ADI-900-204血管緊張素ⅡELISA試劑盒：

Sensitivity:?4.6 pg/ml (range 3.9-10,000 pg/ml)

Assay Time:?3.5 hours

Applications:?ELISA, Colorimetric detection

Application Notes:?For the quantitative determination of species independent Angiotensin II in plasma and serum samples.

Wavelength:?450 nm

Species reactivity:?Species independent

Crossreactivity:?Angiotensin II (100%), Angiotensin A (100%), Angiotensin III (100%), Angiotensin IV (100%), Angiotensin I (0.319%), Angiotensin (1-9) (0.103%), LVV-hemorphin 7 (0.067%), Angiotensin (1-7) (0.053%), Bradykinin (0.022%)

Quantity:?1 x 96-well plate

Use/Stability:?Store all components at +4oC, except Standard at -20oC.

Shipping:?Shipped on Blue Ice

Contents:?Microtiter Plate, Conjugate, Antibody, Assay Buffer 16, Wash Buffer Concentrate, Standard, TMB Substrate, Stop Solution 2, Streptavidin-HRP

Scientific Background:?Angiotensins are small peptides derived from angiotensinogen. Several of the known angiotensins are established endocrine effectors in the regulation of blood pressure, but they are also known to have other functions locally, in several organs and tissues (paracrine) and at the cellular level (autocrine / intracrine). Angiotensin I (DRVYIHPFHL) has no known effector function but it is an immediate precursor of Angiotensin II (DRVYIHPF). Angiotensin II binds AT1 receptors, which promote vasoconstriction, sodium retention, release of aldosterone, release of Arg-vasopressin, cell proliferation, inflammation, fibrosis, anxiety, and cardiac hypertrophy. Angiotensin A (ARVYIHPF), and Angiotensin III (RVYIHPF), also bind AT1 receptors. Angiotensin (1-7) (DRVYIHP) binds a different receptor called MAS-1 which has opposite effects (vasodilation, natriuresis, antiproliferation, NO release, PGE release, and apoptosis). Angiotensin IV (VYIHPF) binds yet another receptor called AT4 (IRAP), which promotes increase of blood flow, angiogenesis, and natriuresis, and which has also been implicated in memory formation and in the pathogenesis of Alzheimer’s disease. The peptide LVV-hemorphin-7 (LVVYPWTQRF), which is not an angiotensin, also binds the AT4 receptor. There are other angiotensin peptides that have been identified, including Ang (1-9) (DRVYIHPFH), Ang (1-12) (DRVYIHPFHLVI), Ang V(3-7) (VYIHP), as well as several other shorter peptides that have undetermined functions.

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艾美捷ADI-900-204?血管緊張素ⅡELISA試劑盒，高靈敏度ELISA，檢測量低至4.6pg/ml，該試劑盒是一種比色、競爭性免疫測定試劑盒，3.5小時內(nèi)即可獲得結(jié)果。

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ADI-900-204血管緊張素ⅡELISA試劑盒化學(xué)性質(zhì)：

靈敏度：4.6 pg/ml（范圍3.9-10000 pg/ml）

化驗時間：3.5小時

應(yīng)用：ELISA，比色檢測

應(yīng)用說明：用于定量測定血漿和血清樣品中的種類無關(guān)的血管緊張素II。

波長：450 nm

物種反應(yīng)性：物種無關(guān)

交叉反應(yīng)性：血管緊張素II（100%），血管緊張素A（100%）、血管緊張素III（100%）和血管緊張素IV（100%）

數(shù)量：1 x 96孔板

使用/穩(wěn)定性：將所有組件儲存在+4oC，標(biāo)準(zhǔn)組件除外，儲存在-20oC。

運(yùn)輸：藍(lán)冰運(yùn)輸

內(nèi)容：微量滴定板、綴合物、抗體、測定緩沖液16、洗滌緩沖液濃縮液、標(biāo)準(zhǔn)品、TMB底物、停止溶液2、鏈霉親和素HRP

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血管緊張素ⅡELISA試劑盒文獻(xiàn)參考：

氯沙坦和維生素D抑制散發(fā)性癌癥條件Apc缺失小鼠模型中結(jié)腸癌的發(fā)展：U.Dougherty等人。；癌癥預(yù)防。Res.（Phila.）12433（2019）；

microRNA-25表達(dá)的改變通過腎素分泌調(diào)節(jié)心臟功能：H.Li等人。；實驗細(xì)胞研究395119（2018）；

與葡萄糖一起攝入全蛋或蛋清可防止餐后高血糖引起的氧化應(yīng)激和精氨酸代謝失調(diào)，并改善糖尿病前期男性的血管內(nèi)皮功能：J.D.McDonald等人。；《營養(yǎng)學(xué)雜志》。120901（2018），應(yīng)用：人血漿；

硫化氫減弱腎素-血管緊張素和醛固酮病理信號，以保護(hù)腎功能并改善心臟的運(yùn)動耐受性：Z.Li，et al。；JACC基礎(chǔ)翻譯。科學(xué)。3，796（2018），應(yīng)用：使用小鼠腎臟的ELISA