細(xì)胞培養(yǎng)怎么離得開細(xì)胞因子？ABclonal提供不同種類的科研細(xì)胞因子，涉及生長因子、白介素、腫瘤壞死因子、趨化因子、集落刺激因子、干擾素等種類，可用于刺激細(xì)胞生長和分化、細(xì)胞信號轉(zhuǎn)導(dǎo)以及涉及疾病發(fā)作和進(jìn)展的研究，具有高純度、高活性、低內(nèi)毒素、低批間差的特點，助力廣大科學(xué)研究者的工作！本期精選了一些細(xì)胞因子客戶反饋美圖供大家賞析。

RP01216

1.Active Recombinant Mouse CSF-1/M-CSF Protein

細(xì)胞：小鼠原代骨髓細(xì)胞

描述：Recombinant mouse M-CSF(20 ng/mL) induces mouse primary Bone Marrow cells to differentiate into Macrophages (BMDM). After 7 days, the induction was successful according to cell morphology.

客戶評價：M-CSF可以有效誘導(dǎo)和維持骨髓巨噬細(xì)胞細(xì)胞形態(tài)。

RP01458

2.Active Recombinant Human Mature TGF-beta 1 Protein

細(xì)胞：MC7-F（人乳腺癌細(xì)胞）

圖1

圖2

描述：Active Recombinant Human Mature TGF-beta 1 induces MCF7 cells EMT with a concentration of 12.5 ng/mL. After 10 h, the cell morphology significantly altered, appearing a fusiform shape (figure 1). After 48 h, the change of EMT associated genes are as （figure 2）. Results showed that the induction was successful.

客戶評價：細(xì)胞在誘導(dǎo)后的10H形態(tài)發(fā)生明顯變化，呈現(xiàn)梭狀，形態(tài)偏向于間充質(zhì)細(xì)胞，發(fā)生EMT，但同時也會發(fā)生明顯的細(xì)胞凋亡，可減少TGFB1的濃度，確定合適的誘導(dǎo)時間及誘導(dǎo)濃度。

RP01458

3.Active Recombinant Human Mature TGF-beta 1 Protein

細(xì)胞：HUVEC（人臍靜脈內(nèi)皮細(xì)胞）

描述：Measured by its ability to stimulate smad2/3 autophosphorylation in Human Umbilical Vein Endothelial Cells (HUVEC). 10ng/mL of Recombinant Human TGF-beta1 can effectively enhance smad2/3 autophosphorylation.

客戶評價：刺激成功。

RP01161

4.Active Recombinant Mouse IL-4 Protein

細(xì)胞：C57BL/6J mouse bone marrow cells（C57BL/6J小鼠骨髓細(xì)胞）

描述：Recombinant mouse IL-4(10 ng/mL) and mouse GM-CSF(20 ng/mL) induce C57BL/6J mouse bone marrow cells to differentiate into BMDC. According to the Flowcytometry data, after 6 days, up to 75% of cells in the system expressed DC biomarker CD11C on cell surface, among which more than15% cells had high expression of MHCII. These data shows that the induction was successful.

客戶評價：誘導(dǎo)成功。

RP01206 / RP01161

5.Active Recombinant Mouse CSF-2/GM-CSF Protein

Active Recombinant Mouse IL-4 Protein

細(xì)胞：C57BL/6小鼠骨髓細(xì)胞

描述：Recombinant Mouse IL-4 protein (10 ng/mL) and Mouse GM-CSF protein (20 ng/mL)induce C57BL/6 Mouse bone marrow cells to differentiate into BMDC. After 7 days, the BMDC biomarkers, CD11c, CD80 and CD86 on cell surface are 89.4%, 80.7% and 69.7% respectively.

客戶評價：效果很好。

RP01070

6.Active Recombinant Mouse IFN-gamma Protein

細(xì)胞：BMDM（小鼠骨髓巨噬細(xì)胞）和PeritonealMacrophages（小鼠腹腔巨噬細(xì)胞）

描述：Recombinant mouse IFN-gamma stimulates mouse bone marrow-derived macrophages(Figure 1) and peritoneal macrophages (Figure 2) to differentiation into M1 Macrophages. The expression of related biomarkers TNF-α, Nos2, (IL-12), IL-1β, and IL-6 was detected to be significantly upregulated.

客戶評價：相關(guān)標(biāo)記物上調(diào)十分顯著。

RP01345

7.Active Recombinant Mouse FGF-2/bFGF Protein

細(xì)胞：DPcell（真皮乳頭細(xì)胞）

描述：Recombinant mouse FGF2 protein was added during DP cell culture. The immunofluorescence identification showed that 1 ng/mL FGF2 could maintain the cellular characteristics of DP cells. The red part is the fluorescently labeled PDFGRA antibody, and the blue-cyan part is the DAPI-stained cells.

客戶評價：FGF2細(xì)胞因子可以使其維持真皮乳頭的細(xì)胞特征。

END

ABclonal

公司簡介

愛博泰克生物科技有限公司（ABclonal Technology Co.,Ltd.）成立于2011年，作為生命科學(xué)解決方案的提供商，旨在為更多的科研工作者、IVD企業(yè)、新技術(shù)開發(fā)企業(yè)提供抗體、酶、NGS產(chǎn)品和蛋白制品等全套解決方案。