中英文說(shuō)明書丨艾美捷 RmesFect CRISPR轉(zhuǎn)染試劑
RmesFect? CRISPR transfection Reagent based on the TEE-technology is specifically designed for mRNA/gRNA transfection with high efficiency and low toxicity. RmesFect? is efficient in a large variety of cells.
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Benefits:
Highly efficient: achieve RNAs delivery in all cells
Ready-to-use: no need for additional buffer
Low nucleic acid amount : minimized toxicity
Protects RNAs against degradation
Compatible with any culture medium
Serum compatible : Medium changed not required
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mRNA transfection provides several advantages over plasmid DNA delivery :
No need for nuclear uptake. mRNA translation into proteins occurs in the cytoplasm.
Faster protein expression than DNA transfection
No genomic integration
Protein expression in a total promoter-independent manner
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Additional products for CRISPR Cas9 experiments:
PolyMag CRISPR for Genome editing using expression plasmids
ProDeliverIN CRISPR for Cas9 protein delivery
ViroMag CRISPR to enhance transduction efficiency of CRISPR/Cas9 viruses
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基于TEE技術(shù)的艾美捷 RmesFect? CRISPR轉(zhuǎn)染試劑專為mRNA/gRNA轉(zhuǎn)染而設(shè)計(jì)，具有高效率和低毒性的特點(diǎn)。RmesFect?在多種細(xì)胞中都是有效的。
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OZ Biosciences?RmesFect CRISPR轉(zhuǎn)染試劑特點(diǎn)：
高效：在所有細(xì)胞中實(shí)現(xiàn)RNA遞送
即用型：無(wú)需額外的緩沖液
低核酸量：毒性最小化
保護(hù)?RNA 免受降解
與任何培養(yǎng)基兼容
血清兼容：無(wú)需更換培養(yǎng)基
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圖?1：根據(jù)標(biāo)準(zhǔn)方案，使用?RmesFect CRISPR?轉(zhuǎn)染編碼?GFP?和?RFP（各?24.0μg）的?mRNA?轉(zhuǎn)染?DC25?細(xì)胞。轉(zhuǎn)染后24h在顯微鏡熒光下分析圖像。
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與質(zhì)粒DNA遞送相比，mRNA轉(zhuǎn)染具有以下優(yōu)勢(shì)：
無(wú)需核吸收。mRNA翻譯成蛋白質(zhì)發(fā)生在細(xì)胞質(zhì)中。
蛋白表達(dá)速度快于DNA轉(zhuǎn)染
無(wú)基因組整合
以完全啟動(dòng)子非依賴的方式表達(dá)蛋白質(zhì)
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用于CRISPR Cas9實(shí)驗(yàn)的其他產(chǎn)品：
PolyMag CRISPR用于使用表達(dá)質(zhì)粒進(jìn)行基因組編輯
ProDeliverIN CRISPR用于 Cas9 蛋白遞送
ViroMag CRISPR提高CRISPR/Cas9病毒的轉(zhuǎn)導(dǎo)效率
