The 11B11 monoclonal antibody reacts with mouse IL-4 (interleukin-4) a multifunctional 14 kDa cytokine. IL-4 is expressed primarily by activated Th2 cells and NK cells, and at lower levels by mast cells, and basophils. IL-4 signals through the IL-4Rα. Upon receptor binding IL-4 stimulates activated B and T lymphocyte proliferation, and the differentiation of B cells into plasma cells. It also induces B cell class switching to IgE, and up-regulates MHC class II production while decreasing the production of Th1 cells, macrophages, IFNγ, and dendritic cell IL-12. Like other Th2 associated cytokines, IL-4 is involved in the airway inflammation observed in the lungs of patients with allergic asthma. The 11B11 monoclonal antibody has been shown to neutralize the bioactivity of natural or recombinant IL-4.

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Isotype?Rat IgG1, κ

Recommended Isotype Control(s)?InVivoMAb rat IgG1 isotype control, anti-horseradish peroxidase

Recommended Dilution Buffer?InVivoPure pH 7.0 Dilution Buffer

Immunogen?Partially purified native mouse IL-4

Reported Applications?in vivo IL-4 neutralization

in vitro IL-4 neutralization

in vivo IL-4 receptor stimulation (as a complex with IL-4)

Flow cytometry

Western blot

Formulation?PBS, pH 7.0

Contains no stabilizers or preservatives

Endotoxin?<2EU/mg (<0.002EU/μg)

Determined by LAL gel clotting assay

Purity?>95%

Determined by SDS-PAGE

Sterility?0.2 μM filtered

Production?Purified from tissue culture supernatant in an animal free facility

Purification?Protein G

RRID?AB_1107707

Molecular Weight?150 kDa

Storage?The antibody solution should be stored at the stock concentration at 4°C. Do not freeze.

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11B11單克隆抗體與小鼠IL-4（白細胞介素-4）反應，這是一種多功能14kDa細胞因子。IL-4主要由活化的Th2細胞和NK細胞表達，在較低水平上由肥大細胞和嗜堿性粒細胞表達。IL-4通過IL-4Rα發(fā)出信號。受體結(jié)合后，IL-4刺激活化的B和T淋巴細胞增殖，以及B細胞分化為漿細胞。它還誘導B細胞類切換到IgE，并上調(diào)MHC II類的產(chǎn)生，同時減少Th1細胞，巨噬細胞，IFNγ和樹突狀細胞IL-12的產(chǎn)生。與其他Th2相關(guān)細胞因子一樣，IL-4參與過敏性哮喘患者肺部觀察到的氣道炎癥。11B11單克隆抗體已被證明可以中和天然或重組IL-4的生物活性。

艾美捷InVivoMAb 抗小鼠 IL-4#BE0045基本參數(shù)：

同種型?大鼠IgG1， κ

推薦的同種型對照?體內(nèi)MAb大鼠IgG1同種型對照，抗辣根過氧化物酶

推薦的稀釋緩沖液?體內(nèi)純?pH 7.0 稀釋緩沖液

免疫原?部分純化的天然小鼠IL-4

報告的應用程序?體內(nèi)IL-4體外中和IL-4體內(nèi)中

和

IL-4受體刺激（與IL-4復合）

流式細胞術(shù)

蛋白質(zhì)印跡

配方?PBS，pH 7.0

不含穩(wěn)定劑或防腐劑

內(nèi)毒素?<2EU/mg （<0.002EU/μg）

通過LAL凝膠凝血測定法測定

純度?>95%

由?SDS-PAGE 決定

無?菌?0.2 μM 過濾

生產(chǎn)?從無動物設施中的組織培養(yǎng)上清液中純化

純化?蛋白?G

里德?AB_1107707

分子量?150 千達

存儲?抗體溶液應以4°C的儲備濃度儲存。 不要凍結(jié)。

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InVivoMAb抗小鼠IL-4部分文獻引用：

in vitro IL-4 neutralization

Hill, E. V., et al. (2015). "Glycogen synthase kinase-3 controls IL-10 expression in CD4(+) effector T-cell subsets through epigenetic modification of the IL-10 promoter" Eur J Immunol 45(4): 1103-1115.

in vitro IL-4 neutralization

Choi, Y. S., et al. (2015). "LEF-1 and TCF-1 orchestrate TFH differentiation by regulating differentiation circuits upstream of the transcriptional repressor Bcl6" Nat Immunol 16(9): 980-990.

in vitro IL-4 neutralization

Kim, Y. U., et al. (2015). "Regulation of autoimmune germinal center reactions in lupus-prone BXD2 mice by follicular helper T cells" PLoS One 10(3): e0120294.

in vitro IL-4 neutralization

Hou, L., et al. (2015). "The protease cathepsin L regulates Th17 cell differentiation" J Autoimmun. S 0896-8411(15): 30024-X.