【文獻速遞】【NBE】【2022年】【2-3月】
聲明：本專欄主要對生命科學領域的一些期刊文章標題進行翻譯，所有內(nèi)容均由本人手工整理翻譯。由于本人專業(yè)為生物分析相關，其他領域如果出現(xiàn)翻譯錯誤請諒解。
Prime editing of pathogenic mutations in mouse eyes and livers
小鼠眼睛和肝臟致病突變的初始編輯
This issue highlights microfluidic sorting for the recovery of potent tumour-infiltrating lymphocytes, the generation of potent CAR T cells in one day, systemic anticancer immunity via intratumourally injected alum-tethered cytokines, synergistic checkpoint blockade and radiotherapy–radiodynamic therapy via nanoscale metal–organic frameworks, in vivo genome editing via disruption of Cas9 activity in the liver, a brain-wide Cas9-mediated therapy for familial Alzheimer’s disease, prime editing for the correction of mutations in adult mice, the restoration of dystrophin expression by suppressing a nonsense mutation via unnatural amino acids, and the reversal of RNA toxicity in myotonic dystrophy via a decoy RNA-binding protein.
本期重點介紹了用于恢復強效腫瘤浸潤淋巴細胞的微流體分選、一天內(nèi)產(chǎn)生強效CAR T細胞、通過腫瘤內(nèi)注射明礬束縛細胞因子的全身抗癌免疫、協(xié)同檢查點阻斷和通過納米級金屬-有機物的放射療法-放射動力學療法框架，通過破壞肝臟中的Cas9活性進行體內(nèi)基因組編輯，全腦Cas9介導的家族性阿爾茨海默氏病治療，用于校正成年小鼠突變的初步編輯，通過抑制無義突變恢復抗肌萎縮蛋白表達非天然氨基酸，以及通過誘餌RNA結合蛋白逆轉(zhuǎn)強直性肌營養(yǎng)不良癥中的RNA毒性。
1.Efficient recovery of potent tumour-infiltrating lymphocytes through quantitative immunomagnetic cell sorting.
通過定量免疫磁細胞分選有效恢復潛在的腫瘤浸潤淋巴細胞。
2.Rapid manufacturing of non-activated potent CAR T cells.
快速生產(chǎn)非激活的有CAR T細胞。
3.Intratumourally injected alum-tethered cytokines elicit potent and safer local and systemic anticancer immunity.
瘤內(nèi)注射明礬束縛細胞因子可引發(fā)有效且更安全的局部和全身抗癌免疫。
4.Synergistic checkpoint-blockade and radiotherapy–radiodynamic therapy via an immunomodulatory nanoscale metal–organic framework.
通過免疫調(diào)節(jié)納米級金屬有機框架協(xié)同檢查點封鎖和放射療法-放射動力學療法。
5.Augmented lipid-nanoparticle-mediated in vivo genome editing in the lungs and spleen by disrupting Cas9 activity in the liver.
通過破壞肝臟中的Cas9活性，增強脂質(zhì)納米顆粒介導的肺和脾臟體內(nèi)基因組編輯。
6.Brain-wide Cas9-mediated cleavage of a gene causing familial Alzheimer’s disease alleviates amyloid-related pathologies in mice.
全腦Cas9介導的導致家族性阿爾茨海默氏病的基因切割減輕了小鼠的淀粉樣蛋白相關病癥。
7.Application of prime editing to the correction of mutations and phenotypes in adult mice with liver and eye diseases.
主要編輯在校正患有肝病和眼病的成年小鼠的突變和表型中的應用。
8.Restoration of dystrophin expression in mice by suppressing a nonsense mutation through the incorporation of unnatural amino acids.
通過摻入非天然氨基酸抑制無義突變，恢復小鼠抗肌萎縮蛋白的表達。
9.Reversal of RNA toxicity in myotonic dystrophy via a decoy RNA-binding protein with high affinity for expanded CUG repeats.
