytoCalcein Violet 500設(shè)計(jì)用于以與鈣黃綠素AM相同的方式標(biāo)記活細(xì)胞。它具有在405 nm處的最大激發(fā),與流式細(xì)胞儀的紫色激光線完全匹配,也可以用熒光顯微鏡的激發(fā)源很好地激發(fā)。進(jìn)入活細(xì)胞后,弱熒光的CytoCalcein Violet 500水解為強(qiáng)熒光染料,其最大激發(fā)/發(fā)射波長為405 nm / 500 nm。與典型的FACS熒光團(tuán)的出色光譜分離為多重實(shí)驗(yàn)提供了更多選擇。CytoCalcein Violet 450,CytoCalcein Violet 500,CytoCalcein Blue 550和CytoCalcein Blue 600已開發(fā)用于流式細(xì)胞儀。CytoCalcein 染料顯示出與Calcein,AM類似的生物學(xué)特性。它們針對各種流式細(xì)胞儀的激發(fā)波長進(jìn)行了優(yōu)化,為活細(xì)胞的流式細(xì)胞術(shù)分析提供了其他顏色。CytoCalcein Violet 450和CytoCalcein Violet 500被405 nm的紫色激光充分激發(fā),并分別在450 nm和500 nm發(fā)射熒光。

3.?適用儀器

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流式細(xì)胞儀

激發(fā)：405nm激光

發(fā)射：525/40nm濾波片

推薦孔板：AmCyan通道

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熒光顯微鏡

激發(fā)：DAPI

發(fā)射：DAPI

推薦孔板：黑色透明

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熒光酶標(biāo)儀

激發(fā)：405nm

發(fā)射：500nm

Cutoff：475nm

推薦孔板：黑色孔板

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4.?參考文獻(xiàn)

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors:?Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal:?Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation
Authors:?Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal:?Cellular Signalling (2017)

Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors:?Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal:?MEDICINE (2016): 1--8

Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors:?Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal:?Tissue Engineering Part C: Methods (2016): 322--331

Influence?of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors:?Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez