【標(biāo)題速讀】【Nmeth】【2022年】【7-12月】
聲明：本專欄主要對生命科學(xué)領(lǐng)域的一些期刊文章標(biāo)題進行翻譯，所有內(nèi)容均由本人手工整理翻譯。由于本人專業(yè)為生物分析相關(guān)，其他領(lǐng)域如果出現(xiàn)翻譯錯誤請諒解。
JeWell microchips facilitate compartmentalized organoid culture and allow single-objective light sheet imaging of up to 96 organoids in 3D and in three colors in one hour.
JeWell微芯片促進了類器官的區(qū)域化培養(yǎng)，并允許在一小時內(nèi)對多達(dá)96個類器官進行3D和三種顏色的單目標(biāo)光片成像。
1.Direct identification of A-to-I editing sites with nanopore native RNA sequencing.
用納米孔原生RNA測序直接識別A-I編輯位點。
2.Emu: species-level microbial community profiling of full-length 16S rRNA Oxford Nanopore sequencing data.
Emu：全長16S rRNA牛津納米孔測序數(shù)據(jù)的物種級微生物群落分析。
3.Cyclic immonium ion of lactyllysine reveals widespread lactylation in the human proteome.
乳酸菌素的環(huán)狀銨離子揭示了人類蛋白質(zhì)組中廣泛的乳酸化。
4.MSNovelist: de novo structure generation from mass spectra.
MSNovelist：從質(zhì)譜中生成新的結(jié)構(gòu)。
5.A general approach for engineering RTKs optically controlled with far-red light.
用遠(yuǎn)紅光進行光學(xué)控制的RTKs工程的一般方法。
6.Automated high-speed 3D imaging of organoid cultures with multi-scale phenotypic quantification.
具有多尺度表型定量的類器官培養(yǎng)物的自動高速三維成像。
7.Multiplexed bioluminescence microscopy via phasor analysis.
通過相位分析的多重生物發(fā)光顯微鏡。
以高時空分辨率監(jiān)測大腦中的血流
Functional ultrasound localization microscopy reveals whole-brain vascular changes during neuronal activation at high resolution, providing quantitative information on changes in flow, speed and vessel diameter in multiple vascular compartments over a wide field of view.
功能性超聲定位顯微鏡以高分辨率揭示神經(jīng)元激活過程中的全腦血管變化，提供關(guān)于寬視野內(nèi)多個血管隔室中流量、速度和血管直徑變化的定量信息。
1.Integrative genome modeling platform reveals essentiality of rare contact events in 3D genome organizations.
綜合基因組建模平臺揭示了三維基因組組織中罕見接觸事件的本質(zhì)。
2.Sprod for de-noising spatially resolved transcriptomics data based on position and image information.
Sprod用于基于位置和圖像信息的空間分辨率轉(zhuǎn)錄組學(xué)數(shù)據(jù)的去噪。
3.PROBER identifies proteins associated with programmable sequence-specific DNA in living cells.
PROBER確定了與活細(xì)胞中可編程序列特異性DNA相關(guān)的蛋白質(zhì)。
4.Real age prediction from the transcriptome with RAPToR.
用RAPToR從轉(zhuǎn)錄組中預(yù)測真實年齡。
5.Directed evolution of adeno-associated virus for efficient gene delivery to microglia.
用于向小膠質(zhì)細(xì)胞有效傳遞基因的腺相關(guān)病毒的定向進化。
6.Photoswitching fingerprint analysis bypasses the 10-nm resolution barrier.
光開關(guān)指紋分析繞過了10納米的分辨率障礙。
7.Self-supervised deep learning encodes high-resolution features of protein subcellular localization.
自我監(jiān)督的深度學(xué)習(xí)編碼了蛋白質(zhì)亞細(xì)胞定位的高分辨率特征。
8.Functional ultrasound localization microscopy reveals brain-wide neurovascular activity on a microscopic scale.
功能性超聲定位顯微鏡顯示了微觀尺度上的全腦神經(jīng)血管活動。
用于快速深層組織免疫標(biāo)記的熱穩(wěn)定抗體
Heat-stabilized antibodies (SPEARS) enable thermally facilitated 3D immunolabeling (THiCK staining) of parvalbumin-expressing cells in a mouse cerebellar hemisphere.
