ManNAz(四酰化 N-疊氮基乙?;事短前罚?/h1>
2022-11-03 10:28 作者:BIOFOUNT范德生物 | 我要投稿
ManNAz(四?;?N-疊氮基乙?;事短前罚┦且环N疊氮化物標(biāo)記的糖,提供了一種高特異性方法,ManNAz被細(xì)胞轉(zhuǎn)化為疊氮唾液酸衍生物,用于細(xì)胞表面蛋白的N-連接糖基化。主要用于通過體內(nèi)代謝標(biāo)記和化學(xué)選擇性連接研究糖蛋白。
溶解度:Soluble in DMSO
性狀:Solid powder
儲(chǔ)藏條件:Dry, dark and store at 0-4℃ for short term (days to weeks) or -20℃ for long term (Store correctly 2-3years).
案例:
Detection of metabolic labeling with azido-sugars in different cell types using azide-reactive DyLight Dyes
A549, U2OS and HK-2 cells were incubated with 40µM azido-acetylmannosamine in cell culture media for 72 hours and then incubated with 100µM of DyLight 550-Phosphine (yellow). The cells were washed, fixed with 4% paraformaldehyde and counterstained with Hoechst 33342 (blue).
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