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waters液質(zhì)tof 液相質(zhì)譜重啟流程

2022-11-24 11:42 作者:菜鳥博士_雜貨鋪  | 我要投稿

如何重新啟動(dòng)/開關(guān)電源包含 ACQUITY LC 的 Xevo Tof 系統(tǒng) - WKB24861 目的 重新啟動(dòng)/開關(guān)電源包含 ACQUITY LC 的 Xevo Tof 系統(tǒng)。 環(huán)境 Xevo QTof Xevo G2 Xevo G2-S Xevo G2-XS 所有安裝了 ACQUITY LC 的系統(tǒng) 步驟 將泵手動(dòng)控制開關(guān)(即 Xevo 背面的底部中心位置兩個(gè)切換開關(guān)中的上面一個(gè))翻轉(zhuǎn)到向下位置。您需要將開關(guān)拉出才能對(duì)其進(jìn)行更改。然后,您應(yīng)該能在 Tune 頁(yè)面看到通知,顯示泵手動(dòng)控制處于激活狀態(tài)。 退出 MassLynx 軟件(關(guān)閉 Tune 頁(yè)面、液相方法編輯器、控制臺(tái),然后關(guān)閉 MassLynx)。 運(yùn)行 stopACQUITYProcesses(C:\Program files (x86)\Waters Instruments\Bin\StopACQUITYProcesses.cmd 或 StopProcesses.exe)。 重新啟動(dòng)采集計(jì)算機(jī)。 關(guān)閉 LC 模塊電源。重要說(shuō)明:關(guān)閉 LC 模塊時(shí),請(qǐng)確保連接以太網(wǎng)線纜的模塊(通常為樣品管理器)最后關(guān)閉。 關(guān)閉 LC 模塊大約 30 秒。 重新打開 LC 模塊。重要說(shuō)明:請(qǐng)確保連接以太網(wǎng)線纜的模塊最先打開。 如果是 Xevo G2,請(qǐng)關(guān)閉外部 EPC(所有其它儀器 EPC 將隨電子線路關(guān)閉)。 向下翻轉(zhuǎn)電子線路切換開關(guān)(Xevo 背面兩個(gè)切換開關(guān)中的下面一個(gè))。 等待大約 30 秒。 向上翻轉(zhuǎn) Xevo 背面的電子線路切換開關(guān)。 如果是 Xevo G2,請(qǐng)打開外部 EPC(所有其它儀器 EPC 將隨電子線路打開)。 重要說(shuō)明:等待 5 分鐘,讓 EPC 啟動(dòng)。 打開 MassLynx 軟件。 打開 Tune 頁(yè)面。 打開 View > Vacuum(查看 > 真空),然后打開真空選項(xiàng)卡。確認(rèn) Tof 的真空讀數(shù)為真(非 1e-8)。如果未顯示真實(shí)的 Tof 真空讀數(shù),請(qǐng)?jiān)俅沃匦聠?dòng)。 此外,還需要在 Tune 頁(yè)面中查找錯(cuò)誤信息,顯示泵手動(dòng)控制開關(guān)已處于手動(dòng)控制位置。此錯(cuò)誤信息表明,質(zhì)譜儀和采集計(jì)算機(jī)之間的通訊正確。 顯示良好的真空讀數(shù)后,將泵手動(dòng)控制開關(guān)(上面一個(gè)開關(guān))翻轉(zhuǎn)回自動(dòng)(向上)位置。 確認(rèn)第 17 步中記錄的泵手動(dòng)控制開關(guān)錯(cuò)誤信息已從 Tune 頁(yè)面消失。 將 MS 切換到 Operate(運(yùn)行)模式 How to reboot and power-cycle a Xevo Tof system with ACQUITY LC - WKB24861 Last updatedDec 21, 2021 Save as PDF Share Article number: 24861 OBJECTIVE or GOAL Reboot and power-cycle a Xevo Tof system with ACQUITY LC. ENVIRONMENT Xevo QTof Xevo G2 Xevo G2-S Xevo G2-XS All with ACQUITY LC system attached PROCEDURE Flip the pump override switch (this is the top toggle switch of the two switches in the center at the the bottom on the back of the Xevo) to the down position. You need to pull the switch out to change it. You should then see a notification on the Tune Page that the pump override is active. Exit MassLynx (close the Tune Page, the Inlet Editor, the Console, and then MassLynx). Run stopACQUITYProcesses (C:\Program files (x86)\Waters Instruments\Bin\StopACQUITYProcesses.cmd or StopProcesses.exe). Reboot the acquisition computer. Power-down the LC stack. IMPORTANT: When powering-down the LC stack, ensure that the module with the Ethernet cables attached to it (most commonly the sample manager) is turned off last. Leave the LC stack off for approximately 30 seconds. Turn the LC stack back on. IMPORTANT: Ensure that the module with the Ethernet cables attached to it is turned on first. For a Xevo G2, power-off the external EPC (all other instrument EPCs power-off with the electronics). Flip the electronics toggle switch (the bottom switch of the two toggle switches on the back of the Xevo) down. Wait approximately 30 seconds. Flip the electronics toggle switch on the back of the Xevo up. For a Xevo G2, power-on the external EPC (all other instrument EPCs power-on with the electronics). IMPORTANT: Wait five minutes for the EPC to boot up. Open MassLynx. Open the Tune Page. Go to View > Vacuum, and then the vacuum tab. Verify that the vacuum reading for the Tof is real (not 1e-8). If there is no real Tof vacuum reading, start the reboot again. In the Tune Page, also look for an error message stating that the pump override switch is in the override position. This error message indicates that proper communication exists between the mass spectrometer and the acquisition computer. Once you have a good vacuum reading, flip the pump override switch (the top switch) back to the auto (up) position . Verify that the pump override switch error noted in step 17 is now gone from the Tune Page. Put the MS into Operate mode.

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