Cat. No. Format Size

7200-01 Purified (UNLB) 5.0 mg

7200-02 Fluorescein (FITC) 1.0 mg

7200-03 Rhodamine (TRITC) 1.0 mg

7200-04 Alkaline Phosphatase (AP) 1.0 mL

7200-05 Horseradish Peroxidase (HRP) 1.0 mL

7200-06?β-galactosidase (BGAL) 1.0 mL

7200-07 Texas Red??(TXRD) 1.0 mg

7200-09 R-phycoerythrin (PE) 0.5 mg

7200-11 Allophycocyanin (APC) 0.1 mg

7200-12 Cyanine 3 (CY3) 0.1 mg

7200-13 Spectral Red? (SPRD) 0.1 mg

7200-15 Cyanine 5 (CY5) 1.0 mg

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艾美捷?SouthernBiotech??HRP標(biāo)記Avidin親和素描述：

特異性：生物素

來源：中和（pI=6.3±0.3），從蛋清中純化去糖基化抗生物素

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艾美捷?SouthernBiotech ?HRP標(biāo)記Avidin親和素應(yīng)用：

經(jīng)過質(zhì)量測(cè)試的應(yīng)用包括——

ELISA

FLISA

FC

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其他參考應(yīng)用包括——

ELISPOT

IHC-FS

IHC-PS

ICC

WB

Microarray

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艾美捷?SouthernBiotech??HRP標(biāo)記Avidin親和素工作稀釋液：

流式細(xì)胞術(shù)：

FITC和CY3共軛物≤?1μg/106個(gè)細(xì)胞

PE、APC、SPRD和CY5共軛物≤?0.2μg/106個(gè)細(xì)胞

對(duì)于流式細(xì)胞術(shù)，建議使用這些試劑的最終體積為100μL

FLISA：

FITC、TRITC、TXRD和CY3共軛物1:100–1:400

PE、APC和CY5共軛物≤?1微克/毫升

ELISA：

AP共軛1:500–1:2000

HRP共軛1:4000–1:8000

BGAL共軛1:500

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SouthernBiotech HRP標(biāo)記Avidin親和素部分參考文獻(xiàn)：

1. Brown MP, Topham DJ, Sangster MY, Zhao J, Flynn KJ, Surman SL, et al. Thymic lymphoproliferative disease after successful correction of CD40 ligand deficiency by gene transfer in

mice. Nat Med. 1998;4:1253-60. (ELISA)

2. Reinagel ML, Taylor RP. Transfer of immune complexes from erythrocyte CR1 to mouse macrophages. J Immunol. 2000;164:1977-85. (ELISA, FC)

3. Hodge LM, Marinaro M, Jones HP, McGhee JR, Kiyono H, Simecka JW. Immunoglobulin A (IgA) responses and IgE-associated inflammation along the respiratory tract after mucosal

but not systemic immunization. Infect Immun. 2001;69:2328-38. (ELISA, ELISPOT)

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