GFAP is a marker for mature glial cells. This antibody is polyclonal and was raised in rabbit to the?peptide sequence – NAGFKETRASERAE (human, rat and mouse) – that was coupled to thyroglobulin by glutaraldehyde before being injected into the rabbit. The antibody has been used for immunocytochemistry in rat and human brain tissue. The vial contains about 200ul at a dilution of 1/100. The antibody can be further diluted to at least 1/30,000 in BSA diluent (50mM KPBS, 0.4% Triton, 1% BSA, 1% NGS). The antibody should be stored at -20C. Also included is filter paper absorbed with the specific peptide for blocking. 500ul of the diluted antibody can be added to the tube to achieve a 10 uM block. Incubate for at least 1hr at room temperature (RT) before applying to tissue. The antibody blocks specifically with this peptide and does not block with other peptides absorbed on filter paper.

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GFAP是一種3型中間絲作為中樞神經(jīng)系統(tǒng)發(fā)育期間的細(xì)胞特異性標(biāo)記以區(qū)分星形膠質(zhì)細(xì)胞與其他膠質(zhì)細(xì)胞。GFAP抗體已經(jīng)被廣泛證實僅識別星形膠質(zhì)細(xì)胞(astrocyte,也稱astroglia),而不識別神經(jīng)細(xì)胞、成纖維細(xì)胞、少突膠質(zhì)細(xì)胞(oligodendrocyte)以及腫瘤細(xì)胞等其它類型的細(xì)胞。

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艾美捷GFAP抗體是多克隆的，在兔體內(nèi)培養(yǎng)至肽序列NAGFKETRASERAE（人、大鼠和小鼠），通過戊二醛，然后注射到兔子體內(nèi)。該抗體已用于大鼠和人腦組織的免疫細(xì)胞化學(xué)。

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小瓶中含有約200ul，稀釋度為抗體可在BSA稀釋劑（50mM KPBS、0.4%Triton、1%BSA、1%NGS）中進一步稀釋至至少1/30000。抗體應(yīng)儲存在-20℃。還包括濾紙用于阻斷的特異性肽?？蓪?00ul稀釋的抗體加入試管中uM塊。在室溫（RT）下孵育至少1小時，然后施用于組織?？贵w與該肽特異性阻斷，而不與濾紙上吸收的其他肽阻斷。

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GFAP抗體（FC50001）基本參數(shù)：

中文名稱：GFAP抗體

英文名字：GFAP Antibody -W

cat#：FC50001

規(guī)格：200ul

應(yīng)用類型：IHC

免疫原：這種抗體是多克隆抗體，在兔體內(nèi)產(chǎn)生的肽序列是NAGFKETRASERAE（人、大鼠和小鼠），在注射到兔體內(nèi)之前，用戊二醛將其與甲狀腺球蛋白偶聯(lián)。

來源宿主：兔

反應(yīng)性：人，大鼠

保存建議：小瓶中含有約200微升1/100稀釋液。 抗體可在BSA稀釋液（50 mM KPBS、0.4%Triton、1%BSA、1%NGS）中進一步稀釋至至少1/20，000。 抗體應(yīng)在-20 ℃下儲存。 還包括吸附有用于封閉的特異性肽的濾紙。 可向管中加入500 μ l稀釋的抗體以獲得10 μ M阻斷。 應(yīng)用于組織前，在室溫（RT）下孵育至少1小時。 抗體特異性地與該肽阻斷，而不與吸附在濾紙上的其他肽阻斷。

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GFAP抗體（FC50001）基本操作：

Fresh frozen 10-30 micron sections are fixed in 4% paraformaldehyde (FA) for 1 hour at RT, washed in phosphate buffered saline (PBS) and incubated with 0.1 to 0.3% H2O2 in PBS for 10 to 30 minutes. The tissue is again washed in PBS, then treated with an Avidin/Biotin blocking kit (Vector-cat #SP-2001), washed and incubated with BSA diluent for 1 hr at RT. TheGFAP antibody (1/30,000) is applied to the tissue overnight at RT. After washing in PBS the sections are incubated in Biotinylated GAR at 1/1000 (Vector labs-cat#BA1000) for 1hr at RT, washed and then incubated in Avidin/Biotin at 1/1000 (Vector labs-cat#PK6100) for 1hr at RT. After washing the signal is visualized with Diaminobenzidine (0.04%) in 0.1M Sodium Acetate with 0.06% H2O2 for six minutes (Nickel Chloride at 2.5% may also be added). Sections are then water washed (counterstained if desired), dehydrated and cover slipped.

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GFAP抗體（FC50001）也適用于BRDU抗體可視化方案。可以使用4%FA灌注組織代替新鮮冷凍切片。

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