1.?產(chǎn)品參數(shù)：

?Ex(nm) ??????????????562 ???????????????????????Em(nm) ???????????????576

分 子 量 ??????????????1015.79? ? ? ? ? ? ? ? ? ?溶?? 劑 ????????????????DMSO

存儲條件 ?????????????在零下15度以下保存，避免光照

?2.?產(chǎn)品概述：

? ? ? ? ?鈣黃綠素紅,AM是美國AAT Bioquest生產(chǎn)的用于檢測活細胞的熒光探針，Calcein AM是用于標記和檢測活細胞細胞功能的流行的熒光探針之一。然而，Calcein AM的單色使得在多色應(yīng)用中不可能使用這種有價值的試劑。例如，由于與GFP相同的顏色，不可能將Calcein AM與GFP-轉(zhuǎn)化細胞組合使用。為了解決Calcein AM的這種顏色限制，我們開發(fā)了Calcein Orange ，Calcein Red 和Calcein Deep Red 。這些新的Calcein AM類似物與Calcein AM結(jié)合使用，可以對活細胞進行多色標記和功能分析。非熒光Calcein Red AM可以輕松進入活細胞并水解產(chǎn)生強熒光Calcein Red 染料?？梢允褂贸Ｒ姷腡RITC / Cy3濾波片組檢測Calcein Red 染料。百螢生物是AAT Bioquest的中國代理商，為您提供優(yōu)質(zhì)的鈣黃綠素紅,AM。

3.?適用儀器：

（1）流式細胞儀

Ex: ?????????????????????532/561nm

Em: ????????????????????585/40nm

?（2）熒光酶標儀

Ex: ?????????????????????540nm

Em: ????????????????????590nm

Cutoff:? ? ? ? ? ? ? ? ?570nm

推薦孔板: ????????????黑色透明底板

讀取模式:? ? ? ? ? ? ?底讀模式

?（3）熒光顯微鏡

Ex: ?????????????????????TRITC濾波片組

Em: ????????????????????TRITC濾波片組

推薦孔板:? ? ? ? ? ? ?黑色透明底板

4.?參考文獻

?A perfused human blood--brain barrier on-a-chip for high-throughput assessment of barrier function and antibody transport

Authors:?Nienke R Wevers, Dhanesh G Kasi, Taylor Gray, Karlijn J Wilschut, Benjamin Smith, Remko van Vught, Fumitaka Shimizu, Yasuteru Sano, Takashi Kanda, Graham Marsh
Journal:?Fluids and Barriers of the CNS (2018): 23

Astragaloside IV protects blood-brain barrier integrity from LPS-induced disruption via activating Nrf2 antioxidant signaling pathway in mice
Authors:?Hongli Li, Ping Wang, Fei Huang, Jinmei Jin, Hui Wu, Beibei Zhang, Zhifei Wang, Hailian Shi, Xiaojun Wu
Journal:?Toxicology and applied pharmacology (2017)

Functional imaging of neuronal activity of auditory cortex by using Cal-520 in anesthetized and awake mice
Authors:?Jingcheng Li, Jianxiong Zhang, Meng Wang, Junxia Pan, Xiaowei Chen, Xiang Liao
Journal:?Biomedical Optics Express (2017): 2599--2610

NINJ2--A novel regulator of endothelial inflammation and activation
Authors:?Jingjing Wang, Jingjing Fa, Pengyun Wang, Xinzhen Jia, Huixin Peng, Jing Chen, Yifan Wang, Chenhui Wang, Qiuyun Chen, Xin Tu
Journal:?Cellular Signalling (2017)

Erythropoietin Stimulates Endothelial Progenitor Cells to Induce Endothelialization in an Aneurysm Neck After Coil Embolization by Modulating Vascular Endothelial Growth Factor
Authors:?Peixi Liu, Yingjie Zhou, Qingzhu An, Yaying Song, Xi Chen, Guo-Yuan Yang, Wei Zhu
Journal:?MEDICINE (2016): 1--8

Flexible Endoscopic Spray Application of Respiratory Epithelial Cells as Platform Technology to Apply Cells in Tubular Organs
Authors:?Anja Lena Thiebes, Manuel Armin Reddemann, Johannes Palmer, Reinhold Kneer, Stefan Jockenhoevel, Christian Gabriel Cornelissen
Journal:?Tissue Engineering Part C: Methods (2016): 322--331

Influence of hypothermia and subsequent rewarming upon leukocyte-endothelial interactions and expression of Junctional-Adhesion-Molecules A and B
Authors:?Nicolai V Bogert, Isabella Werner, Angela Kornberger, Patrick Meybohm, Anton Moritz, Till Keller, Ulrich A Stock, Andres Beiras-Fernandez
Journal:?Scientific reports (2016)

Inhibition of ABC transport proteins by oil sands process affected water
Authors:?Hattan A Alharbi, David MV Saunders, Ahmed Al-Mousa, Jane Alcorn, Alberto S Pereira, Jonathan W Martin, John P Giesy, Steve B Wiseman
Journal:?Aquatic Toxicology (2016): 81--88

Rapid generation of collagen-based microtissues to study cell--matrix interactions
Authors:?Marie-Elena Brett, Alexandra L Crampton, David K Wood
Journal:?Technology (2016): 1--8

Toxicokinetics and toxicodynamics of chlorpyrifos is altered in embryos of Japanese medaka exposed to oil sands process-affected water: evidence for inhibition of P-glycoprotein
Authors:?Hattan A Alharbi, Jane Alcorn, Ahmed Al-Mousa, John P Giesy, Steve B Wiseman
Journal:?Journal of Applied Toxicology (2016)