3D?Fect? 適用于各種支架和細(xì)胞。這種3D轉(zhuǎn)染試劑是專門開發(fā)的，用于直接轉(zhuǎn)染在3D支架中培養(yǎng)的細(xì)胞。該試劑基于一種新技術(shù)，為細(xì)胞培養(yǎng)和轉(zhuǎn)染增加了第三維度。3D矩陣不僅為細(xì)胞的環(huán)境增加了第三個(gè)維度，還允許在細(xì)胞特征和行為方面產(chǎn)生顯著差異。

?

由于3D基質(zhì)通常用于基礎(chǔ)研究和治療應(yīng)用，OZ Biosciences開發(fā)了3D Fect? 3D支架專用的轉(zhuǎn)染試劑。通過這種方式，使用3D Fect激活基于支架的3D矩陣?/DNA復(fù)合物被細(xì)胞定植，隨后在更自然的環(huán)境中原位轉(zhuǎn)染。這種方法可以研究組織工程和再生、腫瘤侵襲、神經(jīng)分化、細(xì)胞極化、組織形成、定植、軸突生長(zhǎng)...

?

艾美捷OZ Biosciences?3D Fect轉(zhuǎn)染試劑特色：

高效：細(xì)胞系和原代細(xì)胞

3D腳手架專用

與所有類型的核酸兼容

完全可生物降解

長(zhǎng)期蛋白質(zhì)表達(dá)

血清相容性

?

OZ Biosciences?3D Fect轉(zhuǎn)染試劑?#OZB-TF21000規(guī)格：

250μL：適用于65至125個(gè)含有1μg DNA的轉(zhuǎn)染

500μL：適用于125至250個(gè)含有1μg DNA的轉(zhuǎn)染

1000μL：適用于250至500個(gè)含有1μg DNA的轉(zhuǎn)染

?

OZ Biosciences?3D Fect轉(zhuǎn)染試劑發(fā)表文章：

細(xì)胞名稱?核酸類型?產(chǎn)品?參考文章

CLU-301?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HEK-293?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MDCK?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

MEF?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells?3D Transfection DNA?3D-Fect?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1?siRNA?3D-Fect?Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

3T3?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

AdSC?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

BMSC?3D Transfection DNA?3D-FectIN?Sukarto A., Eur Cells and Mat. 2011; 22(4):28.

Chondrocytes?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-1?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

COS-7?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HeLa?3D Transfection siRNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

HMEC-1?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Mesenchymal Stem Cells?mRNA?3D-FectIN?Ledo AM. Acta Biomater . 2020 May 15;S1742-7061(20)30224-5.

Mesenchymal Stem Cells?mRNA?3D-FectIN?Ledo AM., Biomaterials. 2020 Jul;247:120016.

Mesenchymal Stem Cells?3D Transfection DNA?3D-FectIN?Plank C., Eur. J. Nanomed. 2012;4(1):17–32

Mesenchymal Stem Cells?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

Neural Stem Cells?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

NIH-3T3?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RAW 264.7?3D Transfection DNA?3D-FectIN?Sapet C., Ther Deliv. 2013 Jun;4(6):673-85.

RWPE1?siRNA?3D-FectIN?Laperrousaz B., Nucleic Acids Res. 2018 Jan 31. doi: 10.1093/nar/gky030.

ATDC5?siRNA?si3D-FectIN?Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.

Chondrocytes?siRNA?si3D-FectIN?Mori H., Am J Pathol. 2015 Aug 10. pii: S0002-9440(15)00385-5.