【標題速讀】【Nprot】【2022年】【2-6月】
聲明：本專欄主要對生命科學(xué)領(lǐng)域的一些期刊文章標題進行翻譯，所有內(nèi)容均由本人手工整理翻譯。由于本人專業(yè)為生物分析相關(guān)，其他領(lǐng)域如果出現(xiàn)翻譯錯誤請諒解。
多種人體組織的多重成像
Rainbow collage of IBEX images from nine different human tissues, including the lymph node, thymus, spleen, jejunum, kidney, liver, skin and heart. Individual images display unique cell types and anatomical structures defined by a single protein biomarker. All protein biomarkers are targeted by commercially available antibodies and obtained by IBEX, an open-source multiplexed imaging method.
來自九種不同人體組織的IBEX圖像的彩虹拼貼畫，包括淋巴結(jié)、胸腺、脾臟、空腸、腎臟、肝臟、皮膚和心臟。 單個圖像顯示由單個蛋白質(zhì)生物標志物定義的獨特細胞類型和解剖結(jié)構(gòu)。所有蛋白質(zhì)生物標志物均以市售抗體為目標，并通過開源多重成像方法IBEX獲得。
1.Routine culture and study of adult human brain cells from neurosurgical specimens.
對來自神經(jīng)外科標本的成年人類腦細胞進行常規(guī)培養(yǎng)和研究。
2.Neuronal subtype-specific growth cone and soma purification from mammalian CNS via fractionation and fluorescent sorting for subcellular analyses and spatial mapping of local transcriptomes and proteomes.
通過分餾和熒光分選從哺乳動物中樞神經(jīng)系統(tǒng)純化神經(jīng)元亞型特異性生長錐和體細胞，用于亞細胞分析和局部轉(zhuǎn)錄組和蛋白質(zhì)組的空間映射。
3.CRISPRi-seq for genome-wide fitness quantification in bacteria.
CRISPRi-seq用于細菌的全基因組適應(yīng)性量化。
4.Designer DNA nanostructures for viral inhibition.
用于抑制病毒的DNA納米結(jié)構(gòu)的設(shè)計。
5.Protein transfection via spherical nucleic acids.
通過球形核酸的蛋白質(zhì)轉(zhuǎn)染。
6.High-yield production of mono- or few-layer transition metal dichalcogenide nanosheets by an electrochemical lithium ion intercalation-based exfoliation method.
基于電化學(xué)的鋰離子插層剝離法高產(chǎn)生產(chǎn)單層或少層過渡金屬二氯化物納米片。
7.IBEX: an iterative immunolabeling and chemical bleaching method for high-content imaging of diverse tissues.
IBEX：一種迭代的免疫標記和化學(xué)漂白方法，用于不同組織的高含量成像。
8.KAS-seq: genome-wide sequencing of single-stranded DNA by N3-kethoxal–assisted labeling.
KAS-seq：通過N3-酮醇輔助標記對單鏈DNA進行全基因組測序。
9.High-resolution in situ structure determination by cryo-electron tomography and subtomogram averaging using emClarity.
使用emClarity通過低溫電子斷層掃描和子圖平均化進行高分辨率原位結(jié)構(gòu)測定。
10.Capture-C: a modular and flexible approach for high-resolution chromosome conformation capture.
Capture-C：一種用于高分辨率染色體構(gòu)象捕獲的模塊化和靈活方法。
11.Pooled genetic perturbation screens with image-based phenotypes.
基于圖像的表型的集合遺傳擾動篩選。
12.Continuous human iPSC-macrophage mass production by suspension culture in stirred tank bioreactors.
在攪拌罐生物反應(yīng)器中通過懸浮培養(yǎng)連續(xù)生產(chǎn)人類iPSC-巨噬細胞。
13.Stabilization and structure determination of integral membrane proteins by termini restraining.
通過端部抑制來穩(wěn)定和確定整體膜蛋白的結(jié)構(gòu)。
用FLARE染色的人乳腺組織
Formalin-fixed paraffin-embedded human breast tissue that was rapidly stained by FLARE (fluorescent labeling of abundant reactive entities), enabling informative visualization analogous to classic histology stains and holding the potential to revolutionize the clinical practice of examining tissue samples.
