RNase-Free DNase I Kit

Norgen's RNA purification kits isolate total RNA with minimal amounts of genomic DNA contamination. However, for some sensitive downstream applications, it may be desirable to remove all traces of residual DNA. Norgen's RNase-free DNAse I Kit, with Enzyme Incubation Buffer, can be used for optional on-column DNase digestion with any of Norgen's RNA purification kits. Alternatively, after isolating total RNA using one of Norgen's RNA purification kits, the RNA elution can be treated with this DNase I. The RNA can then be purified from the DNase using Norgen's RNA Clean-Up and Concentration Kit (Cat# 23600), and the RNA can then be used in downstream applications.

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Each RNase-Free DNase I Kit is supplied complete with sufficient enzyme and enzyme incubation buffer for 50 or 200 reactions.

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For DNase digestion during RNA purification either on-column or in-solution

Convenient optimized on-column DNase treatment using Norgen's RNA Purification Kits

Also includes protocol for digestion in-solution followed by RNA Clean-Up

Guaranteed RNase-Free

Includes Enzyme Incubation Buffer

Cat. 25710 contains one vial of 1,600 units and Cat. 25720 contains 4 vials (1,600 units/vial)

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Storage Conditions

The DNase I provided is in lyophilized form. It is stable for at least 3 months if stored at room temperature. However, it is recommended to store the DNase I vial at 2 – 8oC (or below) upon receipt to maintain stability beyond 3 months. Buffer DR and Enzyme Incubation Buffer can be stored at room temperature. After reconstitution with Buffer DR (see product manual), the DNase I should be stored at -20oC. All reagents should remain stable for at least 1 year in their unopened containers at the appropriate storage temperature.

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艾美捷Norgen無RNase的DNase I試劑盒#NGB-25710：可分離出總RNA，而基因組DNA污染量極少。然而，對于一些敏感的下游應(yīng)用，可能需要去除所有殘留DNA的痕跡。Norgen的無RNaseDNA酶I試劑盒帶有酶孵育緩沖液，可用于使用Norgen的任何RNA純化試劑盒進(jìn)行可選的柱上DNase酶消化?；蛘?，在使用Norgen的RNA純化試劑盒分離總RNA后，可以用該DNase I處理RNA洗脫。然后可以使用Norgen的RNA凈化和濃縮試劑盒（Cat# 23600）從DNA酶中純化RNA，然后RNA可用于下游應(yīng)用。

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無RNase的DNase I試劑盒特點：

使用Norgen的RNA純化試劑盒方便地優(yōu)化了柱上DNase處理；還包括在溶液中消化的方案，然后是RNA清潔保證無RNase；包括酶培養(yǎng)緩沖液；#25710含有一小瓶1600單位和#25720包含4個小瓶（1600個單位/小瓶）。

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儲存條件：

提供的脫氧核糖核酸酶是凍干形式。如果在室溫下儲存，它可以穩(wěn)定至少?3 個月。但是，建議在收到時將 DNase I 小瓶儲存在 2 – 8oC（或更低）的溫度下，以保持穩(wěn)定性超過 3 個月。緩沖液DR和酶孵育緩沖液可在室溫下儲存。用緩沖液DR復(fù)溶后DNase I應(yīng)儲存在-20oC。所有試劑應(yīng)在適當(dāng)?shù)膬Υ鏈囟认略谄湮撮_封的容器中保持穩(wěn)定至少 1 年。