通過對擴增的CUG重復具有高親和力的誘餌RNA結合蛋白逆轉(zhuǎn)強直性營養(yǎng)不良癥中的 RNA毒性。
血清樣本中卵巢癌的光譜指紋圖譜
This issue highlights the performance of paper-based cell-free diagnostics for the detection of the Zika and chikungunya viruses in serum samples, a method for the tracking of thousands of mutations with fewer reads per locus than conventional hybrid-capture duplex sequencing, that ovarian cancer can be predicted via a fingerprint obtained via machine learning from the emissions of carbon-nanotube sensors in serum samples, fast and sensitive electromechanical and biochemical methods for the detection of biomolecules, cell-free diagnostics via multi-arm junction RNAs that execute molecular logic, and the urinary detection of early responses to checkpoint blockade via protease-cleaved antibody-conjugated sensors.
本期重點介紹了基于紙質(zhì)的無細胞診斷檢測血清樣本中寨卡病毒和基孔肯雅病毒的性能，這是一種跟蹤數(shù)千個突變的方法，每個位點的讀取次數(shù)少于傳統(tǒng)的雜交捕獲雙重測序，卵巢癌可以通過機器學習從血清樣本中利用碳納米管傳感器獲得的指紋來預測癌癥，用于檢測生物分子的快速靈敏的機電和生化方法，通過執(zhí)行分子邏輯的多臂連接RNA進行無細胞診斷，以及通過蛋白酶切割的抗體偶聯(lián)傳感器檢測對檢查點封鎖的早期反應的尿液檢測。
1.Field validation of the performance of paper-based tests for the detection of the Zika and chikungunya viruses in serum samples.
在血清樣品中檢測寨卡病毒和基孔肯雅病毒的基于紙質(zhì)測試的性能的現(xiàn)場驗證。
2.Massively parallel enrichment of low-frequency alleles enables duplex sequencing at low depth.
低頻等位基因的大量平行富集可以在低深度下進行雙鏈測序。
3.Detection of ovarian cancer via the spectral fingerprinting of quantum-defect-modified carbon nanotubes in serum by machine learning.
通過機器學習，通過量子缺失改性的碳納米管的光譜指紋識別卵巢癌。
圖1. OOC-DNA納米傳感陣列。a。有機顏色中心-DNA（Organic colour centres，OCC-DNA）納米傳感元素。顯示的是帶有3,4,5-三氟芳基OCC的 ss(GT)15 DNA包裹(6,5)-碳納米管。b。從OCC和ssDNA組件構建OCC-DNA陣列。
4.Rapid and ultrasensitive electromechanical detection of ions, biomolecules and SARS-CoV-2 RNA in unamplified samples.
在未擴增樣品中的離子，生物分子和SARS-COV-2 RNA的快速和超敏機電學檢測。
a．通過MEMS或者NEMS進行生物檢測的原理圖。b. MolEMS及其靜電驅(qū)動的原理圖。單鏈DNA懸臂頂端結合了一個探針，用于特異的生物識別。E代表電場。c. MolEMS g-FET的設備配置。d,e. 設備的照片（d）和光學顯微鏡（e）圖片。f. 固定在MolEMSs上石墨烯的AFM圖片（在1倍的TM緩沖液中）。顏色標尺表明掃描面積的高度。g-i, 使用Cy3-17bp-15T MolEMSs石墨烯的熒光圖片。Vlg是0.9V（g），0V（h）和-1.1V（i）Cy3偶聯(lián)在ssDNA懸臂的頂端作為熒光染料。比例尺，100 nm （f）和 30 μm （e,g–i）.
5.Fast and sensitive detection of SARS-CoV-2 RNA using suboptimal protospacer adjacent motifs for Cas12a.
使用次優(yōu)原型間隔子相鄰基序CAS12a對SARS-COV-2 RNA的快速和敏感檢測。
6.Multi-arm RNA junctions encoding molecular logic unconstrained by input sequence for versatile cell-free diagnostics.
多臂RNA連接編碼分子邏輯的編碼無細胞診斷的輸入序列不受限制的分子邏輯。
7.Urinary detection of early responses to checkpoint blockade and of resistance to it via protease-cleaved antibody-conjugated sensors.
通過蛋白酶切割的抗體偶聯(lián)傳感器對檢查點阻斷的早期反應以及對其抗性的尿液檢測。