福爾馬林固定石蠟包埋的人乳腺組織被FLARE（大量反應(yīng)實體的熒光標記）快速染色，實現(xiàn)類似于經(jīng)典組織學(xué)染色的信息可視化，并有可能徹底改變檢查組織樣本的臨床實踐。
1.Metabolic labeling of secreted matrix to investigate cell–material interactions in tissue engineering and mechanobiology.
分泌基質(zhì)的代謝標記，研究組織工程和機械生物學(xué)中的細胞-材料相互作用。
2.Transplantation of intestinal organoids into a mouse model of colitis.
將腸道器官移植到小鼠結(jié)腸炎模型中。
3.Artificial intelligence–enabled virtual screening of ultra-large chemical libraries with deep docking.
人工智能驅(qū)動的超大型化學(xué)庫的虛擬篩選與深度對接。
4.Universal platform for the generation of thermostabilized GPCRs that crystallize in LCP.
用于生成在LCP中結(jié)晶的熱穩(wěn)定GPCR的通用平臺。
5.Quantum defects as versatile anchors for carbon nanotube functionalization.
量子缺陷作為碳納米管功能化的多功能錨。
6.Synthesis of siRNA nanoparticles to silence plaque-destabilizing gene in atherosclerotic lesional macrophages.
合成siRNA納米顆粒以沉默動脈粥樣硬化病變巨噬細胞中的斑塊穩(wěn)定基因。
7.dCas9-VPR-mediated transcriptional activation of functionally equivalent genes for gene therapy.
dCas9-VPR介導(dǎo)的功能等同基因的轉(zhuǎn)錄激活用于基因治療。
8.Fluorescent labeling of abundant reactive entities (FLARE) for cleared-tissue and super-resolution microscopy.
豐富的反應(yīng)性實體的熒光標記（FLARE）用于干凈的組織和超分辨率顯微鏡。
9.High-resolution imaging of bacterial spatial organization with vertical cell imaging by nanostructured immobilization (VerCINI).
通過納米結(jié)構(gòu)固定化的垂直細胞成像（VerCINI）對細菌空間組織進行高分辨成像。
10.Global mapping of RNA G-quadruplexes (G4-RNAs) using G4RP-seq.
使用G4RP-seq對RNA G-四重體（G4-RNAs）進行全球測繪。
11.Spotting-based differentiation of functional dopaminergic progenitors from human pluripotent stem cells.
基于斑點的人類多能干細胞功能多巴胺能祖細胞的分化。
12.3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert.
人類腸道上皮細胞在腸道芯片或帶有細胞培養(yǎng)插件的混合芯片中的三維體外形態(tài)發(fā)生。
具有實時刺激控制的無線有源光遺傳學(xué)裝置
The layered schematics of a wireless optogenetic device capable of full subdermal implantation over the skull with real-time control over optical modulation on neural activities. Application of such devices in live rodent models enables profound behavior studies that explore the underlying neural principles behind outcome behaviors.
無線光遺傳學(xué)設(shè)備的分層示意圖，能夠在頭骨上完全皮下植入，實時控制神經(jīng)活動的光學(xué)調(diào)制。在活的嚙齒動物模型中應(yīng)用此類設(shè)備可以進行深入的行為研究，探索結(jié)果行為背后的潛在神經(jīng)原理。
1.Preparing ductal epithelial organoids for high-spatial-resolution molecular profiling using mass spectrometry imaging.
利用質(zhì)譜成像技術(shù)為高空間分辨率的分子分析準備導(dǎo)管上皮細胞器官。
2.Synthesis of 68Ga-radiopharmaceuticals using both generator-derived and cyclotron-produced 68Ga as exemplified by [68Ga]Ga-PSMA-11 for prostate cancer PET imaging.
以用于前列腺癌PET成像的[68Ga]Ga-PSMA-11為例，利用發(fā)生器產(chǎn)生的和回旋加速器產(chǎn)生的68Ga合成68Ga放射性藥物。
3.Generation of CRISPR–Cas9-mediated genetic knockout human intestinal tissue–derived enteroid lines by lentivirus transduction and single-cell cloning.
通過慢病毒轉(zhuǎn)導(dǎo)和單細胞克隆產(chǎn)生CRISPR-Cas9介導(dǎo)的基因敲除人類腸道組織衍生的腸系。
4.Bioconjugates of photon-upconversion nanoparticles for cancer biomarker detection and imaging.
