SCREEN-WELL自噬文庫：結(jié)構(gòu)和機(jī)械上不同的化合物，用于研究促自噬分子和抗自噬分子在細(xì)胞和體外應(yīng)用中的作用。

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SCREEN-WELL自噬文庫包含94種具有確定的自噬誘導(dǎo)或抑制活性的化合物。將化合物溶解在DMSO或10mM的水中，并以每孔100或500μl的速度等分到深孔板中。包括各種結(jié)構(gòu)和機(jī)械上不同的化合物類別。用于研究細(xì)胞中促自噬和抗自噬分子的作用以及用于體外應(yīng)用的有用工具。

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艾美捷SCREEN-WELL自噬文庫#BML-2837-0100參數(shù)說明：

應(yīng)用：?高溫?超導(dǎo)

內(nèi)容：?94種具有確定的自噬誘導(dǎo)或抑制活性的化合物。包括各種結(jié)構(gòu)和機(jī)械上不同的化合物類別。

數(shù)量：?100?μl/孔或?500?μl/孔

濃度：?DMSO中的?10 mM***

*除非另有說明，否則化合物的濃度主要為?10mM。

**化合物主要以DMSO形式提供。將選定的化合物溶解在無核酸酶的水中。

使用/穩(wěn)定性：?在-80°C下儲(chǔ)存時(shí)，自收到之日起至少可穩(wěn)定一年。

處理：?避免凍融循環(huán)。

航運(yùn)：?干冰

長(zhǎng)期儲(chǔ)存：?-80°C

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SCREEN-WELL自噬文庫部分文獻(xiàn)參考：

1.C9orf72 ALS/FTD dipeptide repeat protein levels are reduced by small molecules that inhibit PKA or enhance protein degradation: N.V. Licata, et al.; EMBO J. 41, e105026 (2022),

2.FUCCI-based live imaging platform reveals cell cycle dynamics and identifies pro-proliferative compounds in human iPSC-derived cardiomyocytes: F. Murganti, et al.; Front. Cardiovasc. Med. 9, 840147 (2022),

3.Rescue of lysosomal function as therapeutic strategy for SPG15 hereditary spastic paraplegia: C. Vantaggiato, et al.; Brain (2022),

4.Autophagy activation, lipotoxicity and lysosomal membrane permeabilization synergize to promote pimozide- and loperamide-induced glioma cell death: N. Meyer, et al.; Autophagy 17, 3424 (2021),

5.Cell death-inducing cytotoxicity in truncated KCNQ4 variants associated with DFNA2 hearing loss: T. Kojima, et al.; Dis. Model. Mech. 14, dmm049015 (2021),

6.Metformin rescues muscle function in BAG3 myofibrillar myopathy models: A.A. Ruparelia, et al.; Autophagy 17, 2494 (2021),

7.Repurposing diphenylbutylpiperidine-class antipsychotic drugs for host-directed therapy of Mycobacterium tuberculosis and Salmonella enterica infections: M.T. Heemskerk, et al.; Sci. Rep. 11, 19634 (2021),

8.Role of ceramide/sphingomyelin (SM) balance regulated through “SM cycle” in cancer: M. Taniguchi, et al.; Cell Signal. 87, 110119 (2021),

9.Phosphorylation of Eukaryotic Initiation factor-2α (eIF2α) in Autophagy: J. Humeau, et al.; Cell Death Dis. 11, 433 (2020),

10.Chemical screening approaches enabling drug discovery of autophagy modulators for biomedical applications in human diseases: P.K. Panda, et al.; Front. Cell. Dev. Biol. 7, 38 (2019),