AdenoMag Transfection Reagent is a magnetic nanoparticles formulation specifically dedicated to enhance Adenovirus and Adeno Associated Virus (AAV) infection and transduction capacities. Based on the Magnetofection? technology, it allows concentrating the complete applied dose of viral particles onto cells within minutes, inducing a significant improvement of virus infectivity with extremely low vector doses. Due to its specific properties, AdenoMag is applicable to all adenoviral and AAV vectors and is ideal to infect non permissive cells. This reagent demonstrates an exceptionally high efficiency to promote, control and assist Adenoviral and AAV transductions in a way that no molecular biology processes or biochemical modifications are required.

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Increase transduction efficiency

Accelerate the transduction process

Efficient for hard-to-infect and non-permissive cells

Concentrate the viral dose onto the cells

Synchronise cells adsorption/infection without modifications of the viruses

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AdenoMag transduction reagent:

Sizes:

100 μL: 500-100 transductions based on MOI of 1 for 104 cells per 96 wells

200 μL: 1000-2000 transductions based on MOI of 1 for 104 cells per 96 wells

1000 μL: 5000-10000 transductions based on MOI of 1 for 104 cells per 96 wells

#KC30900: AdenoMag Starting Kit with Super Magnetic Plate + 200 μL of AdenoMag

Storage: +4°C

Shipping Conditions: Room temperature

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艾美捷OZ Biosciences?AdenoMag轉(zhuǎn)染試劑是一種磁性納米顆粒配方，專門用于增強腺病毒和腺相關病毒（AAV）感染和轉(zhuǎn)導能力。基于 磁感應?技術，它允許在幾分鐘內(nèi)將病毒顆粒的全部施用劑量集中在細胞上，從而以極低的載體劑量顯著提高病毒感染性。由于其特殊特性，AdenoMag適用于所有腺病毒和AAV載體，是感染非許可細胞的理想選擇。該試劑在促進、控制和輔助腺病毒和AAV轉(zhuǎn)導方面表現(xiàn)出極高的效率，無需分子生物學過程或生化修飾。

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OZ Biosciences?AdenoMag轉(zhuǎn)染試劑#AM70100特點：

提高轉(zhuǎn)導效率

加速轉(zhuǎn)導過程

對難以感染和非允許的細胞有效

將病毒劑量集中在細胞上

同步細胞吸附/感染，無需修飾病毒

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OZ Biosciences?AdenoMag轉(zhuǎn)染試劑：

規(guī)格：

100 μL：500-100 次轉(zhuǎn)導，基于 MOI 為 1，每 10 孔4 個 96 個細胞

200 μL：1000-2000 次轉(zhuǎn)導，基于 MOI 為 1，每 10 孔4 個 96 個細胞

1000 μL：5000-10000 次轉(zhuǎn)導，基于 MOI 為 1，每 10 孔4 個 96 個細胞

#KC30900：帶超級磁性板 + 200 μL AdenoMag 的 AdenoMag 啟動套件

儲存：?+4°C

運輸條件：室溫

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OZ Biosciences?AdenoMag轉(zhuǎn)染試劑的應用：

是使用所有腺病毒和AAV載體增強細胞轉(zhuǎn)導的理想選擇

適用于所有哺乳動物細胞：細胞系、原代細胞、難轉(zhuǎn)染和非允許細胞

推薦用于：腺病毒和腺相關病毒體外轉(zhuǎn)導?（參見細胞轉(zhuǎn)染數(shù)據(jù)庫和引文數(shù)據(jù)庫）。

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OZ Biosciences?AdenoMag轉(zhuǎn)染試劑的結(jié)果圖：

圖1：腺瘤增強轉(zhuǎn)基因表達：在感染Ad-LacZ、Ad-LacZ與聚苯乙烯（Ad-LacZ + Pb）或Ad-LacZ與293 μL AdenoMag （Ad-LacZ + AM）感染3小時后，在HEK-3，NIH-6T7，C24和COS2細胞系中測定β-半乳糖苷酶表達。

圖2：AdenoMag增強了感染細胞的數(shù)量：感染Ad-GFP（MOI為5）和AdenoMag的0（d），0.5（e）或1 μL（f）的HUVEC的代表性圖像。感染48小時后在白場（a，b和c）和熒光（d，e和f）下拍攝照片。