用于癌癥生物標志物檢測和成像的光子上轉(zhuǎn)換納米粒子的生物結(jié)合物。
5.Preparation and use of wireless reprogrammable multilateral optogenetic devices for behavioral neuroscience.
用于行為神經(jīng)科學(xué)的無線可重復(fù)編程多邊光遺傳裝置的制備和使用。
6.Genetic circuit design automation with Cello 2.0.
用Cello 2.0實現(xiàn)遺傳電路設(shè)計自動化。
7.Accurate determination of protein:ligand standard binding free energies from molecular dynamics simulations.
從分子動力學(xué)模擬中精確確定蛋白質(zhì)：配體標準結(jié)合自由能。
8.CRISPR somatic genome engineering and cancer modeling in the mouse pancreas and liver.
小鼠胰腺和肝臟的CRISPR體細胞基因組工程和癌癥模型的建立。
有毛發(fā)的皮膚類器官
The image displays a human-stem-cell-derived skin organoid with radially growing hair follicles (green) and nerves (red/yellow). This particular organoid was grown from a genetically modified stem cell line where the Desmoplakin gene/protein is tagged with a GFP. As a consequence, the numerous desmosomes (cell–cell junctions) in the epidermis of the organoid glow green. Neurons are labeled with an antibody for beta-III tubulin.
該圖像顯示了人類干細胞衍生的皮膚類器官，具有徑向生長的毛囊（綠色）和神經(jīng)（紅色/黃色）。這種特殊的類器官是從轉(zhuǎn)基因干細胞系中生長出來的，其中Desmoplakin基因/蛋白被GFP標記。因此，類器官表皮中的大量橋粒（細胞-細胞連接）發(fā)出綠色光。神經(jīng)元用β-III微管蛋白抗體標記。
1.Transcriptome-wide identification of RNA-binding protein binding sites using seCLIP-seq.
利用seCLIP-seq在整個轉(zhuǎn)錄組中鑒定RNA結(jié)合蛋白的結(jié)合點。
2.Generation and characterization of hair-bearing skin organoids from human pluripotent stem cells.
來自人類多能干細胞的毛發(fā)皮膚器官的生成和特征。
3.RASER-FISH: non-denaturing fluorescence in situ hybridization for preservation of three-dimensional interphase chromatin structure.
RASER-FISH：非變性熒光原位雜交技術(shù)保存三維間期染色質(zhì)結(jié)構(gòu)。
4.CRISPR–Cas9-mediated chromosome engineering in Arabidopsis thaliana.
CRISPR-Cas9介導(dǎo)的擬南芥染色體工程。
5.Isolation of mouse pancreatic islet Procr+ progenitors and long-term expansion of islet organoids in vitro.
小鼠胰島Procr+祖細胞的分離和體外胰島器官的長期擴增。
表征細胞類型特異性染色質(zhì)景觀
ATAC-seq profiles the gene regulatory ‘landscape’ of a cell. Promoters, enhancers and other putative gene regulatory elements are identified as peaks in the data and these signals are highly cell-type-specific. Here, the skylines of Paris, San Francisco, New York City and Shanghai are depicted in the style of cell-type-specific ATAC-seq peaks.
ATAC-seq描述了細胞的基因調(diào)控“景觀”。啟動子、增強子和其他假定的基因調(diào)控元件被識別為數(shù)據(jù)中的峰值，這些信號具有高度的細胞類型特異性。在這里，巴黎、舊金山、紐約市和上海的天際線以細胞類型特定的ATAC-seq峰的風(fēng)格描繪。
1.Rapid in situ identification of biological specimens via DNA amplicon sequencing using miniaturized laboratory equipment.
利用小型化實驗室設(shè)備通過DNA擴增子測序?qū)ι飿吮具M行快速原位鑒定。
2.Purification of mammalian telomeric DNA for single-molecule analysis.
純化哺乳動物端粒DNA進行單分子分析。
3.Subretinal injection in mice to study retinal physiology and disease.
小鼠視網(wǎng)膜下注射，研究視網(wǎng)膜的生理和疾病。
4.Sister chromatid–sensitive Hi-C to map the conformation of replicated genomes.
姐妹染色體敏感的Hi-C來繪制復(fù)制基因組的構(gòu)象。
5.Chromatin accessibility profiling by ATAC-seq.
通過ATAC-seq進行染色質(zhì)可及性分析。