熱穩(wěn)定抗體（SPEARS）可對小鼠小腦半球中表達(dá)小白蛋白的細(xì)胞進行熱促進3D免疫標(biāo)記（THiCK染色）。
1.Cell type-specific inference of differential expression in spatial transcriptomics.
在空間轉(zhuǎn)錄組學(xué)中推斷特定細(xì)胞類型的差異性表達(dá)。
2.scBasset: sequence-based modeling of single-cell ATAC-seq using convolutional neural networks.
scBasset：使用卷積神經(jīng)網(wǎng)絡(luò)對單細(xì)胞ATAC-seq進行基于序列的建模。
3.MIRA: joint regulatory modeling of multimodal expression and chromatin accessibility in single cells.
MIRA：單細(xì)胞中多模式表達(dá)和染色質(zhì)可及性的聯(lián)合調(diào)節(jié)模型。
4.US-align: universal structure alignments of proteins, nucleic acids, and macromolecular complexes.
US-align：蛋白質(zhì)、核酸和大分子復(fù)合物的通用結(jié)構(gòu)比對。
5.Residue-wise local quality estimation for protein models from cryo-EM maps.
從低溫電鏡圖中獲得的蛋白質(zhì)模型的殘基局部質(zhì)量估計。
6.Single-particle cryo-EM structures from iDPC–STEM at near-atomic resolution.
來自iDPC-STEM的單顆粒低溫冷凍電鏡結(jié)構(gòu)，分辨率接近原子級。
7.Antibody stabilization for thermally accelerated deep immunostaining.
用于熱加速深度免疫染色的抗體穩(wěn)定化。
專注于研究非編碼RNA的方法
This month we present a Focus on methods for studying noncoding RNA and future directions for deciphering the regulatory roles of noncoding RNA. The confetti conceptually illustrates the broad diversity of noncoding RNA and the complexity of their biological implications.
本月，我們將重點介紹研究非編碼RNA的方法以及破譯非編碼RNA調(diào)控作用的未來方向。五彩紙屑從概念上說明了非編碼RNA的廣泛多樣性及其生物學(xué)意義的復(fù)雜性。
1.RNA secondary structure packages evaluated and improved by high-throughput experiments.
通過高通量實驗對RNA二級結(jié)構(gòu)包進行評估和改進。
2.ISSAAC-seq enables sensitive and flexible multimodal profiling of chromatin accessibility and gene expression in single cells.
ISSAAC-seq能夠?qū)渭?xì)胞中的染色質(zhì)可及性和基因表達(dá)進行敏感和靈活的多模態(tài)分析。
3.BIONIC: biological network integration using convolutions.
BIONIC：使用卷積的生物網(wǎng)絡(luò)整合。
4.Event-driven acquisition for content-enriched microscopy.
事件驅(qū)動的采集，用于內(nèi)容豐富的顯微鏡。
5.Event-triggered STED imaging.
事件觸發(fā)的STED成像。
6.Cell region fingerprints enable highly precise single-cell tracking and lineage reconstruction.
細(xì)胞區(qū)域指紋實現(xiàn)了高度精確的單細(xì)胞追蹤和血統(tǒng)重建。
7.A fluorescent sensor for real-time measurement of extracellular oxytocin dynamics in the brain.
用于實時測量大腦中細(xì)胞外催產(chǎn)素動態(tài)的熒光傳感器。
8.A bead-based method for high-throughput mapping of the sequence- and force-dependence of T cell activation.
一種基于珠子的方法，用于高通量繪制T細(xì)胞激活的序列和力依賴性。
9.De novo construction of T cell compartment in humanized mice engrafted with iPSC-derived thymus organoids.
用iPSC衍生的胸腺器官移植的人源化小鼠中T細(xì)胞區(qū)的重新構(gòu)建。
影像之美
The winning image of the Nikon Small World 2022 Photomicrography Competition, an embryonic foot of a Madagascar giant day gecko (Phelsuma grandis). The image was captured using whole-mount fluorescence staining, tissue clearing, high-resolution confocal microscopy and image stitching.
尼康小世界2022年顯微攝影比賽的獲獎圖片，一只馬達(dá)加斯加巨日壁虎（Phelsuma grandis）的胚胎腳。 該圖像是使用整體熒光染色、組織透明化、高分辨率共聚焦顯微鏡和圖像拼接捕獲的。
1.Improved AlphaFold modeling with implicit experimental information.
