Target:?Mouse

Host:?Goat

Format:?Whole IgG

Specificity:?mouse IgG except IgM, Fcγ Fragment Specific

NoCross Reactivity:?Human, Bovine, Rabbit Serum Proteins

Conjugate:?Horseradish Peroxidase

Clonality:?Polyclonal

Purity:Antigen specific purified from antisera by immunoaffinity chromatography using antigens coupled to agarose beads.

Buffer:50% glycerol 0.01M PBS,pH 7.6

Stabilizer:14.5 mg/ml Bovine Serum Albumin (IgG-Free, Protease-Free)
Preservative:?None

Physical State:?Liquid

Storage & Expiration Date:?-20°C. for 24months

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Suggested Working Concentration or Dilution Range:

1:500 -1:5,000 for immunohisto/cytochemistry.

1:5,000-1:100,000 for ELISA and Western blotting with chromogenic substrates
1:10,000 - 1:200,000 for Western blotting with ECL substrates

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Dilution factors are presented in the form of a range because the optimal dilution is a function of many factors, such as antigen density, permeability, etc. The actual dilution used must be determined empirically.

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本產(chǎn)品基于間接ELISA實(shí)驗(yàn)測(cè)試，排除與小鼠IgM外的其他小鼠IgG亞型的非Fab部分反應(yīng)，與血清中非免疫球蛋白的其他組分沒(méi)有交叉反應(yīng)；經(jīng)過(guò)測(cè)試，本產(chǎn)品與人，牛和兔血清蛋白沒(méi)有任何交叉反應(yīng)，不排除與其他物種的血清有交叉反應(yīng)的可能（沒(méi)有測(cè)試其他物種數(shù)據(jù)）。

本二抗產(chǎn)品最顯著的用途是用于間接ELISA方法進(jìn)行非IgM亞型單克隆抗體細(xì)胞株篩選：在單克隆抗體中如果篩選到IgM亞型的抗體非常棘手，本產(chǎn)品將IgM亞型單克隆抗體細(xì)胞株扼殺在搖籃中，基本原理是本二抗不會(huì)針對(duì)IgM亞型產(chǎn)生反應(yīng)，因此篩選的時(shí)候得到的細(xì)胞株全部為非IgM亞型。