FD Apop? Kit is designed for the microscopic detection of cells undergoing apoptosis based on the principle of in situ DNA nick-end labeling (TUNEL) technique1. The assay uses terminal deoxynucleotidyl transferase to catalyze the incorporation of biotinylated deoxyuridines onto the free 3′-hydroxyl termini of DNA fragments, which are considered one of the most characteristic features of apoptosis2, 3. The integrated biotins are amplified and visualized with the avidin-biotin-complex (ABC) method4, enabling light microscopic identification.

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The reagents and procedure of FD Apop? Kit have been optimized to achieve a high degree of both specificity and sensitivity for detecting apoptotic cells with minimal background. This kit can be used with frozen and paraffin sections, as well as cultured cells. The procedure of the kit takes approximately 4 hours.

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Kit contents:

Part I (Store at -20°C)

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Digestive Enzyme ???????????????????????????2 ml x 4

Reaction Solution A ????????????????????????2 ml x 2

Reaction Solution B ????????????????????????60 μl

Reaction Solution C ????????????????????????40 μl

Chromogen Solution ???????????????????????20 ml

Part II (Store at 4°C)

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Equilibration Buffer ?????????????????????????20 ml

Detection Reagent ??????????????????????????6 ml

10x Phosphate-Buffered Saline ???250 ml x 2

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艾美捷FD NeuroTechFD Apop TUNEL凋亡檢測(cè)試劑盒（#PK101）設(shè)計(jì)用于基于原位DNA切口標(biāo)記（TUNEL）技術(shù)原理的細(xì)胞凋亡的顯微鏡檢測(cè)。該測(cè)定使用末端脫氧核苷酸轉(zhuǎn)移酶催化生物素化脫氧尿苷摻入DNA片段的游離3′-羥基末端，這被認(rèn)為是細(xì)胞凋亡極為典型的特征之一2, 3.整合的生物素通過親和素-生物素復(fù)合物（ABC）方法進(jìn)行擴(kuò)增和可視化4，可實(shí)現(xiàn)光學(xué)顯微鏡識(shí)別。

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FD NeuroTech?FD Apop TUNEL凋亡檢測(cè)試劑盒的試劑和程序已經(jīng)過優(yōu)化，可實(shí)現(xiàn)高度的特異性和靈敏度，以極小的背景檢測(cè)凋亡細(xì)胞。該試劑盒可用于冷凍和石蠟切片以及培養(yǎng)細(xì)胞。該套件的過程大約需要?4 小時(shí)。

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FD Apop TUNEL凋亡檢測(cè)試劑盒文獻(xiàn)引用：

1.Gavrieli Y, Sherman Y, and Ben-Sasson SA. (1992) Identification of programmed cell death in situ via specific labeling of nuclear DNA fragmentation. J. Cell Biol. 119:493-501.

2.Wyllie AH. (1980) Glucocorticoid-induced thymocyte apoptosis is associated with endogenous endonuclease activation. Nature. 284:555-6.

3.Arends MJ, Morris RG, and Wyllie AH. (1990) Apoptosis: the role of the endonuclease. Amer. J. Pathol. 136:593-608.

4.Hsu SM, Raine L, and Fanger H. (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques: a comparison between ABC and unlabeled antibody (PAP) procedures. J. Histochem. Cytochem. 29:577-80.