用隱含的實驗信息改進AlphaFold建模。
2.Profiling RNA at chromatin targets in situ by antibody-targeted tagmentation.
通過抗體靶向標(biāo)記在原位對染色質(zhì)目標(biāo)的RNA進行分析。
3.Light-Seq: light-directed in situ barcoding of biomolecules in fixed cells and tissues for spatially indexed sequencing.
Light-Seq：對固定細(xì)胞和組織中的生物大分子進行光定向原位條碼化，用于空間索引測序。
4.ClampFISH 2.0 enables rapid, scalable amplified RNA detection in situ.
ClampFISH 2.0實現(xiàn)了快速、可擴展的原位擴增RNA檢測。
5.Annotation of spatially resolved single-cell data with STELLAR.
用STELLAR對空間分辨率的單細(xì)胞數(shù)據(jù)進行注釋。
6.Resolution doubling in light-sheet microscopy via oblique plane structured illumination.
通過斜面結(jié)構(gòu)照明實現(xiàn)光片顯微鏡的分辨率翻倍。
7.Incorporating the image formation process into deep learning improves network performance.
將圖像形成過程納入深度學(xué)習(xí)，提高了網(wǎng)絡(luò)性能。
8.Omnipose: a high-precision morphology-independent solution for bacterial cell segmentation.
Omnipose：一種用于細(xì)菌細(xì)胞分割的高精度形態(tài)無關(guān)的解決方案。
9.Geometric engineering of organoid culture for enhanced organogenesis in a dish.
培養(yǎng)類器官的幾何工程，以增強皿中的器官生成。
10.Sensitive genetically encoded sensors for population and subcellular imaging of cAMP in vivo.
敏感的基因編碼傳感器用于體內(nèi)cAMP的群體和亞細(xì)胞成像。
11.neuromaps: structural and functional interpretation of brain maps.
neuromaps：腦圖的結(jié)構(gòu)和功能解釋。
12.Netie: inferring the evolution of neoantigen–T cell interactions in tumors.
Netie：推斷腫瘤中新抗原-T細(xì)胞相互作用的演變。
13.CODA: quantitative 3D reconstruction of large tissues at cellular resolution.
CODA：以細(xì)胞分辨率對大型組織進行定量三維重建。
14.Estimation of skeletal kinematics in freely moving rodents.
自由運動的嚙齒動物的骨骼運動學(xué)的估計。
通過測序共同分析細(xì)胞外蛋白復(fù)合物和 mRNA
Proximity-sequencing (Prox-seq) uses DNA-barcoded antibody probes to detect proteins and their pairwise complexes on the surface of single cells.
鄰近測序（Prox-seq）使用DNA條形碼抗體探針檢測單細(xì)胞表面的蛋白質(zhì)及其成對復(fù)合物。
1.Quantification of extracellular proteins, protein complexes and mRNAs in single cells by proximity sequencing.
通過近距離測序?qū)渭?xì)胞中的細(xì)胞外蛋白、蛋白復(fù)合物和mRNA進行量化。
2.Detection of m6A from direct RNA sequencing using a multiple instance learning framework.
使用多實例學(xué)習(xí)框架從直接RNA測序中檢測m6A.
3.A framework for detecting noncoding rare-variant associations of large-scale whole-genome sequencing studies.
檢測大規(guī)模全基因組測序研究的非編碼罕見變異關(guān)聯(lián)的框架。
4.Chemically stable fluorescent proteins for advanced microscopy.
用于高級顯微鏡的化學(xué)穩(wěn)定熒光蛋白.
5.Image-seq: spatially resolved single-cell sequencing guided by in situ and in vivo imaging.
Image-seq：由原位和體內(nèi)成像指導(dǎo)的空間分辨率單細(xì)胞測序。
6.Cellpose 2.0: how to train your own model.
Cellpose 2.0：如何訓(xùn)練自己的模型。
7.Capturing the start point of the virus–cell interaction with high-speed 3D single-virus tracking.
用高速三維單病毒追蹤捕捉病毒-細(xì)胞相互作用的起始點。
8.Unsupervised discovery of tissue architecture in multiplexed imaging.
多重成像中組織結(jié)構(gòu)的無監(jiān)督發(fā)現(xiàn)。
