免疫組化筆SuperHT PAP Pen，保存珍貴的抗體和試劑
Cat.# Product Name Unit Size
Catalog Number:
Mini Super HT Pap Pen: 22005
Super HT Pap Pen: 22006
Unit Size: 1 pen
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Size:
Mini Super HT Pap Pen: 2.5 mm tip, ~400 applications Super HT Pap Pen: 4 mm tip, ~800 applications
Number of applications varies depending on barrier size
Storage and handling: Store at room temperature. The pens contain organic solvents. Avoid skin and eye contact and keep away from open flame. Cap tightly after each use.
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Product Description
Super HT Pap Pens are used to create hydrophobic barriers around tissue sections on glass slides to hold staining solutions in place on the slide. This allows the conservation of antibody staining solution or staining of two sections on the same slide with different solutions. The Mini Super HT Pap Pen has a fine point that is useful for separating multiple sections on the same slide. Super HT Pap Pen barriers
are insoluble in aqueous buffers, detergents, alcohol and acetone, but can be removed with xylene. Barriers are stable at temperatures up to 120oC.
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Directions
Before first use, fill the tip with barrier fluid. Press the tip down on a clean glass microscope slide to open the tip valve (Figure 1). Keep pressing down until you see the tip fill completely with green liquid. Stop pressing as soon as the tip is completely filled.
Barriers should be created after deparaffinization and rehydration for paraffin sections. For frozen sections, create barriers when slides are dry, before the first buffer incubation step. To create a barrier, dry the area around the section with a cotton swab if necessary. Draw an unbroken circle of Pap Pen fluid around the section (Figure 2). Do not press the valve down while drawing the barrier. Take care to prevent the Pap Pen fluid from touching the tissue section. Let the barrier dry 15-30 seconds before immersing slides or adding buffer. Dry the area immediately outside the barrier if necessary and add just enough buffer to fill the barrier without overflowing. A square of Parafilm can be placed on top of the section to spread the buffer evenly and prevent evaporation. For overnight incubations, slides should be placed in a humidified chamber to prevent evaporation.
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艾美捷免疫組化筆?SuperHT PAP Pen#BTM-22006使用方法：
圖1：第一次使用前先填充筆尖。A、 筆尖包裝干燥。B、 新聞提示向下靠在顯微鏡載玻片上，直到尖端完全充滿綠色液體（C）。
圖2：制造障礙。A、 在剖面周圍畫一個(gè)不間斷的圓圈用巴氏筆。B、 將緩沖液移液到屏障中。添加足夠的緩沖區(qū)填充屏障而不溢出（C）。
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免疫組化筆?SuperHT PAP Pen特征：
在載玻片上的組織樣本周圍繪制防水屏障
保存珍貴的抗體和試劑
屏障不溶于水性緩沖液、洗滌劑、酒精和丙酮
阻隔層在高達(dá)?120°C?的溫度下保持穩(wěn)定
筆含有疏水性有機(jī)溶劑
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免疫組化筆?SuperHT PAP Pen規(guī)格：
迷你超高溫巴氏筆：2.5毫米筆尖，約400次使用
Super HT Pap筆：4毫米筆尖，約800次使用
應(yīng)用程序的數(shù)量因屏障大小而異
