艾美捷PolyMag CRISPR試劑盒#PNC40200高轉(zhuǎn)染效率無毒性，靈敏性高、特異性強(qiáng)，用途范圍廣，具體特色如下：

? 即使在原代細(xì)胞和難以轉(zhuǎn)染的細(xì)胞上也能高效遞送 CRISPR/Cas9 系統(tǒng)? 非常適合共轉(zhuǎn)染 pDNA/pDNA、pDNA/gRNA、pDNA/mRNA

? 針對(duì)含有 Cas9 的大質(zhì)粒 DNA 進(jìn)行了優(yōu)化，并引導(dǎo) RNA

? 低細(xì)胞毒性 - 能夠以更少的細(xì)胞開始實(shí)驗(yàn)

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PolyMag CRISPR kit is the only magnet assisted transfection reagent optimized to deliver high level of plasmid DNA and/or mRNA expressing Cas9 and guide RNA (gRNA). This reagent is based on our proprietary Magnetofection? technology. The nanoparticles are composed of iron oxide magnetic core coated with cationic molecules. For your gene editing experiments, this reagent provides high transfection efficiency without toxicity.

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Benefits:

? ???High efficiency delivery of CRISPR/Cas9 system even on primary and hard to transfect cells

? ???Ideal for co-transfection of pDNA/pDNA, pDNA/gRNA, pDNA/mRNA

? ???Optimized for large plasmid DNA containing Cas9, and guide RNA

? ???Low cell toxicity - enable to start your experiment with less cell

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用于CRISPR Cas9實(shí)驗(yàn)的其他產(chǎn)品：

ViroMag CRISPR提高CRISPR/Cas9病毒的轉(zhuǎn)導(dǎo)效率

RmesFect CRISPR 用于 mRNA 轉(zhuǎn)染

ProDeliverIN CRISPR用于 Cas9 蛋白遞送

儲(chǔ)存：?+4°C

運(yùn)輸條件：室溫

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Figure1 : PolyMag CRISPR co-transfection efficiency for CRISPR-Cas9 experiments. NIH-3t3, HeLa and MDCK cells were plated in 24-well plates in complete medium at the optimum density of 50-70% confluence at the time of transfection. Cells were transfected with a GFP-Cas9 plasmid and a RFP pDNA and then incubated overnight at 37oC. GFP and RFP expression was monitored 24h after transfection by fluorescence microscopy.